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The map label for this gene is ypfH [H]

Identifier: 238895933

GI number: 238895933

Start: 3881807

End: 3882556

Strand: Reverse

Name: ypfH [H]

Synonym: KP1_4058

Alternate gene names: 238895933

Gene position: 3882556-3881807 (Counterclockwise)

Preceding gene: 238895934

Following gene: 238895928

Centisome position: 73.97

GC content: 57.6

Gene sequence:

>750_bases
ATGGTCATATCTGGCGAGCGGCGTACACTGCCCGCAAACACTAAGGAGACAGCCATGAAACACGATCATTTTATTGTTCA
AAGTCCTGCGACGCCTGCGCAACAGCTGTTGCTGCTGTTTCATGGCGTAGGGGATAACCCGGTCTCCATGGGGCAAATCG
GCAGCTGGTTCGCGCCGCAGTTTCCTGATGCGCTGATCGTCAGCATTGGCGGGGTGGAACCTTGCGGCCCCAACGGTCGG
CAGTGGTTTTCACTGCAGGGGGTGACGGAAGAGAATCGTCAGCAGCGCATCGACGCCATTATGCCGACCTTTATTGAAAT
TGTACGCTACTGGCAGGAAAAGAGCGGCGTCAGTCCACTGGCCACCGCGCTGATCGGTTTCTCTCAGGGGGCGATTATGT
CGCTGGAGAGTATCAAAGCGGCGCCGGATCTGGCTTCGCGGGTCATCGCCTTTAATGGCCGCTATGCGACGCTGCCGGAG
ACGGCAACGACGGCCACCACCATTCACCTGATCCACGGTGGTGAAGATCGGGTTATCGACCTGGCGTGGGCGGTGTCGGC
CGAGGAGGCGTTACAGCAGGCGGGCGGCGACGTGACCTTAGATATTGTCGACGATCTCGGGCATGCCATTGACGATCGCA
GTATGCAGTTGGCGATCGAGCGTCTGCGTTATACCGTGCCGAAGCACTATTTTGATGAGGCGCTGAGCGGCAGTACGCCA
AAAGGGGATGATATCATCGAAATGCTGTAA

Upstream 100 bases:

>100_bases
GCTGACGCCTTGCGCTCCCTTGATGCAGCGCGCGCTGATGCGCTGCGTCAGCAGGTGGCGCATCTGCAATTTTTTTAACT
AACTCATTCAGTTAAATGGC

Downstream 100 bases:

>100_bases
CCCCGGAGAGCGGGGTTACAGGCGGGCGGTACCGCGATAAAGGCAAATTTACGGTTTCTTCGACTGGTCGTGCTTCGGCC
AGTCGTCGTCATCGTCCCAT

Product: esterase YpfH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MVISGERRTLPANTKETAMKHDHFIVQSPATPAQQLLLLFHGVGDNPVSMGQIGSWFAPQFPDALIVSIGGVEPCGPNGR
QWFSLQGVTEENRQQRIDAIMPTFIEIVRYWQEKSGVSPLATALIGFSQGAIMSLESIKAAPDLASRVIAFNGRYATLPE
TATTATTIHLIHGGEDRVIDLAWAVSAEEALQQAGGDVTLDIVDDLGHAIDDRSMQLAIERLRYTVPKHYFDEALSGSTP
KGDDIIEML

Sequences:

>Translated_249_residues
MVISGERRTLPANTKETAMKHDHFIVQSPATPAQQLLLLFHGVGDNPVSMGQIGSWFAPQFPDALIVSIGGVEPCGPNGR
QWFSLQGVTEENRQQRIDAIMPTFIEIVRYWQEKSGVSPLATALIGFSQGAIMSLESIKAAPDLASRVIAFNGRYATLPE
TATTATTIHLIHGGEDRVIDLAWAVSAEEALQQAGGDVTLDIVDDLGHAIDDRSMQLAIERLRYTVPKHYFDEALSGSTP
KGDDIIEML
>Mature_249_residues
MVISGERRTLPANTKETAMKHDHFIVQSPATPAQQLLLLFHGVGDNPVSMGQIGSWFAPQFPDALIVSIGGVEPCGPNGR
QWFSLQGVTEENRQQRIDAIMPTFIEIVRYWQEKSGVSPLATALIGFSQGAIMSLESIKAAPDLASRVIAFNGRYATLPE
TATTATTIHLIHGGEDRVIDLAWAVSAEEALQQAGGDVTLDIVDDLGHAIDDRSMQLAIERLRYTVPKHYFDEALSGSTP
KGDDIIEML

Specific function: Displays esterase activity toward palmitoyl-CoA and pNP- butyrate [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI87082111, Length=232, Percent_Identity=79.3103448275862, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 27093; Mature: 27093

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISGERRTLPANTKETAMKHDHFIVQSPATPAQQLLLLFHGVGDNPVSMGQIGSWFAPQ
CEECCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
FPDALIVSIGGVEPCGPNGRQWFSLQGVTEENRQQRIDAIMPTFIEIVRYWQEKSGVSPL
CCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ATALIGFSQGAIMSLESIKAAPDLASRVIAFNGRYATLPETATTATTIHLIHGGEDRVID
HHHHHHCCCCHHHHHHHHHCCHHHHHHHEEECCCEEECCCCCCCEEEEEEEECCCCCEEE
LAWAVSAEEALQQAGGDVTLDIVDDLGHAIDDRSMQLAIERLRYTVPKHYFDEALSGSTP
EEECCCHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
KGDDIIEML
CCCHHHHCC
>Mature Secondary Structure
MVISGERRTLPANTKETAMKHDHFIVQSPATPAQQLLLLFHGVGDNPVSMGQIGSWFAPQ
CEECCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
FPDALIVSIGGVEPCGPNGRQWFSLQGVTEENRQQRIDAIMPTFIEIVRYWQEKSGVSPL
CCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ATALIGFSQGAIMSLESIKAAPDLASRVIAFNGRYATLPETATTATTIHLIHGGEDRVID
HHHHHHCCCCHHHHHHHHHCCHHHHHHHEEECCCEEECCCCCCCEEEEEEEECCCCCEEE
LAWAVSAEEALQQAGGDVTLDIVDDLGHAIDDRSMQLAIERLRYTVPKHYFDEALSGSTP
EEECCCHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
KGDDIIEML
CCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]