Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 238892338

GI number: 238892338

Start: 87577

End: 89844

Strand: Reverse

Name: priA [H]

Synonym: KP1_0092

Alternate gene names: 238892338

Gene position: 89844-87577 (Counterclockwise)

Preceding gene: 238892340

Following gene: 238892337

Centisome position: 1.71

GC content: 62.83

Gene sequence:

>2268_bases
ATGCAAACTGATCCGCGCCGCCTTATCGTAATGTGTATACTATCCCGCCAGATTTCAAGTCAGGAAGATGACATGTCCGT
CGCCCACGTTGCCCTACCCGTTCCGCTACCGCGCACTTTTGACTACCTGCTGCCTGAAGGCATGGCGGTCAAAGCGGGCT
GTCGGGTGCGGGTGCCGTTCGGCAAACAGGAGCGGATCGGCATTGTCGCGGCGGTGAGCGAGCGCAGCGAACTGCCGCTG
GATGAGCTAAAACCGGTCGCCGAAGCGCTGGACGACGAGCCGGTCTTCTCCACGACTGTCTGGCGACTGCTGATGTGGGC
GGCTGAGTATTACCATCACCCGATCGGCGATGTGCTGTTCCATGCATTACCCGTTATGCTGCGCCAGGGCAAACCCGCCA
GCGCCACGCCGCTGTGGTACTGGTTCGCCACCGAGCAGGGGCAGGTTGTCGACCTTAACGGCCTGAAACGCTCCCGGAAG
CAGCAGCAGGCGCTGGCGGCGCTGCGCCAGGGTAAAATCTGGCGTCATCAGGTTGGCGAACTGGAATTCAATGAAGCGGC
GCTGCAGGCGCTGCGCGGTAAAGGGCTGGCGGAGCTGGCCTGCGAAGCGCCCGCCCTCACCGACTGGCGCAGCGCGTATT
CGGTTGCCGGCGAACGCCTGCGCCTGAATACCGAGCAGGCCACCGCCGTCGGGGCGATCCACAGCGCCGCCGATCGTTTC
TCCGCCTGGCTGCTGGCGGGTATTACCGGCTCCGGGAAAACGGAAGTCTATTTGAGCGTGCTGGAGAATGTGCTGGCGCA
GGGGCGCCAGGCGCTGGTGATGGTGCCGGAGATCGGCCTGACGCCGCAAACCATCGCCCGATTTCGCCAGCGCTTTAACG
CGCCGGTGGAAGTGCTGCACTCCGGGCTCAATGACAGCGAACGTCTCTCGGCCTGGCTGAAGGCGAAAAATGGCGAAGCG
GCCATTGTGATCGGCACCCGCTCCTCGCTGTTTACCCCATTTAAAGATCTCGGCGTCATCGTCATCGACGAAGAGCATGA
CAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTACCATGCCCGCGACCTGGCGGTATGGCGCGCCCACAGCGAGCAGATCC
CGATAATTCTCGGCTCGGCGACGCCGGCGCTGGAGACTCTGCATAACGTCCGCCAGGGTAAATACCGCCAGTTAACCTTA
AGCAAACGCGCGGGCAATGCGCGCCCGGCCCAGCAGCACGTGCTCGATCTCAAAGGGCTACCGCTGCAGGCGGGCCTGTC
TCCCGCGCTGATTAGCCGTATGCGCCAGCACCTGCAAGCGGATAACCAGGTGATCCTGTTTCTTAACCGCCGCGGCTTTG
CGCCAGCGCTGCTGTGCCACGATTGCGGCTGGATAGCGGAATGCCCGCGCTGCGACAGTTACTATACGTTGCACCAGGCG
CAGCACCATCTGCGCTGCCACCATTGCGACAGCCAGCGCCCGATCCCGCGCCAGTGTCCTTCCTGCGGCTCCACGCATCT
GGTGCCGGTAGGGATCGGCACCGAGCAGCTGGAGCAGGCCCTGGCGCCGCTGTTCCCTGAGGTGCCTATCTCGCGTATCG
ACCGCGATACCACCAGCCGCAAAGGAGCGCTGGAGGAACATCTCGCCGCCGTCCATCGCGGCGGGGCGCGTATTCTGATT
GGCACCCAAATGCTGGCCAAAGGGCACCACTTCCCGGACGTCACCCTCGTCTCCCTGCTGGATGTCGACGGGGCGCTGTT
TTCCGCTGATTTCCGCTCCGCAGAGCGTTTCGCTCAGCTTTATACCCAGGTCTCCGGACGAGCGGGCCGGGCAGGAAAAC
AGGGCGAGGTCATTCTTCAAACCCACCATCCGGAACACCCTCTACTGCAGACCCTGCTGTATAAAGGCTACGACGCCTTT
GCCGAACAGGCGCTGGCAGAGCGGCAGACGATGCAGCTGCCGCCGTGGACCAGCCATGTGCTGATTCGTGCGGAAGACCA
TAACAACCAGCAGGCGCCGCTGTTTTTACAACAGCTGCGCAATCTGCTGCAGGCCAGCCCGCTGGCCGACGAAAAGCTAT
GGGTGCTCGGTCCGGTTCCGGCGCTGGCGCCAAAGCGCGGCGGCCGCTGGCGCTGGCAAATATTGCTGCAGCATCCTTCG
CGGGTGCGTCTGCAACATATCGTTAGCGGTACGCTGGCGCTGATCAATACGCTTCCGGAGGCGAGAAAAGTGAAGTGGGT
GCTGGATGTCGATCCGATTGAAGGATAA

