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The map label for this gene is hemK [H]

Identifier: 238895294

GI number: 238895294

Start: 3177391

End: 3178239

Strand: Reverse

Name: hemK [H]

Synonym: KP1_3348

Alternate gene names: 238895294

Gene position: 3178239-3177391 (Counterclockwise)

Preceding gene: 238895295

Following gene: 238895293

Centisome position: 60.55

GC content: 66.2

Gene sequence:

>849_bases
ATGACCTTTCAGGCCTGGCTGCAGCAGGCGATCGCCCGCCTGGCGGAGAGCGACAGCCCGCGACGCGACGCCGAGATCCT
GCTGGGACACGTTACCGGCCGGGCGCGAACCTGGATTTTGGCCTTCGGTGAAACGACGCTCTCTGCTGACGAGGCGGCGA
AGCTGGAGGCGCTCCTGGTGCGACGCCAGCGCGGGGAACCTATTGCTCATCTGGTCGGGCAGCGCGAGTTTTGGTCGCTG
CCGCTGTTCGTCTCCCCGGCGACGCTGATCCCCCGCCCGGATACCGAATGTCTGGTAGAGCAGGCGCTGGCGCGTCTGCC
AACCGCGCCGTGCCGTATTCTCGACCTCGGCACCGGGACCGGCGCCATTGCCCTGGCGCTGGCCAGCGAACGTCCGGACT
GTGAGGTCACCGCGGTGGACGTGATGCCGGACGCCGTGGCGCTGGCGTTGCGCAATGCCGAACATCTGAGCATCGCTAAT
GTGACGATAAGTCAAAGCGACTGGTTTAGCGCGCTGGCCGGGCAGCGCTTCGCCACGATCGTCAGCAATCCCCCCTATAT
CGACGCCGCCGACCCGCATCTCGCCGAAGGCGATGTGCGCTTTGAGCCGTTGACGGCGCTGGTCGCAGGCGACCAGGGGC
TGGCCGATCTGGCGCATATCATCCGCGAGGGACGGCAGTATCTGCAGCCCGGCGGTTGGATGCTGCTGGAGCATGGCTGG
ACTCAGGGCGAGGCGGTTCGCGCGCTGTTCCGCGAAGCGGGCTATCTGGACGTCGCGACCTGTCGCGACTATGGCGACAA
TGAGCGTCTGACCCTCGGACGTTTACCCGACATGGAGAATGTTGGCTGA

Upstream 100 bases:

>100_bases
GTCTGGATGAGGCGATGGAGGGCAAACTCGACATGCTGATTGAGCCGATTGTCCAGGAACATCAGGCCGATCAGCTGGCG
GCGCTGTCCGAGCAGGAATG

Downstream 100 bases:

>100_bases
TGAACTTATTCACGGCTGTCCTCTACCTGCACATTGCCACAGTGGCGGTGTCGGTAGGATTGTTCGTTTTACGTTACTGG
TGGATGTATCACCAGAGCCC

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTFQAWLQQAIARLAESDSPRRDAEILLGHVTGRARTWILAFGETTLSADEAAKLEALLVRRQRGEPIAHLVGQREFWSL
PLFVSPATLIPRPDTECLVEQALARLPTAPCRILDLGTGTGAIALALASERPDCEVTAVDVMPDAVALALRNAEHLSIAN
VTISQSDWFSALAGQRFATIVSNPPYIDAADPHLAEGDVRFEPLTALVAGDQGLADLAHIIREGRQYLQPGGWMLLEHGW
TQGEAVRALFREAGYLDVATCRDYGDNERLTLGRLPDMENVG

Sequences:

>Translated_282_residues
MTFQAWLQQAIARLAESDSPRRDAEILLGHVTGRARTWILAFGETTLSADEAAKLEALLVRRQRGEPIAHLVGQREFWSL
PLFVSPATLIPRPDTECLVEQALARLPTAPCRILDLGTGTGAIALALASERPDCEVTAVDVMPDAVALALRNAEHLSIAN
VTISQSDWFSALAGQRFATIVSNPPYIDAADPHLAEGDVRFEPLTALVAGDQGLADLAHIIREGRQYLQPGGWMLLEHGW
TQGEAVRALFREAGYLDVATCRDYGDNERLTLGRLPDMENVG
>Mature_281_residues
TFQAWLQQAIARLAESDSPRRDAEILLGHVTGRARTWILAFGETTLSADEAAKLEALLVRRQRGEPIAHLVGQREFWSLP
LFVSPATLIPRPDTECLVEQALARLPTAPCRILDLGTGTGAIALALASERPDCEVTAVDVMPDAVALALRNAEHLSIANV
TISQSDWFSALAGQRFATIVSNPPYIDAADPHLAEGDVRFEPLTALVAGDQGLADLAHIIREGRQYLQPGGWMLLEHGWT
QGEAVRALFREAGYLDVATCRDYGDNERLTLGRLPDMENVG

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=272, Percent_Identity=30.1470588235294, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1787463, Length=274, Percent_Identity=75.1824817518248, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI87082085, Length=196, Percent_Identity=35.7142857142857, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324266, Length=177, Percent_Identity=29.9435028248588, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582226, Length=240, Percent_Identity=26.25, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30724; Mature: 30593

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQAWLQQAIARLAESDSPRRDAEILLGHVTGRARTWILAFGETTLSADEAAKLEALLV
CCHHHHHHHHHHHHHCCCCCCCCHHEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHH
RRQRGEPIAHLVGQREFWSLPLFVSPATLIPRPDTECLVEQALARLPTAPCRILDLGTGT
HHHCCCHHHHHHCCHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
GAIALALASERPDCEVTAVDVMPDAVALALRNAEHLSIANVTISQSDWFSALAGQRFATI
CEEEEEEECCCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCEEEE
VSNPPYIDAADPHLAEGDVRFEPLTALVAGDQGLADLAHIIREGRQYLQPGGWMLLEHGW
ECCCCCCCCCCCCCCCCCCEECCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
TQGEAVRALFREAGYLDVATCRDYGDNERLTLGRLPDMENVG
CCHHHHHHHHHHCCCEEEEHHCCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TFQAWLQQAIARLAESDSPRRDAEILLGHVTGRARTWILAFGETTLSADEAAKLEALLV
CHHHHHHHHHHHHHCCCCCCCCHHEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHH
RRQRGEPIAHLVGQREFWSLPLFVSPATLIPRPDTECLVEQALARLPTAPCRILDLGTGT
HHHCCCHHHHHHCCHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
GAIALALASERPDCEVTAVDVMPDAVALALRNAEHLSIANVTISQSDWFSALAGQRFATI
CEEEEEEECCCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCEEEE
VSNPPYIDAADPHLAEGDVRFEPLTALVAGDQGLADLAHIIREGRQYLQPGGWMLLEHGW
ECCCCCCCCCCCCCCCCCCEECCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
TQGEAVRALFREAGYLDVATCRDYGDNERLTLGRLPDMENVG
CCHHHHHHHHHHCCCEEEEHHCCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]