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The map label for this gene is gatA [H]

Identifier: 238028927

GI number: 238028927

Start: 3854873

End: 3856363

Strand: Reverse

Name: gatA [H]

Synonym: bglu_1g33920

Alternate gene names: 238028927

Gene position: 3856363-3854873 (Counterclockwise)

Preceding gene: 238028928

Following gene: 238028926

Centisome position: 98.72

GC content: 71.9

Gene sequence:

>1491_bases
ATGCATGCAAACAGCCTGACCGAACTGCGCGCCGCGCTCGACGCCGGCCAAACCTCGGCCGTCGAGCTCGCGCAGCTCTA
CCTGCAACGCATCGACGCGGCGCGCGAGCTCAATGCGTTCGTCCATGTCGACGCCGAGCTCACGCTCGCCCAGGCGCGCG
AGGCCGACGCCGCGCGCGCGGCCGGCCGTGCCGGCCCGCTCGCCGGCCTGCCGGTCGCGCACAAGGACGTGTTCGTCACG
CGCGGCTGGCGCTCCACCGCCGGCTCGAAGATGCTCGCGAACTACACCAGCCCGTTCGACGCGACGGTGGTCGAGCGGCT
CGCCGCGGCCGGCATGGTCACGCTCGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCCAACGAGAACTCGGCGT
TCGGCGCCGTGAAGAACCCGTGGGACCCCCGGGCCGTGCCGGGCGGCAGCTCGGGCGGCAGCGCGGCGGCGGTGGCCGCG
CGCCTCGCGCCCGCCGCCACCGGCACCGACACCGGCGGCTCGATCCGCCAGCCGGCCTCGTTCGCCGGCGTGACCGGCAT
CAAGCCGACCTACGGCCGGGTGTCGCGCTACGGCATGATCGCGTTCGCTTCCTCGCTCGACCAGGGCGGCCCGATGGCGC
AGAGCGCGGCCGACTGCGCGCTGCTGCTCGACGCGATGGCCGGCTTCGACACGCGCGACTCCACCAGCCTGGAGCGCGAG
GGCGAGGCGTTCGGCCGCCACCTGGGCGCGCCGTGGACGCCCGACGCGCCGGCCGGCAAGCCGCTGGCGGGCCTGCGCAT
CGGCCTGCCGGACGAGTATTTCGGTGCCGGGCTGGCCGACGACGTGCGCGCGGCGATCGACGCGGCGCTCAAGCAGTACG
AGGCGCTCGGCGCCACGCTGGTGCCGGTGTCGCTGCCGAAGACGGAGCTGTCGATCCCGGTCTACTACGTGATCGCGCCG
GCCGAGGCCTCGTCGAACCTGTCGCGCTTCGACGGCGTGCGCTACGGCCATCGCGCGGCCGAGTACCGCGACCTGCTCGA
CATGTACAAGAAGTCGCGCGCCGAGGGCTTCGGCCCGGAAGTGAAGCGCCGCATCCTGGTGGGCACCTACGTGCTCTCGC
ATGGCTACTACGACGCGTACTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCGCAGGATTTCCAGAACGCGTTCGAG
CAGTGCGACGTGATCATGGGGCCGGCCTCGCCCACCGTGGCCTGGGATCTCGGTGCCAAGGGCGACGACCCGCTGCAGAT
GTACCTGGCCGACATCTACACGCTGTCGGTCAGCCTGGCGGGCCTGCCCGGCATGAGCGTGCCGTGCGGCTTCGGCGCCG
GCGCGAACGCGCGGCGCCCGGTCGGCCTGCAGATCATCGGCAACTATTTCAACGAAGCCCGGATGCTGCAGGTCGCCGAC
GCGTTCCAGCGCGCGACCGACTGGCACAAGCAAGTTCCGGCGGGGGTATAA

Upstream 100 bases:

>100_bases
TCGAGTAAGCGCGCTTCGGAGCCGCATGGCCGGCCCGCCGTCGTCTGCCCGGGCGCGCGGCCGTGCCGCGCATCCACAAG
AATCCAGGAATCCTCCAGCA

Downstream 100 bases:

>100_bases
GCAATGACGCAATGGGAAGTCGTTATCGGTCTCGAGACGCACGCGCAGCTGTCGACCGCCTCGAAGATCTTCTCGGGCGC
CTCGACGCAGTTCGGCGCCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHANSLTELRAALDAGQTSAVELAQLYLQRIDAARELNAFVHVDAELTLAQAREADAARAAGRAGPLAGLPVAHKDVFVT
RGWRSTAGSKMLANYTSPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDPRAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSAADCALLLDAMAGFDTRDSTSLERE
GEAFGRHLGAPWTPDAPAGKPLAGLRIGLPDEYFGAGLADDVRAAIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNAFE
QCDVIMGPASPTVAWDLGAKGDDPLQMYLADIYTLSVSLAGLPGMSVPCGFGAGANARRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV

Sequences:

>Translated_496_residues
MHANSLTELRAALDAGQTSAVELAQLYLQRIDAARELNAFVHVDAELTLAQAREADAARAAGRAGPLAGLPVAHKDVFVT
RGWRSTAGSKMLANYTSPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDPRAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSAADCALLLDAMAGFDTRDSTSLERE
GEAFGRHLGAPWTPDAPAGKPLAGLRIGLPDEYFGAGLADDVRAAIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNAFE
QCDVIMGPASPTVAWDLGAKGDDPLQMYLADIYTLSVSLAGLPGMSVPCGFGAGANARRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV
>Mature_496_residues
MHANSLTELRAALDAGQTSAVELAQLYLQRIDAARELNAFVHVDAELTLAQAREADAARAAGRAGPLAGLPVAHKDVFVT
RGWRSTAGSKMLANYTSPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDPRAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSAADCALLLDAMAGFDTRDSTSLERE
GEAFGRHLGAPWTPDAPAGKPLAGLRIGLPDEYFGAGLADDVRAAIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNAFE
QCDVIMGPASPTVAWDLGAKGDDPLQMYLADIYTLSVSLAGLPGMSVPCGFGAGANARRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=512, Percent_Identity=39.6484375, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=25.2032520325203, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI166795287, Length=156, Percent_Identity=37.8205128205128, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=453, Percent_Identity=37.0860927152318, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=304, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=32.8125, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=324, Percent_Identity=27.1604938271605, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=229, Percent_Identity=31.4410480349345, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=199, Percent_Identity=28.1407035175879, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=447, Percent_Identity=34.2281879194631, Blast_Score=243, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=470, Percent_Identity=26.1702127659574, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=195, Percent_Identity=30.2564102564103, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=39.7148676171079, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24652985, Length=504, Percent_Identity=24.8015873015873, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922090, Length=504, Percent_Identity=24.8015873015873, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24652981, Length=504, Percent_Identity=24.8015873015873, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24652983, Length=504, Percent_Identity=24.8015873015873, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078093, Length=532, Percent_Identity=27.2556390977444, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24644968, Length=242, Percent_Identity=33.8842975206612, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=31.9148936170213, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=31.9148936170213, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=31.9148936170213, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=238, Percent_Identity=31.0924369747899, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24648441, Length=167, Percent_Identity=35.3293413173653, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648439, Length=167, Percent_Identity=35.3293413173653, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52391; Mature: 52391

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHANSLTELRAALDAGQTSAVELAQLYLQRIDAARELNAFVHVDAELTLAQAREADAARA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHH
AGRAGPLAGLPVAHKDVFVTRGWRSTAGSKMLANYTSPFDATVVERLAAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGAVKNPWDPRAVPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSAADCALLLDAMAGFDTRDSTSLERE
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
GEAFGRHLGAPWTPDAPAGKPLAGLRIGLPDEYFGAGLADDVRAAIDAALKQYEALGATL
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNAFEQCDVIMGPASPTVAWDLGAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEECCCC
GDDPLQMYLADIYTLSVSLAGLPGMSVPCGFGAGANARRPVGLQIIGNYFNEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AFQRATDWHKQVPAGV
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHANSLTELRAALDAGQTSAVELAQLYLQRIDAARELNAFVHVDAELTLAQAREADAARA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHH
AGRAGPLAGLPVAHKDVFVTRGWRSTAGSKMLANYTSPFDATVVERLAAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGAVKNPWDPRAVPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSAADCALLLDAMAGFDTRDSTSLERE
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
GEAFGRHLGAPWTPDAPAGKPLAGLRIGLPDEYFGAGLADDVRAAIDAALKQYEALGATL
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNAFEQCDVIMGPASPTVAWDLGAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEECCCC
GDDPLQMYLADIYTLSVSLAGLPGMSVPCGFGAGANARRPVGLQIIGNYFNEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AFQRATDWHKQVPAGV
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA