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The map label for this gene is bioB [H]

Identifier: 238028782

GI number: 238028782

Start: 3686975

End: 3688054

Strand: Direct

Name: bioB [H]

Synonym: bglu_1g32460

Alternate gene names: 238028782

Gene position: 3686975-3688054 (Clockwise)

Preceding gene: 238028781

Following gene: 238028789

Centisome position: 94.38

GC content: 69.07

Gene sequence:

>1080_bases
ATGATCCAAGCCCAGACCGTGACTCCGGACGCGACGACGGCACCCGAAGCAGTGCCCGTCGGCATGCCGAGCGCGCCGCG
CTGGCGCGTCGCCGACGTCGTCAAGCTCTACGAACTGCCGTTCAACGACCTGCTGTTCCGCGCCCAGCAGGTGCATCGCG
AGCACTTCGACGCCAACGCGATCCAGCTCTCCACGCTGCTGTCGATCAAGACCGGCGGCTGCGAGGAGGATTGCGGCTAC
TGCTCGCAGTCCTCGCACCACGAGACCGAGCTGAAGGCGAGCAAGCTGATGGACGTGGACGCCGTGCTCGACGCCGCGCG
CGCGGCCAAGGCCAACGGCGCGACGCGCTTCTGCATGGGCGCCGCGTGGCGCAGCCCGAAGGAGCGCCATATCGAGCCGC
TCGCCGAGATGATCCGCGGCGTGAAGGAGATGGGCCTCGAGACCTGCATGACGCTCGGCATGCTCGACGACGGCCAGGCG
CAGGCCTTGTCCGAGGCCGGCCTCGACTACTACAACCACAACCTCGACACCTCGCCCGAGTTCTACGGCCAGGTGATCTC
GACGCGCACCTACCAGGACCGGCTCGACACGCTCGAGCGCGTGCGCGACGCCGGCATCAACGTCTGCTGCGGCGGCATCA
TCGGCATGGGCGAATCGCGCCGCGAGCGCGCCGGGCTCATCACCCAGCTCGCGAACATGAACCCGTATCCGGAATCGGTG
CCGATCAACAACCTCGTCGCGATCAAGGGCACGCCGCTCGAGAACACCGCGCCGCTCGATCCGCTCGAATTCGTGCGCAC
CATCGCCGTGGCGCGCATCACCATGCCGAAGGCGATGGTGCGGCTGTCCGCCGGGCGCGAGCAGCTCGACGACGCGATGC
AGACGCTGTGCTTCCTGGCCGGCGCAAACTCGATGTTCTACGGCGACCAGCTGCTGACCACCAGCAACCCGCAGGCGCAG
AAGGACCGCGACCTGCTCGCGCGGCTCGGCATGCGCGCCGAGGCGGCCCAGCAGATGCCGGTGGACGCCGCCGCCGACGC
GGTGAAGTGCGCGCACGCGCCGGGGCCGGTGGCGAACTGA

Upstream 100 bases:

>100_bases
AACTGGCCGCGGGCTCGCTCGACATCGAACGGCTCGTCGCGGCGCTCGCCGCCGCGCGGCCGTGATACCCCGTTCCCCGC
TTCCTATCGAGGACCCCACC

Downstream 100 bases:

>100_bases
GGCGTTTCGATGCAGGAGCGCCGGCCGACCGGCCGGTGCCGAAATCGGCGTGCCGTGCCGAGCGGCGCGCCGATTTTGCT
TTTGGCCGGGCGCGGGATTC

Product: Biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIQAQTVTPDATTAPEAVPVGMPSAPRWRVADVVKLYELPFNDLLFRAQQVHREHFDANAIQLSTLLSIKTGGCEEDCGY
CSQSSHHETELKASKLMDVDAVLDAARAAKANGATRFCMGAAWRSPKERHIEPLAEMIRGVKEMGLETCMTLGMLDDGQA
QALSEAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLITQLANMNPYPESV
PINNLVAIKGTPLENTAPLDPLEFVRTIAVARITMPKAMVRLSAGREQLDDAMQTLCFLAGANSMFYGDQLLTTSNPQAQ
KDRDLLARLGMRAEAAQQMPVDAAADAVKCAHAPGPVAN

Sequences:

>Translated_359_residues
MIQAQTVTPDATTAPEAVPVGMPSAPRWRVADVVKLYELPFNDLLFRAQQVHREHFDANAIQLSTLLSIKTGGCEEDCGY
CSQSSHHETELKASKLMDVDAVLDAARAAKANGATRFCMGAAWRSPKERHIEPLAEMIRGVKEMGLETCMTLGMLDDGQA
QALSEAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLITQLANMNPYPESV
PINNLVAIKGTPLENTAPLDPLEFVRTIAVARITMPKAMVRLSAGREQLDDAMQTLCFLAGANSMFYGDQLLTTSNPQAQ
KDRDLLARLGMRAEAAQQMPVDAAADAVKCAHAPGPVAN
>Mature_359_residues
MIQAQTVTPDATTAPEAVPVGMPSAPRWRVADVVKLYELPFNDLLFRAQQVHREHFDANAIQLSTLLSIKTGGCEEDCGY
CSQSSHHETELKASKLMDVDAVLDAARAAKANGATRFCMGAAWRSPKERHIEPLAEMIRGVKEMGLETCMTLGMLDDGQA
QALSEAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLITQLANMNPYPESV
PINNLVAIKGTPLENTAPLDPLEFVRTIAVARITMPKAMVRLSAGREQLDDAMQTLCFLAGANSMFYGDQLLTTSNPQAQ
KDRDLLARLGMRAEAAQQMPVDAAADAVKCAHAPGPVAN

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=310, Percent_Identity=62.5806451612903, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321725, Length=342, Percent_Identity=46.7836257309941, Blast_Score=323, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39038; Mature: 39038

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQAQTVTPDATTAPEAVPVGMPSAPRWRVADVVKLYELPFNDLLFRAQQVHREHFDANA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
IQLSTLLSIKTGGCEEDCGYCSQSSHHETELKASKLMDVDAVLDAARAAKANGATRFCMG
EEHHHHHEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AAWRSPKERHIEPLAEMIRGVKEMGLETCMTLGMLDDGQAQALSEAGLDYYNHNLDTSPE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHCCCCCCCHH
FYGQVISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLITQLANMNPYPESV
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCC
PINNLVAIKGTPLENTAPLDPLEFVRTIAVARITMPKAMVRLSAGREQLDDAMQTLCFLA
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GANSMFYGDQLLTTSNPQAQKDRDLLARLGMRAEAAQQMPVDAAADAVKCAHAPGPVAN
CCCCCEECCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIQAQTVTPDATTAPEAVPVGMPSAPRWRVADVVKLYELPFNDLLFRAQQVHREHFDANA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
IQLSTLLSIKTGGCEEDCGYCSQSSHHETELKASKLMDVDAVLDAARAAKANGATRFCMG
EEHHHHHEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AAWRSPKERHIEPLAEMIRGVKEMGLETCMTLGMLDDGQAQALSEAGLDYYNHNLDTSPE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHCCCCCCCHH
FYGQVISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLITQLANMNPYPESV
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCC
PINNLVAIKGTPLENTAPLDPLEFVRTIAVARITMPKAMVRLSAGREQLDDAMQTLCFLA
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GANSMFYGDQLLTTSNPQAQKDRDLLARLGMRAEAAQQMPVDAAADAVKCAHAPGPVAN
CCCCCEECCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA