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The map label for this gene is hcaE [H]

Identifier: 238894914

GI number: 238894914

Start: 2800795

End: 2801886

Strand: Reverse

Name: hcaE [H]

Synonym: KP1_2937

Alternate gene names: 238894914

Gene position: 2801886-2800795 (Counterclockwise)

Preceding gene: 238894915

Following gene: 238894913

Centisome position: 53.38

GC content: 57.78

Gene sequence:

>1092_bases
ATGTTATGTCGACGCAAAACCGGAAATAAAAATTCGTTTACCTGTCCGTTTCACGGCTGGACCTTCAGCAATAATGGCAA
ATTATTAAAGGCCAAAGATGAGAGCACTGGCGGCTATCCGCCGAGCTTTAAACAAGACGGCTCCCACGACCTGCAAAAAT
TGCCGCGTTTTCAGTCTTACCGCGGTTTCTTATTCGGTAGCCTCAAGGCCGACGTGCAGCCGCTGGAGGCCTATCTCGGG
GAAACCTGCAAGATTATCGACCTGATCGTCGACCAGGCGCCGGAGGGGCTCGAGGTACTTAAGGGTTCGTCGAGCTATGT
ATATGAGGGCAACTGGAAACTGGGCGCGGAAAACGGCGCCGATGGCTATCACGTCAGCGTGGTGCACTGGAACTACGCTT
CAACCATGTCACGCCGCAACTATGAAGCGGAAGGGACGCACGCGGTGGATGCCAACGGTTGGTCAAAAAGCCTCGGCGGC
GGCTATGGATTTGATAACGGCCATATGCTGTTGTGGACCCGGGCGCTCAACCCGGAAGTGCGCCCGGTCTATGCCCACCG
CGAACGGCTGCAGGCGGAATTTGGCGAACGCCGTGCCGACCAGATGGTCAACGAAACCCGCAACCTGTGCCTGTACCCCA
ATGTCTACCTGATGGATCAGTTCTCGACGCAAATCCGCGTAATCCGCCCCATTGCGGTGGATAAAACCGAAGTCACCATC
TGGTGCTATGCGCCGAAAGGGGAATCCGACCAGGCCAGAGCGCTGCGAATCCGCCAGTACGAGGATTTCTTTAACGTCAG
TGGGATGGGGACGCCGGACGACCTCGAAGAGTTCAGCGCCTGTCAGCGCGGCTATCTCGGCGAAAATCTGCCATGGAGCG
ACCTCAGCCGTGGCGCGCTGCGCTGGGTCGATGGCGCGGATGAGCATGCGCAACATGCCGGCTTTTCGCCGCGGTTAAGC
GGGGTGAAATCGGAGGACGAAGCGCTGTACATCGCCCATCACCACCACTGGCAGACGCTGATGCTGGCGGCCATTGAACA
GGAACAACAGCGTTACGACCAGTCCATTACCCAGCGCGTGGAGGTGGCCTGA

Upstream 100 bases:

>100_bases
TTATTATACCCTGACGCTGGGACGGCAGCCGGTTATTATTACTCGCGATAAAAAGAATTAGCTGCACGCGCTAATTAACA
GCTGCGCTCACCGCGGCGCC

Downstream 100 bases:

>100_bases
TGCTTAACCTCGAACAGGTTCGCCAGTTTCTCTACTACGAAGCCCGTCTGCTGGACGATCGTCAGTGGGACGAATGGCTC
AGCTGCTACAGCCCGCAGGT

Product: 3-phenylpropionate dioxygenase alpha subunit

Products: NA

Alternate protein names: 2-chlorobenzoate 1,2-dioxygenase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MLCRRKTGNKNSFTCPFHGWTFSNNGKLLKAKDESTGGYPPSFKQDGSHDLQKLPRFQSYRGFLFGSLKADVQPLEAYLG
ETCKIIDLIVDQAPEGLEVLKGSSSYVYEGNWKLGAENGADGYHVSVVHWNYASTMSRRNYEAEGTHAVDANGWSKSLGG
GYGFDNGHMLLWTRALNPEVRPVYAHRERLQAEFGERRADQMVNETRNLCLYPNVYLMDQFSTQIRVIRPIAVDKTEVTI
WCYAPKGESDQARALRIRQYEDFFNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGALRWVDGADEHAQHAGFSPRLS
GVKSEDEALYIAHHHHWQTLMLAAIEQEQQRYDQSITQRVEVA

