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The map label for this gene is hcaE [H]

Identifier: 238894915

GI number: 238894915

Start: 2801926

End: 2802177

Strand: Reverse

Name: hcaE [H]

Synonym: KP1_2938

Alternate gene names: 238894915

Gene position: 2802177-2801926 (Counterclockwise)

Preceding gene: 238894916

Following gene: 238894914

Centisome position: 53.39

GC content: 41.67

Gene sequence:

>252_bases
ATGCAAAAAACGCTCTCGACGCTCAAAGACAAAATCAATAATGCCTTAGTTGTCGACCGTGAAAATCATATTTACCGTTG
CCACCGCTCTATATTTACCGACCAGCAGTTATTTGATTTTGAAATGAAACATATCTTCGAAGGAAATTGGGTTTTTCTTG
CCCATGAAAGCCAGATTGCCGAGCCGGGAGATTATTATACCCTGACGCTGGGACGGCAGCCGGTTATTATTACTCGCGAT
AAAAAGAATTAG

Upstream 100 bases:

>100_bases
TCGGCCCCGGGCACGCTACTGCCCGGGCCAGATATTGCACTCGAAAACGAAGAATTCACCCTGTTTCTTTCATCCTGAAT
CCTGCAGGAGGGACCGACTC

Downstream 100 bases:

>100_bases
CTGCACGCGCTAATTAACAGCTGCGCTCACCGCGGCGCCATGTTATGTCGACGCAAAACCGGAAATAAAAATTCGTTTAC
CTGTCCGTTTCACGGCTGGA

Product: 3-phenylpropionate dioxygenase alpha subunit

Products: 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate; NAD+

Alternate protein names: NA

Number of amino acids: Translated: 83; Mature: 83

Protein sequence:

>83_residues
MQKTLSTLKDKINNALVVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIAEPGDYYTLTLGRQPVIITRD
KKN

Sequences:

>Translated_83_residues
MQKTLSTLKDKINNALVVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIAEPGDYYTLTLGRQPVIITRD
KKN
>Mature_83_residues
MQKTLSTLKDKINNALVVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIAEPGDYYTLTLGRQPVIITRD
KKN

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.12.10

Molecular weight: Translated: 9868; Mature: 9868

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTLSTLKDKINNALVVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIA
CCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHEEECCCEEEEEECCCCC
EPGDYYTLTLGRQPVIITRDKKN
CCCCEEEEEECCCCEEEEECCCC
>Mature Secondary Structure
MQKTLSTLKDKINNALVVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIA
CCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHEEECCCEEEEEECCCCC
EPGDYYTLTLGRQPVIITRDKKN
CCCCEEEEEECCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: benzoate; NADH; H+; O2

Specific reaction: benzoate + NADH + H+ + O2 = 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]