Upstream 100 bases:

>100_bases
TTCTACGCTGCTGACAGCGTGAAGCACGCGGTAATGGTCAATCTTCAATGCGATATAAAGCGCATCAAAGGCGGCGAATC
ATACAGAATTTGACCAGCAT

Downstream 100 bases:

>100_bases
GCCTTCTCTTGCGAGCCGGATCGAAAAATTTAACAAGCATCACGTTTTTCATGAAAATTCTGTAACTCACTGCCGCGACT
ATCTGATAAAAATGATGCAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MQTDPRRLIVMCILSRQISSQEDDMSVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQERIGIVAAVSERSELPL
DELKPVAEALDDEPVFSTTVWRLLMWAAEYYHHPIGDVLFHALPVMLRQGKPASATPLWYWFATEQGQVVDLNGLKRSRK
QQQALAALRQGKIWRHQVGELEFNEAALQALRGKGLAELACEAPALTDWRSAYSVAGERLRLNTEQATAVGAIHSAADRF
SAWLLAGITGSGKTEVYLSVLENVLAQGRQALVMVPEIGLTPQTIARFRQRFNAPVEVLHSGLNDSERLSAWLKAKNGEA
AIVIGTRSSLFTPFKDLGVIVIDEEHDSSYKQQEGWRYHARDLAVWRAHSEQIPIILGSATPALETLHNVRQGKYRQLTL
SKRAGNARPAQQHVLDLKGLPLQAGLSPALISRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDSYYTLHQA
QHHLRCHHCDSQRPIPRQCPSCGSTHLVPVGIGTEQLEQALAPLFPEVPISRIDRDTTSRKGALEEHLAAVHRGGARILI
GTQMLAKGHHFPDVTLVSLLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVILQTHHPEHPLLQTLLYKGYDAF
AEQALAERQTMQLPPWTSHVLIRAEDHNNQQAPLFLQQLRNLLQASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPS
RVRLQHIVSGTLALINTLPEARKVKWVLDVDPIEG

Sequences:

>Translated_755_residues
MQTDPRRLIVMCILSRQISSQEDDMSVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQERIGIVAAVSERSELPL
DELKPVAEALDDEPVFSTTVWRLLMWAAEYYHHPIGDVLFHALPVMLRQGKPASATPLWYWFATEQGQVVDLNGLKRSRK
QQQALAALRQGKIWRHQVGELEFNEAALQALRGKGLAELACEAPALTDWRSAYSVAGERLRLNTEQATAVGAIHSAADRF
SAWLLAGITGSGKTEVYLSVLENVLAQGRQALVMVPEIGLTPQTIARFRQRFNAPVEVLHSGLNDSERLSAWLKAKNGEA
AIVIGTRSSLFTPFKDLGVIVIDEEHDSSYKQQEGWRYHARDLAVWRAHSEQIPIILGSATPALETLHNVRQGKYRQLTL
SKRAGNARPAQQHVLDLKGLPLQAGLSPALISRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDSYYTLHQA
QHHLRCHHCDSQRPIPRQCPSCGSTHLVPVGIGTEQLEQALAPLFPEVPISRIDRDTTSRKGALEEHLAAVHRGGARILI
GTQMLAKGHHFPDVTLVSLLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVILQTHHPEHPLLQTLLYKGYDAF
AEQALAERQTMQLPPWTSHVLIRAEDHNNQQAPLFLQQLRNLLQASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPS
RVRLQHIVSGTLALINTLPEARKVKWVLDVDPIEG
>Mature_755_residues
MQTDPRRLIVMCILSRQISSQEDDMSVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQERIGIVAAVSERSELPL
DELKPVAEALDDEPVFSTTVWRLLMWAAEYYHHPIGDVLFHALPVMLRQGKPASATPLWYWFATEQGQVVDLNGLKRSRK
QQQALAALRQGKIWRHQVGELEFNEAALQALRGKGLAELACEAPALTDWRSAYSVAGERLRLNTEQATAVGAIHSAADRF
SAWLLAGITGSGKTEVYLSVLENVLAQGRQALVMVPEIGLTPQTIARFRQRFNAPVEVLHSGLNDSERLSAWLKAKNGEA
AIVIGTRSSLFTPFKDLGVIVIDEEHDSSYKQQEGWRYHARDLAVWRAHSEQIPIILGSATPALETLHNVRQGKYRQLTL
SKRAGNARPAQQHVLDLKGLPLQAGLSPALISRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDSYYTLHQA
QHHLRCHHCDSQRPIPRQCPSCGSTHLVPVGIGTEQLEQALAPLFPEVPISRIDRDTTSRKGALEEHLAAVHRGGARILI
GTQMLAKGHHFPDVTLVSLLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVILQTHHPEHPLLQTLLYKGYDAF
AEQALAERQTMQLPPWTSHVLIRAEDHNNQQAPLFLQQLRNLLQASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPS
RVRLQHIVSGTLALINTLPEARKVKWVLDVDPIEG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=732, Percent_Identity=88.1147540983607, Blast_Score=1344, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84122; Mature: 84122