Sequences:

>Translated_363_residues
MLCRRKTGNKNSFTCPFHGWTFSNNGKLLKAKDESTGGYPPSFKQDGSHDLQKLPRFQSYRGFLFGSLKADVQPLEAYLG
ETCKIIDLIVDQAPEGLEVLKGSSSYVYEGNWKLGAENGADGYHVSVVHWNYASTMSRRNYEAEGTHAVDANGWSKSLGG
GYGFDNGHMLLWTRALNPEVRPVYAHRERLQAEFGERRADQMVNETRNLCLYPNVYLMDQFSTQIRVIRPIAVDKTEVTI
WCYAPKGESDQARALRIRQYEDFFNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGALRWVDGADEHAQHAGFSPRLS
GVKSEDEALYIAHHHHWQTLMLAAIEQEQQRYDQSITQRVEVA
>Mature_363_residues
MLCRRKTGNKNSFTCPFHGWTFSNNGKLLKAKDESTGGYPPSFKQDGSHDLQKLPRFQSYRGFLFGSLKADVQPLEAYLG
ETCKIIDLIVDQAPEGLEVLKGSSSYVYEGNWKLGAENGADGYHVSVVHWNYASTMSRRNYEAEGTHAVDANGWSKSLGG
GYGFDNGHMLLWTRALNPEVRPVYAHRERLQAEFGERRADQMVNETRNLCLYPNVYLMDQFSTQIRVIRPIAVDKTEVTI
WCYAPKGESDQARALRIRQYEDFFNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGALRWVDGADEHAQHAGFSPRLS
GVKSEDEALYIAHHHHWQTLMLAAIEQEQQRYDQSITQRVEVA

Specific function: Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=296, Percent_Identity=27.3648648648649, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.13 [H]

Molecular weight: Translated: 41158; Mature: 41158

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCRRKTGNKNSFTCPFHGWTFSNNGKLLKAKDESTGGYPPSFKQDGSHDLQKLPRFQSY
CCCCCCCCCCCCEECCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCHHHC
RGFLFGSLKADVQPLEAYLGETCKIIDLIVDQAPEGLEVLKGSSSYVYEGNWKLGAENGA
CCEEEECHHCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHCCCCCEEEECCEEECCCCCC
DGYHVSVVHWNYASTMSRRNYEAEGTHAVDANGWSKSLGGGYGFDNGHMLLWTRALNPEV
CCEEEEEEEECHHHHHHHCCCCCCCCEEECCCCCHHCCCCCCCCCCCCEEEEEECCCCCC
RPVYAHRERLQAEFGERRADQMVNETRNLCLYPNVYLMDQFSTQIRVIRPIAVDKTEVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCEEEEEEEEECCEEEEE
WCYAPKGESDQARALRIRQYEDFFNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGAL
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCE
RWVDGADEHAQHAGFSPRLSGVKSEDEALYIAHHHHWQTLMLAAIEQEQQRYDQSITQRV
EECCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EVA
CCC
>Mature Secondary Structure
MLCRRKTGNKNSFTCPFHGWTFSNNGKLLKAKDESTGGYPPSFKQDGSHDLQKLPRFQSY
CCCCCCCCCCCCEECCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCHHHC
RGFLFGSLKADVQPLEAYLGETCKIIDLIVDQAPEGLEVLKGSSSYVYEGNWKLGAENGA
CCEEEECHHCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHCCCCCEEEECCEEECCCCCC
DGYHVSVVHWNYASTMSRRNYEAEGTHAVDANGWSKSLGGGYGFDNGHMLLWTRALNPEV
CCEEEEEEEECHHHHHHHCCCCCCCCEEECCCCCHHCCCCCCCCCCCCEEEEEECCCCCC
RPVYAHRERLQAEFGERRADQMVNETRNLCLYPNVYLMDQFSTQIRVIRPIAVDKTEVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCEEEEEEEEECCEEEEE
WCYAPKGESDQARALRIRQYEDFFNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGAL
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCE
RWVDGADEHAQHAGFSPRLSGVKSEDEALYIAHHHHWQTLMLAAIEQEQQRYDQSITQRV
EECCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EVA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7530709; 1370284 [H]