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The map label for this gene is xthA [H]

Identifier: 238894275

GI number: 238894275

Start: 2164559

End: 2165377

Strand: Reverse

Name: xthA [H]

Synonym: KP1_2250

Alternate gene names: 238894275

Gene position: 2165377-2164559 (Counterclockwise)

Preceding gene: 238894282

Following gene: 238894274

Centisome position: 41.26

GC content: 57.63

Gene sequence:

>819_bases
GTGGCAGCGACTATGAAATTCGTCTCTTTTAATATCAACGGCCTGCGTGCCCGCCCACACCAACTGGCCGCCATCGTCGA
AAAACACCAGCCGGACGTGATTGGCCTGCAGGAAACCAAAGTTCACGACGACATGTTCCCTCTCGAAGAGGTCGCCAGGC
TCGGCTATAACGTCTTTTATCACGGGCAGAAAGGCCATTATGGCGTCGCGCTGCTGACCAAAGAGACGCCGATTGCCGTC
CGCCGCGGTTTCCCGGACGATGGCGAAGAGGCTCAGCGCCGCATCATTATGGCGGAGATCCCGTCGCCCTTCGGCAACGT
CACAGTGATTAACGGCTATTTCCCGCAGGGTGAAAGCCGCGATCATGAAACCAAATTCCCGGCCAAAGCGGCGTTCTATC
AGAATCTGCAGAACTATCTGGAGACCGAACTCAACAAAGAGAATCCGGTGCTGATCATGGGCGATATGAACATCAGTCCT
ACCGATCTGGACATCGGTATCGGTGAAGAGAACCGCAAACGCTGGCTGCGCACCGGCAAATGTTCCTTCCTGCCGGAAGA
ACGGGAATGGATGGACCGCCTGCTGGGCTGGGGCCTGGTGGATACCTGGCGTCAGGCTAACCCCGACAATCATGAGCATT
TCTCGTGGTTTGATTACCGCTCCAAAGGCTTCGACGATAACCGTGGGCTGCGCATCGATCTGCTGCTGGCCAGCCAGCCT
CTGGCGCAGCGCTGCGTGGAAACCGGGATCGATTACGAGATCCGCGGGATGGAAAAACCGTCCGACCACGCGCCGGTGTG
GGCCACCTTCCGGCCATAA

Upstream 100 bases:

>100_bases
CCACGGCACGCTCATGCGCAGCGATTGGCAGAATGACGCATCCGGTGGCAGCCAGCCTGGCGAATTTCTGCTACCATCCC
TGCACTATTCACCTTGCACA

Downstream 100 bases:

>100_bases
TTCACGCCCTTCTCTCGCCTCCCGCTGTGCGGGGGGCAGAATTTCCTGAGCAAAATCAACAAAGCGACGGTAAACCATTG
TGACGGTCGTCGGAACAGGA

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAATMKFVSFNINGLRARPHQLAAIVEKHQPDVIGLQETKVHDDMFPLEEVARLGYNVFYHGQKGHYGVALLTKETPIAV
RRGFPDDGEEAQRRIIMAEIPSPFGNVTVINGYFPQGESRDHETKFPAKAAFYQNLQNYLETELNKENPVLIMGDMNISP
TDLDIGIGEENRKRWLRTGKCSFLPEEREWMDRLLGWGLVDTWRQANPDNHEHFSWFDYRSKGFDDNRGLRIDLLLASQP
LAQRCVETGIDYEIRGMEKPSDHAPVWATFRP

Sequences:

>Translated_272_residues
MAATMKFVSFNINGLRARPHQLAAIVEKHQPDVIGLQETKVHDDMFPLEEVARLGYNVFYHGQKGHYGVALLTKETPIAV
RRGFPDDGEEAQRRIIMAEIPSPFGNVTVINGYFPQGESRDHETKFPAKAAFYQNLQNYLETELNKENPVLIMGDMNISP
TDLDIGIGEENRKRWLRTGKCSFLPEEREWMDRLLGWGLVDTWRQANPDNHEHFSWFDYRSKGFDDNRGLRIDLLLASQP
LAQRCVETGIDYEIRGMEKPSDHAPVWATFRP
>Mature_271_residues
AATMKFVSFNINGLRARPHQLAAIVEKHQPDVIGLQETKVHDDMFPLEEVARLGYNVFYHGQKGHYGVALLTKETPIAVR
RGFPDDGEEAQRRIIMAEIPSPFGNVTVINGYFPQGESRDHETKFPAKAAFYQNLQNYLETELNKENPVLIMGDMNISPT
DLDIGIGEENRKRWLRTGKCSFLPEEREWMDRLLGWGLVDTWRQANPDNHEHFSWFDYRSKGFDDNRGLRIDLLLASQPL
AQRCVETGIDYEIRGMEKPSDHAPVWATFRP

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=26.2962962962963, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=26.2962962962963, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=26.2962962962963, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=91.0112359550562, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71989536, Length=267, Percent_Identity=27.3408239700375, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=33.7037037037037, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17136678, Length=270, Percent_Identity=33.7037037037037, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31273; Mature: 31142

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATMKFVSFNINGLRARPHQLAAIVEKHQPDVIGLQETKVHDDMFPLEEVARLGYNVFY
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEECCHHCCCCCCCCCHHHHHHCCCEEEE
HGQKGHYGVALLTKETPIAVRRGFPDDGEEAQRRIIMAEIPSPFGNVTVINGYFPQGESR
ECCCCCEEEEEEECCCCEEEECCCCCCCHHHHHEEEEEECCCCCCCEEEEEEECCCCCCC
DHETKFPAKAAFYQNLQNYLETELNKENPVLIMGDMNISPTDLDIGIGEENRKRWLRTGK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHCCC
CSFLPEEREWMDRLLGWGLVDTWRQANPDNHEHFSWFDYRSKGFDDNRGLRIDLLLASQP
CCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCH
LAQRCVETGIDYEIRGMEKPSDHAPVWATFRP
HHHHHHHCCCCEEEECCCCCCCCCCEEEEECC
>Mature Secondary Structure 
AATMKFVSFNINGLRARPHQLAAIVEKHQPDVIGLQETKVHDDMFPLEEVARLGYNVFY
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEECCHHCCCCCCCCCHHHHHHCCCEEEE
HGQKGHYGVALLTKETPIAVRRGFPDDGEEAQRRIIMAEIPSPFGNVTVINGYFPQGESR
ECCCCCEEEEEEECCCCEEEECCCCCCCHHHHHEEEEEECCCCCCCEEEEEEECCCCCCC
DHETKFPAKAAFYQNLQNYLETELNKENPVLIMGDMNISPTDLDIGIGEENRKRWLRTGK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHCCC
CSFLPEEREWMDRLLGWGLVDTWRQANPDNHEHFSWFDYRSKGFDDNRGLRIDLLLASQP
CCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCH
LAQRCVETGIDYEIRGMEKPSDHAPVWATFRP
HHHHHHHCCCCEEEECCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]