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTDPRRLIVMCILSRQISSQEDDMSVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPF
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCCCCEEECCCEEECCC
GKQERIGIVAAVSERSELPLDELKPVAEALDDEPVFSTTVWRLLMWAAEYYHHPIGDVLF
CCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
HALPVMLRQGKPASATPLWYWFATEQGQVVDLNGLKRSRKQQQALAALRQGKIWRHQVGE
HHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCC
LEFNEAALQALRGKGLAELACEAPALTDWRSAYSVAGERLRLNTEQATAVGAIHSAADRF
CCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHH
SAWLLAGITGSGKTEVYLSVLENVLAQGRQALVMVPEIGLTPQTIARFRQRFNAPVEVLH
HHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHH
SGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKDLGVIVIDEEHDSSYKQQEGWRYHA
CCCCCHHHHHHHHCCCCCCEEEEEECCHHHCCCHHHCCEEEEECCCCCCHHHHCCCEEHH
RDLAVWRAHSEQIPIILGSATPALETLHNVRQGKYRQLTLSKRAGNARPAQQHVLDLKGL
HHHHEEECCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCCC
PLQAGLSPALISRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDSYYTLHQA
CHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCHHHHHHH
QHHLRCHHCDSQRPIPRQCPSCGSTHLVPVGIGTEQLEQALAPLFPEVPISRIDRDTTSR
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHCCCCCCCC
KGALEEHLAAVHRGGARILIGTQMLAKGHHFPDVTLVSLLDVDGALFSADFRSAERFAQL
CCHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
YTQVSGRAGRAGKQGEVILQTHHPEHPLLQTLLYKGYDAFAEQALAERQTMQLPPWTSHV
HHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEE
LIRAEDHNNQQAPLFLQQLRNLLQASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPS
EEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCC
RVRLQHIVSGTLALINTLPEARKVKWVLDVDPIEG
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MQTDPRRLIVMCILSRQISSQEDDMSVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPF
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCCCCEEECCCEEECCC
GKQERIGIVAAVSERSELPLDELKPVAEALDDEPVFSTTVWRLLMWAAEYYHHPIGDVLF
CCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
HALPVMLRQGKPASATPLWYWFATEQGQVVDLNGLKRSRKQQQALAALRQGKIWRHQVGE
HHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCC
LEFNEAALQALRGKGLAELACEAPALTDWRSAYSVAGERLRLNTEQATAVGAIHSAADRF
CCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHH
SAWLLAGITGSGKTEVYLSVLENVLAQGRQALVMVPEIGLTPQTIARFRQRFNAPVEVLH
HHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHH
SGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKDLGVIVIDEEHDSSYKQQEGWRYHA
CCCCCHHHHHHHHCCCCCCEEEEEECCHHHCCCHHHCCEEEEECCCCCCHHHHCCCEEHH
RDLAVWRAHSEQIPIILGSATPALETLHNVRQGKYRQLTLSKRAGNARPAQQHVLDLKGL
HHHHEEECCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCCC
PLQAGLSPALISRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDSYYTLHQA
CHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCHHHHHHH
QHHLRCHHCDSQRPIPRQCPSCGSTHLVPVGIGTEQLEQALAPLFPEVPISRIDRDTTSR
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHCCCCCCCC
KGALEEHLAAVHRGGARILIGTQMLAKGHHFPDVTLVSLLDVDGALFSADFRSAERFAQL
CCHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
YTQVSGRAGRAGKQGEVILQTHHPEHPLLQTLLYKGYDAFAEQALAERQTMQLPPWTSHV
HHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEE
LIRAEDHNNQQAPLFLQQLRNLLQASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPS
EEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCC
RVRLQHIVSGTLALINTLPEARKVKWVLDVDPIEG
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]