Click here to switch to the map view.

The map label for this gene is prfA [H]

Identifier: 238895295

GI number: 238895295

Start: 3178239

End: 3179321

Strand: Reverse

Name: prfA [H]

Synonym: KP1_3349

Alternate gene names: 238895295

Gene position: 3179321-3178239 (Counterclockwise)

Preceding gene: 238895296

Following gene: 238895294

Centisome position: 60.58

GC content: 61.68

Gene sequence:

>1083_bases
ATGAAGTCTTCTATTGTTGCCAAACTGGAAGCGTTGTACGAGCGCCATGAAGAAGTGCAGGCGCTGCTCGGCGATGCCGC
GACGATTGCCGATCAGGATAAATTCCGCGCGCTGTCGCGGGAGTATGCTCAACTGAGCGATGTGGCGCGCTGCTATACCG
ACTGGCGTCAGGTGCAGGAGGATATTGAAACCGCGCAGATGATGCTCGACGATCCGGAAATGCGCGAAATGGCGCAGGAA
GAGCTGCGTGACGCCAAAGAAAAAGGCGACCAGCTGGAGCAACAACTGCAGGTTCTCCTCCTGCCGAAAGATCCTGATGA
CGAGCGTAACGCCTTCGTGGAAGTGCGCGCCGGGACCGGCGGCGACGAAGCAGCGCTGTTTGCCGGCGATCTATTCCGCA
TGTACACCCGTTACGCAGAATCGCGCCGCTGGCAGGTAGAGATCCTCAGCGCCAACGAAGGTGAGCACGGCGGCTTTAAA
GAGGTGATCGCCAAGATCAGCGGTGACGGCGTCTATGGTCGGCTCAAATTTGAATCCGGCGGTCATCGCGTGCAGCGCGT
GCCGGCCACCGAGTCGCAGGGGCGGATCCACACCTCCGCCTGTACGGTGGCGGTGATGCCGGAGCTGCCGGAAGCCGAAA
TGCCGGACATTAACCCGGCGGATCTGCGCATCGATACCTTCCGTTCCTCCGGGGCAGGCGGTCAGCACGTTAACACCACC
GACTCGGCTATCCGCATTACCCACCTGCCCACCGGCATCGTGGTGGAGTGCCAGGACGAGCGTTCTCAGCATAAAAACAA
AGCCAAGGCGCTGTCGGTGCTGGGCGCGCGCATCCGCGCCGCCGAAGTGGCCAAACGTCAGCAGGCGGAAGCCTCCACGC
GCCGCAACCTGCTGGGCAGCGGCGACCGCAGCGACCGCAATCGCACCTATAACTTCCCGCAGGGCCGCGTGACCGACCAC
CGCATCAACCTGACGCTCTACCGTCTGGATGAGGCGATGGAGGGCAAACTCGACATGCTGATTGAGCCGATTGTCCAGGA
ACATCAGGCCGATCAGCTGGCGGCGCTGTCCGAGCAGGAATGA

Upstream 100 bases:

>100_bases
CCGTGACGGGGACGACGAACGTCTGCATATTCTGCGCAACAGCCTCGGGCTGGAATAGCGCACCTTTCTCTTATTTTCAG
GACAGGGTGAATTCACGCCT

Downstream 100 bases:

>100_bases
TGACCTTTCAGGCCTGGCTGCAGCAGGCGATCGCCCGCCTGGCGGAGAGCGACAGCCCGCGACGCGACGCCGAGATCCTG
CTGGGACACGTTACCGGCCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKSSIVAKLEALYERHEEVQALLGDAATIADQDKFRALSREYAQLSDVARCYTDWRQVQEDIETAQMMLDDPEMREMAQE
ELRDAKEKGDQLEQQLQVLLLPKDPDDERNAFVEVRAGTGGDEAALFAGDLFRMYTRYAESRRWQVEILSANEGEHGGFK
EVIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPEAEMPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIRAAEVAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEAMEGKLDMLIEPIVQEHQADQLAALSEQE

Sequences:

>Translated_360_residues
MKSSIVAKLEALYERHEEVQALLGDAATIADQDKFRALSREYAQLSDVARCYTDWRQVQEDIETAQMMLDDPEMREMAQE
ELRDAKEKGDQLEQQLQVLLLPKDPDDERNAFVEVRAGTGGDEAALFAGDLFRMYTRYAESRRWQVEILSANEGEHGGFK
EVIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPEAEMPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIRAAEVAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEAMEGKLDMLIEPIVQEHQADQLAALSEQE
>Mature_360_residues
MKSSIVAKLEALYERHEEVQALLGDAATIADQDKFRALSREYAQLSDVARCYTDWRQVQEDIETAQMMLDDPEMREMAQE
ELRDAKEKGDQLEQQLQVLLLPKDPDDERNAFVEVRAGTGGDEAALFAGDLFRMYTRYAESRRWQVEILSANEGEHGGFK
EVIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPEAEMPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIRAAEVAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEAMEGKLDMLIEPIVQEHQADQLAALSEQE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=49.8269896193772, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI34577120, Length=340, Percent_Identity=40.2941176470588, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=50, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=92.7777777777778, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=38.7573964497041, Blast_Score=204, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=353, Percent_Identity=32.5779036827195, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=42.1725239616613, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI19921226, Length=322, Percent_Identity=40.9937888198758, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40363; Mature: 40363

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSIVAKLEALYERHEEVQALLGDAATIADQDKFRALSREYAQLSDVARCYTDWRQVQE
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAQMMLDDPEMREMAQEELRDAKEKGDQLEQQLQVLLLPKDPDDERNAFVEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYTRYAESRRWQVEILSANEGEHGGFKEVIAKISGDGVYGRLKFESG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPELPEAEMPDINPADLRIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIRAAEVAKRQQAEASTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEAMEGKLDMLIEPIVQEHQADQLAALSEQE
CCCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHCCHHHHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure
MKSSIVAKLEALYERHEEVQALLGDAATIADQDKFRALSREYAQLSDVARCYTDWRQVQE
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAQMMLDDPEMREMAQEELRDAKEKGDQLEQQLQVLLLPKDPDDERNAFVEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYTRYAESRRWQVEILSANEGEHGGFKEVIAKISGDGVYGRLKFESG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPELPEAEMPDINPADLRIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIRAAEVAKRQQAEASTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEAMEGKLDMLIEPIVQEHQADQLAALSEQE
CCCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHCCHHHHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA