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The map label for this gene is hcaF [H]

Identifier: 238894913

GI number: 238894913

Start: 2800310

End: 2800795

Strand: Reverse

Name: hcaF [H]

Synonym: KP1_2936

Alternate gene names: 238894913

Gene position: 2800795-2800310 (Counterclockwise)

Preceding gene: 238894914

Following gene: 238894912

Centisome position: 53.36

GC content: 57.41

Gene sequence:

>486_bases
ATGCTTAACCTCGAACAGGTTCGCCAGTTTCTCTACTACGAAGCCCGTCTGCTGGACGATCGTCAGTGGGACGAATGGCT
CAGCTGCTACAGCCCGCAGGTGGTCTACTGGATGCCGGCGTGGGGCGACGATGACCAGCTGACCCGCGACCCGCAAAAAG
AGATTTCGCTTATCTACTACCCCAACCGCGAAGGGCTGGAGGATCGCGTCTACCGCATTAAAACCGAGCGCTCCGGGGCC
AGCACGCCAGAGCCGCGGACCACCCATATCATCAGCAACGTTGAGCTGCTGGGCGAAAGCGACGAGGGTCTGGAGGTGCG
CTACAACTGGGTCACCTGGAGCCATCGCTATCAGCACACGGATGCCTATTTTGGCTCTACCTGCTGCACGCTAATCGAAC
AGGACGGCAGACCGCAGATCGTCCGCAAAACCGTGCGCCTGAATAACGATTATATCCGTCAGGTGATTGACGTTTACCAC
ATTTAA

Upstream 100 bases:

>100_bases
TCGCCCATCACCACCACTGGCAGACGCTGATGCTGGCGGCCATTGAACAGGAACAACAGCGTTACGACCAGTCCATTACC
CAGCGCGTGGAGGTGGCCTG

Downstream 100 bases:

>100_bases
GAGGCGCTTATGACCTTCAATATTGCTCTTAATTTTGAGGACGGCGTAACCCGTTTTATTCAGTGCAATGCCGGAGAGAA
AGTCCTGGATGCCGCCTATC

Product: 3-phenylpropionate dioxygenase beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MLNLEQVRQFLYYEARLLDDRQWDEWLSCYSPQVVYWMPAWGDDDQLTRDPQKEISLIYYPNREGLEDRVYRIKTERSGA
STPEPRTTHIISNVELLGESDEGLEVRYNWVTWSHRYQHTDAYFGSTCCTLIEQDGRPQIVRKTVRLNNDYIRQVIDVYH
I

Sequences:

>Translated_161_residues
MLNLEQVRQFLYYEARLLDDRQWDEWLSCYSPQVVYWMPAWGDDDQLTRDPQKEISLIYYPNREGLEDRVYRIKTERSGA
STPEPRTTHIISNVELLGESDEGLEVRYNWVTWSHRYQHTDAYFGSTCCTLIEQDGRPQIVRKTVRLNNDYIRQVIDVYH
I
>Mature_161_residues
MLNLEQVRQFLYYEARLLDDRQWDEWLSCYSPQVVYWMPAWGDDDQLTRDPQKEISLIYYPNREGLEDRVYRIKTERSGA
STPEPRTTHIISNVELLGESDEGLEVRYNWVTWSHRYQHTDAYFGSTCCTLIEQDGRPQIVRKTVRLNNDYIRQVIDVYH
I

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB). The beta subunit may be responsible for the substrate specificity of the enzyme [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017641
- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 19341; Mature: 19341

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLEQVRQFLYYEARLLDDRQWDEWLSCYSPQVVYWMPAWGDDDQLTRDPQKEISLIYY
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEC
PNREGLEDRVYRIKTERSGASTPEPRTTHIISNVELLGESDEGLEVRYNWVTWSHRYQHT
CCCCCCHHHHEEEEECCCCCCCCCCCCEEEECCCEEECCCCCCCEEEEEEEEECCCCCCC
DAYFGSTCCTLIEQDGRPQIVRKTVRLNNDYIRQVIDVYHI
CCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLNLEQVRQFLYYEARLLDDRQWDEWLSCYSPQVVYWMPAWGDDDQLTRDPQKEISLIYY
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEC
PNREGLEDRVYRIKTERSGASTPEPRTTHIISNVELLGESDEGLEVRYNWVTWSHRYQHT
CCCCCCHHHHEEEEECCCCCCCCCCCCEEEECCCEEECCCCCCCEEEEEEEEECCCCCCC
DAYFGSTCCTLIEQDGRPQIVRKTVRLNNDYIRQVIDVYHI
CCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]