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The map label for this gene is mhpB [H]

Identifier: 238895170

GI number: 238895170

Start: 3051115

End: 3052059

Strand: Reverse

Name: mhpB [H]

Synonym: KP1_3216

Alternate gene names: 238895170

Gene position: 3052059-3051115 (Counterclockwise)

Preceding gene: 238895171

Following gene: 238895169

Centisome position: 58.15

GC content: 65.61

Gene sequence:

>945_bases
ATGCATGCCTATTTGCACTGTCTCTCCCACACCCCGCTGGTGGGATTCGTAGACCCGGAGCAGGCGGTCCTTGATGAAGT
GAACCGGGTGATTGCCGACGCCCGGCGGCGGATCGCTGAATTCGATCCCGAGCTGGTAGTGCTCTTCGCTCCCGATCACT
ACAACGGTTTTTTCTATGACGTGATGCCGCCGTTCTGCCTCGGCGTCGGCGCCACCGCCATCGGCGACTTCGCCAGCGCC
GCCGGCGACCTGCCGGTGCCGACCGAGCTGGCGGAAGCCTGCGCCCACGCCGTGATCAACAGCGGTATCGACCTGGCGGT
GTCGTACAACATGCAGGTGGACCACGGCTTCGCCCAGCCGCTGGAGTTTCTGCTGGGTGGCCTGGACCGCGTACCGGTGC
TGCCGGTGTTTATTAACGGCGTCGCCGCGCCGCTGCCGGGTTTTCAACGCACCCGGCTGCTGGGCGAGGCGATGGGCCGC
TTCCTCAACACCCTCAATAAGCGGGTGCTGATCCTCGGCTCGGGCGGACTCTCGCACCAGCCGCCGGTGCCGGAGCTGGC
GAAGGCCGACGCCCATCTGCGCGATCGCCTGCTCGGCGGCGGAAAACAGCTGCCGCCGGACGAACGCGAACGGCGCCAAC
AGCGGGTGATCAATGCCGCCCGACGGTTTACCGAAGACCCGCACTCGCTCCATCCCCTCAACCCGGTCTGGGACAACCGC
TTTATGAGCCTGCTGGAGCAGGGGCGGCTGAGCGAACTGGACGCGATCGGCAATGACGAGCTGTCGGCGATGGCCGGCAA
ATCGACGCACGAAATTAAGACCTGGGTGGCCGCTTTTGCCGCGCTATCCGCTTTTGGCCGCTGGCGCAGTGAAGGCCGCT
ATTACCGGCCGATCCCCGAGTGGATCGCCGGATTCGGCTCGCTGAGCGCCACCACCGAGATTTGA

Upstream 100 bases:

>100_bases
GCCATCCCGCAAACCCTCGGCGCGCAGCTGACCGCTCTGGCGGAAAAAATGGCCCTCGCAACCGGGGATACCGCCCACGC
TGAAGAGAAGGTGGCCTGAT

Downstream 100 bases:

>100_bases
TTAACCGGGAGACTAACATGAGCTATCAACCGCAAACTGAAGCCGCCACCAGCCGATTCCTCAATGTCGACGAGGGGGGC
CGCACGCTGCGTATCCATAT

Product: 3-(2,3-dihydroxyphenyl)propionate dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MHAYLHCLSHTPLVGFVDPEQAVLDEVNRVIADARRRIAEFDPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFASA
AGDLPVPTELAEACAHAVINSGIDLAVSYNMQVDHGFAQPLEFLLGGLDRVPVLPVFINGVAAPLPGFQRTRLLGEAMGR
FLNTLNKRVLILGSGGLSHQPPVPELAKADAHLRDRLLGGGKQLPPDERERRQQRVINAARRFTEDPHSLHPLNPVWDNR
FMSLLEQGRLSELDAIGNDELSAMAGKSTHEIKTWVAAFAALSAFGRWRSEGRYYRPIPEWIAGFGSLSATTEI

Sequences:

>Translated_314_residues
MHAYLHCLSHTPLVGFVDPEQAVLDEVNRVIADARRRIAEFDPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFASA
AGDLPVPTELAEACAHAVINSGIDLAVSYNMQVDHGFAQPLEFLLGGLDRVPVLPVFINGVAAPLPGFQRTRLLGEAMGR
FLNTLNKRVLILGSGGLSHQPPVPELAKADAHLRDRLLGGGKQLPPDERERRQQRVINAARRFTEDPHSLHPLNPVWDNR
FMSLLEQGRLSELDAIGNDELSAMAGKSTHEIKTWVAAFAALSAFGRWRSEGRYYRPIPEWIAGFGSLSATTEI
>Mature_314_residues
MHAYLHCLSHTPLVGFVDPEQAVLDEVNRVIADARRRIAEFDPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFASA
AGDLPVPTELAEACAHAVINSGIDLAVSYNMQVDHGFAQPLEFLLGGLDRVPVLPVFINGVAAPLPGFQRTRLLGEAMGR
FLNTLNKRVLILGSGGLSHQPPVPELAKADAHLRDRLLGGGKQLPPDERERRQQRVINAARRFTEDPHSLHPLNPVWDNR
FMSLLEQGRLSELDAIGNDELSAMAGKSTHEIKTWVAAFAALSAFGRWRSEGRYYRPIPEWIAGFGSLSATTEI

Specific function: Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3- dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2- hydroxy-6-ketononatrienedioate, respectively [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ligB/mhpB extradiol dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786544, Length=313, Percent_Identity=84.9840255591054, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.16 [H]

Molecular weight: Translated: 34434; Mature: 34434

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYLHCLSHTPLVGFVDPEQAVLDEVNRVIADARRRIAEFDPELVVLFAPDHYNGFFYD
CCHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
VMPPFCLGVGATAIGDFASAAGDLPVPTELAEACAHAVINSGIDLAVSYNMQVDHGFAQP
CCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECEEECCCHHHH
LEFLLGGLDRVPVLPVFINGVAAPLPGFQRTRLLGEAMGRFLNTLNKRVLILGSGGLSHQ
HHHHHCCCCCCCHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PPVPELAKADAHLRDRLLGGGKQLPPDERERRQQRVINAARRFTEDPHSLHPLNPVWDNR
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
FMSLLEQGRLSELDAIGNDELSAMAGKSTHEIKTWVAAFAALSAFGRWRSEGRYYRPIPE
HHHHHHHCCHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHH
WIAGFGSLSATTEI
HHHCCCCCCCCCCC
>Mature Secondary Structure
MHAYLHCLSHTPLVGFVDPEQAVLDEVNRVIADARRRIAEFDPELVVLFAPDHYNGFFYD
CCHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
VMPPFCLGVGATAIGDFASAAGDLPVPTELAEACAHAVINSGIDLAVSYNMQVDHGFAQP
CCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECEEECCCHHHH
LEFLLGGLDRVPVLPVFINGVAAPLPGFQRTRLLGEAMGRFLNTLNKRVLILGSGGLSHQ
HHHHHCCCCCCCHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PPVPELAKADAHLRDRLLGGGKQLPPDERERRQQRVINAARRFTEDPHSLHPLNPVWDNR
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
FMSLLEQGRLSELDAIGNDELSAMAGKSTHEIKTWVAAFAALSAFGRWRSEGRYYRPIPE
HHHHHHHCCHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHH
WIAGFGSLSATTEI
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA