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The map label for this gene is gatB [H]

Identifier: 238028926

GI number: 238028926

Start: 3853397

End: 3854869

Strand: Reverse

Name: gatB [H]

Synonym: bglu_1g33910

Alternate gene names: 238028926

Gene position: 3854869-3853397 (Counterclockwise)

Preceding gene: 238028927

Following gene: 238028925

Centisome position: 98.68

GC content: 66.94

Gene sequence:

>1473_bases
ATGACGCAATGGGAAGTCGTTATCGGTCTCGAGACGCACGCGCAGCTGTCGACCGCCTCGAAGATCTTCTCGGGCGCCTC
GACGCAGTTCGGCGCCGAGCCCAACACCCAGGCCTGCGCGGTCGATCTCGCGCTGCCCGGCGTGCTGCCGCTCTTGAACC
GCGGCGCGGTGGAGCGCGCGATCCGGCTGGGCCTCGCGGTCGACGCGACGGTGGCGCCGCGCAGCATCTTCGCGCGCAAG
AACTACTTCTATCCGGATCTGCCCAAGGGCTATCAGATCAGCCAGTACGAGATTCCGGTCGTGCAGGGCGGCCGCATCAC
GATCCAGGTGCCGGCCAACGAGAAGGCCGGCAAGGCGGCCTACGCGAAGACCATCCATCTCACGCGCGCGCACCTCGAGG
AAGACGCCGGCAAGTCGCTGCACGAGGATTTCGCCGGCATGACCGGCATCGACCTGAACCGCGCCGGCACGCCGCTGCTC
GAGATCGTCACCGAGCCCGAGATGCGCAGCGCGGCCGAGGCCGTGGCCTACGCGAAGGCGCTGCATGCGCTGGTGGTCTG
GCTCGGCATCTGCGACGGCAACATGCAGGAAGGCTCGTTCCGCTGCGACGCGAACGTCTCGGTGCGGCCGCTCGGCCAGG
AGACGTTCGGCACGCGCGCCGAGATCAAGAACCTGAACTCGTTCCGCTTCCTCGAGGACGCGATCAACTACGAGGTGCGG
CGCCAGATCGAGCTGATCAAGGACGGCGGCCAGGTGGTGCAGGAAACGCGCCTGTACGATCCCGACAAGCGCGAGACGCG
CTCGATGCGCAGCAAGGAAGACGCGCAGGACTACCGCTATTTCCCCGATCCCGACCTGATGCCGGTGGTGATCGATCCGG
AATGGGTCGAGCGCGTGCGCGGCGAGATGCCCGAACTGCCGGCCGCGATGCAGCAGCGCTTCGCCGACCAGTACGGCGTG
ACGCCCTATGACGCGGTGGTGCTGACCTCGAGCAAGGCGATGGCCGCCTATTTCGAGGCGGTGGTCGCCAAGGCCGGCGC
GGCGAACGCGAAGGCCGCCGCCAACTGGCTGATGGGCGACGTATCGTCCCTGTTGAACCGTGAAGGCATCGAGATCGACG
CGTGTCCGGTATCGGCCGCCCAGCTCGCGCTGGTGCTGCAGCGCATCGCCGACGGCACCATCTCGAACAAGATCGCCAAG
GAAATCTTCCAGGCGATCTGGGACGAGCAAGCCACCGACGAGGCCGCAGCCGACCGCATCATCGAGGCGAAGGGCCTCAA
GCAGATCTCCGACACCGGCGAGCTGGAGGCGATCATCGACGCGGTGCTGGCCGCCAACGCGAAGTCGGTCGAGGAGTTCC
GCGCCGGCAAGGAGAAGGCGTTCAACGCGCTGGTCGGCCAGGCGATGAAGGCGACCAAGGGCAAGGCGAACCCGGCCCAG
GTCAACGAACTGCTGAAGAAGAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
CATCGGCAACTATTTCAACGAAGCCCGGATGCTGCAGGTCGCCGACGCGTTCCAGCGCGCGACCGACTGGCACAAGCAAG
TTCCGGCGGGGGTATAAGCA

Downstream 100 bases:

>100_bases
CGGCGGGCCCGAGCGGCGCCGAGCGGCGGCCGCTCGCGTGGGTCGCAGCCGCCGGCCGGCCGCTTGCGTTATGCTCGACG
GCTTGGCGCCGCAGCGGCGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTQWEVVIGLETHAQLSTASKIFSGASTQFGAEPNTQACAVDLALPGVLPLLNRGAVERAIRLGLAVDATVAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGRITIQVPANEKAGKAAYAKTIHLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPLGQETFGTRAEIKNLNSFRFLEDAINYEVR
RQIELIKDGGQVVQETRLYDPDKRETRSMRSKEDAQDYRYFPDPDLMPVVIDPEWVERVRGEMPELPAAMQQRFADQYGV
TPYDAVVLTSSKAMAAYFEAVVAKAGAANAKAAANWLMGDVSSLLNREGIEIDACPVSAAQLALVLQRIADGTISNKIAK
EIFQAIWDEQATDEAAADRIIEAKGLKQISDTGELEAIIDAVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQ
VNELLKKKLG

Sequences:

>Translated_490_residues
MTQWEVVIGLETHAQLSTASKIFSGASTQFGAEPNTQACAVDLALPGVLPLLNRGAVERAIRLGLAVDATVAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGRITIQVPANEKAGKAAYAKTIHLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPLGQETFGTRAEIKNLNSFRFLEDAINYEVR
RQIELIKDGGQVVQETRLYDPDKRETRSMRSKEDAQDYRYFPDPDLMPVVIDPEWVERVRGEMPELPAAMQQRFADQYGV
TPYDAVVLTSSKAMAAYFEAVVAKAGAANAKAAANWLMGDVSSLLNREGIEIDACPVSAAQLALVLQRIADGTISNKIAK
EIFQAIWDEQATDEAAADRIIEAKGLKQISDTGELEAIIDAVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQ
VNELLKKKLG
>Mature_489_residues
TQWEVVIGLETHAQLSTASKIFSGASTQFGAEPNTQACAVDLALPGVLPLLNRGAVERAIRLGLAVDATVAPRSIFARKN
YFYPDLPKGYQISQYEIPVVQGGRITIQVPANEKAGKAAYAKTIHLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLE
IVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPLGQETFGTRAEIKNLNSFRFLEDAINYEVRR
QIELIKDGGQVVQETRLYDPDKRETRSMRSKEDAQDYRYFPDPDLMPVVIDPEWVERVRGEMPELPAAMQQRFADQYGVT
PYDAVVLTSSKAMAAYFEAVVAKAGAANAKAAANWLMGDVSSLLNREGIEIDACPVSAAQLALVLQRIADGTISNKIAKE
IFQAIWDEQATDEAAADRIIEAKGLKQISDTGELEAIIDAVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQV
NELLKKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=37.6, Blast_Score=355, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=466, Percent_Identity=32.1888412017167, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=32.1223709369025, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=35.6136820925553, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53339; Mature: 53208

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQWEVVIGLETHAQLSTASKIFSGASTQFGAEPNTQACAVDLALPGVLPLLNRGAVERA
CCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHCCHHHHHCCHHHHHH
IRLGLAVDATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGRITIQVPANEKAGKAA
HHHCCEECCCCCCHHHHHHCCCCCCCCCCCCCCCEEECEEEECCEEEEEECCCCCCCCHH
YAKTIHLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAKA
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LHALVVWLGICDGNMQEGSFRCDANVSVRPLGQETFGTRAEIKNLNSFRFLEDAINYEVR
HHHHHHHHHHCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHHHHHHHH
RQIELIKDGGQVVQETRLYDPDKRETRSMRSKEDAQDYRYFPDPDLMPVVIDPEWVERVR
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHC
GEMPELPAAMQQRFADQYGVTPYDAVVLTSSKAMAAYFEAVVAKAGAANAKAAANWLMGD
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VSSLLNREGIEIDACPVSAAQLALVLQRIADGTISNKIAKEIFQAIWDEQATDEAAADRI
HHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IEAKGLKQISDTGELEAIIDAVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQ
HHHHCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHH
VNELLKKKLG
HHHHHHHHCC
>Mature Secondary Structure 
TQWEVVIGLETHAQLSTASKIFSGASTQFGAEPNTQACAVDLALPGVLPLLNRGAVERA
CCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHCCHHHHHCCHHHHHH
IRLGLAVDATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGRITIQVPANEKAGKAA
HHHCCEECCCCCCHHHHHHCCCCCCCCCCCCCCCEEECEEEECCEEEEEECCCCCCCCHH
YAKTIHLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAKA
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LHALVVWLGICDGNMQEGSFRCDANVSVRPLGQETFGTRAEIKNLNSFRFLEDAINYEVR
HHHHHHHHHHCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHHHHHHHH
RQIELIKDGGQVVQETRLYDPDKRETRSMRSKEDAQDYRYFPDPDLMPVVIDPEWVERVR
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHC
GEMPELPAAMQQRFADQYGVTPYDAVVLTSSKAMAAYFEAVVAKAGAANAKAAANWLMGD
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VSSLLNREGIEIDACPVSAAQLALVLQRIADGTISNKIAKEIFQAIWDEQATDEAAADRI
HHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IEAKGLKQISDTGELEAIIDAVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQ
HHHHCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHH
VNELLKKKLG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA