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The map label for this gene is kefB [H]

Identifier: 238028651

GI number: 238028651

Start: 3543308

End: 3545314

Strand: Reverse

Name: kefB [H]

Synonym: bglu_1g31150

Alternate gene names: 238028651

Gene position: 3545314-3543308 (Counterclockwise)

Preceding gene: 238028663

Following gene: 238028650

Centisome position: 90.75

GC content: 69.26

Gene sequence:

>2007_bases
GTGATATCCCCGCTCGAAATGACGCTCCTGCTGCTGCTGGCTTCCGTGGCGGGCGTCGTCGTGTTCCGCTCGCTGAACCT
GCCGCCGATGCTCGGCTATCTGACCGTGGGCATCCTGGTCGGCCCGCACGCGCTCGGCTTCGCCGGCGATCTCGAGCGCG
CCGAGCACCTCGCCGAGTTCGGCGTGGTGTTCCTGATGTTCTCGATCGGCCTCGAATTCTCGCTGGCCAAGCTGCGGGCG
ATGCGGCGGCTGGTGTTCGGGCTCGGACTCGCGCAGGTGGTGGTCACGCTCGCGGTGGCGGTGCTGCTCGGCCTCGCGTT
CGAGCATTGGGTCCACATCACCTGGCAGGGCAGCGTCGCGCTGGGCGGCGCCCTGGCCATGTCGTCCACGGCGATCGTCT
CGAAGATGCTGGCCGAGCGGCTCGAGATCGAGACCGAGCATGGCCGCAACATCTTCGGCGTGCTGCTGTTCCAGGATCTG
GCCGTGGTGCCGCTCTTGATCGTGATCTCGGCGTTCGGCGGCGAATCGTCGAAGGATCTGGCCGTCACGCTCGGCCTCGC
GGCCGTGAAGATCGTGATCGCGCTGTCGCTGCTGCTGTTCGTCGGCCAGAAGTTCATGACACGCTGGTTCAACGTGGTGG
CGCGGCGCCGCTCGCAGGAGCTGTTCGTGCTGAACCTGCTGCTCGTCACGCTCGGCGCGGCATTCATTACCGACCGGTTC
GGGTTGTCGCTCGCGCTCGGCGCGTTCATCGCCGGCATGCTGATCTCCGAGACGCCTTACCGGCACCAGGTGGAGGAGGA
CATCAAGCCGTTCCGCGACGTGCTGCTCGGCCTGTTCTTCGTGACCACCGGGATGCTGCTGGACCCGAGCGTGATCTGGG
AGCACCCGTTCCTCGTGATCGGCTTCTTCGTGGGCCAGGTGCTGCTGAAGGCGGCCACCATCACCGGCCTGGCGCGGCTG
TTCGGTGCCACGCCCGGGGTGTCGATGCGCACCGGCCTGGGGCTCGCGCAGGCCGGCGAATTCGGCTTCGTGATGCTGAA
CCTGATCCTCGACCGCCATCTGGTGGACCCGACGCTGTTGCAGGCGATTCTCGCCTCGATGCTGCTGTCGATGCTGGCCG
CGCCGTTCCTGATTCAGAACGCCGACCGGATCGTGATGCGGCTGTCCTCGACGGAATGGATGCAGCAGTCGCTGCAGATG
ACGCGCATCGCGACCCAGAGCCTCAAGCAGCAGGGCCACGTGATCATCTGCGGCTACGGCCGGGCGGGCCAGAACCTGGC
GCGCATGCTCGAACAGGAGGGGCTCTCCTACGTGGCGCTCGATCTCGACCCGGACCGCGTCAGCGCGGCGGCCGCGGCCG
GCGAGTCGGTGGTGTTCGGCGACGCGGCGCGGCGCGAATCGCTGCTCGCGGCCGGCATCCACCGCGCCACGGCAGTGGCG
ATCACCTATGCGAACACGCCCTCGGCGTTGCGCGTGCTGCACAACGTCCATGAACTCGAGCCGACGCTGCCGGTGATCGT
GCGCACCGTGGACGACGCCGACCTCGACCACCTGCTCGCGGCCGGCGCGACCGAGGTGATCCCCGAGATCGTCGAGGGCA
GCCTGATGCTGGCCTCGCACACGCTGGTGGTGATGGGCGTGCCGATGCGGCGCGTGGTGCGGCGCGTGGAGGAGATGCGC
GACGCGCGCTACAGCCTGCTGCGCGGCTATTTCCACGGCGCCGATGACGCGGACGACGACGATCACGAGCAGGTGCGGCT
ACAATCGGTGCCCGTCGACGCGCGCTCGGATGCGGTCGGCCGCTCCCTCGAGGACGTCGGGCTCTACGCGCTCGGGCTCG
AGGTGACGGCGATCCGGCGCCACGGTATCCGCGGCGTCGAGCCGGACCCCGAGACGAAGCTGCGCGCGGCCGACATCGTC
GTGCTGCGCGGGCTGCCCGAGGCGCTCTCGCTGGCCGAGGAGCGGCTGTCGCGCCACCGCCGCGACGGCGGGCCGGCCCC
GGCCTGA

Upstream 100 bases:

>100_bases
TCCCGACGGAGTCCTGTCCTCGCCCACCGAGCCGCCGCTGCGCGCGGCCCCACCCGCGTCCGCCAGTCGCGACGCCCTGA
CGAACGAGGACCCTTAGCAC

Downstream 100 bases:

>100_bases
GCCGGGCGCGGCGGACGGGCACCGTATCATGTCGAGCGGCGCGGCCCCCGCAGCCGGATATTGTTCGGACCCCATCAATT
TCCGTCGGTCGCCACGGCCG

Product: Potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MISPLEMTLLLLLASVAGVVVFRSLNLPPMLGYLTVGILVGPHALGFAGDLERAEHLAEFGVVFLMFSIGLEFSLAKLRA
MRRLVFGLGLAQVVVTLAVAVLLGLAFEHWVHITWQGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDL
AVVPLLIVISAFGGESSKDLAVTLGLAAVKIVIALSLLLFVGQKFMTRWFNVVARRRSQELFVLNLLLVTLGAAFITDRF
GLSLALGAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWEHPFLVIGFFVGQVLLKAATITGLARL
FGATPGVSMRTGLGLAQAGEFGFVMLNLILDRHLVDPTLLQAILASMLLSMLAAPFLIQNADRIVMRLSSTEWMQQSLQM
TRIATQSLKQQGHVIICGYGRAGQNLARMLEQEGLSYVALDLDPDRVSAAAAAGESVVFGDAARRESLLAAGIHRATAVA
ITYANTPSALRVLHNVHELEPTLPVIVRTVDDADLDHLLAAGATEVIPEIVEGSLMLASHTLVVMGVPMRRVVRRVEEMR
DARYSLLRGYFHGADDADDDDHEQVRLQSVPVDARSDAVGRSLEDVGLYALGLEVTAIRRHGIRGVEPDPETKLRAADIV
VLRGLPEALSLAEERLSRHRRDGGPAPA

Sequences:

>Translated_668_residues
MISPLEMTLLLLLASVAGVVVFRSLNLPPMLGYLTVGILVGPHALGFAGDLERAEHLAEFGVVFLMFSIGLEFSLAKLRA
MRRLVFGLGLAQVVVTLAVAVLLGLAFEHWVHITWQGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDL
AVVPLLIVISAFGGESSKDLAVTLGLAAVKIVIALSLLLFVGQKFMTRWFNVVARRRSQELFVLNLLLVTLGAAFITDRF
GLSLALGAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWEHPFLVIGFFVGQVLLKAATITGLARL
FGATPGVSMRTGLGLAQAGEFGFVMLNLILDRHLVDPTLLQAILASMLLSMLAAPFLIQNADRIVMRLSSTEWMQQSLQM
TRIATQSLKQQGHVIICGYGRAGQNLARMLEQEGLSYVALDLDPDRVSAAAAAGESVVFGDAARRESLLAAGIHRATAVA
ITYANTPSALRVLHNVHELEPTLPVIVRTVDDADLDHLLAAGATEVIPEIVEGSLMLASHTLVVMGVPMRRVVRRVEEMR
DARYSLLRGYFHGADDADDDDHEQVRLQSVPVDARSDAVGRSLEDVGLYALGLEVTAIRRHGIRGVEPDPETKLRAADIV
VLRGLPEALSLAEERLSRHRRDGGPAPA
>Mature_668_residues
MISPLEMTLLLLLASVAGVVVFRSLNLPPMLGYLTVGILVGPHALGFAGDLERAEHLAEFGVVFLMFSIGLEFSLAKLRA
MRRLVFGLGLAQVVVTLAVAVLLGLAFEHWVHITWQGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDL
AVVPLLIVISAFGGESSKDLAVTLGLAAVKIVIALSLLLFVGQKFMTRWFNVVARRRSQELFVLNLLLVTLGAAFITDRF
GLSLALGAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWEHPFLVIGFFVGQVLLKAATITGLARL
FGATPGVSMRTGLGLAQAGEFGFVMLNLILDRHLVDPTLLQAILASMLLSMLAAPFLIQNADRIVMRLSSTEWMQQSLQM
TRIATQSLKQQGHVIICGYGRAGQNLARMLEQEGLSYVALDLDPDRVSAAAAAGESVVFGDAARRESLLAAGIHRATAVA
ITYANTPSALRVLHNVHELEPTLPVIVRTVDDADLDHLLAAGATEVIPEIVEGSLMLASHTLVVMGVPMRRVVRRVEEMR
DARYSLLRGYFHGADDADDDDHEQVRLQSVPVDARSDAVGRSLEDVGLYALGLEVTAIRRHGIRGVEPDPETKLRAADIV
VLRGLPEALSLAEERLSRHRRDGGPAPA

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=347, Percent_Identity=28.5302593659942, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1786232, Length=634, Percent_Identity=30.7570977917981, Blast_Score=252, Evalue=5e-68,
Organism=Escherichia coli, GI1789749, Length=562, Percent_Identity=33.0960854092527, Blast_Score=252, Evalue=6e-68,
Organism=Escherichia coli, GI1786685, Length=534, Percent_Identity=29.4007490636704, Blast_Score=205, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 72324; Mature: 72324

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPLEMTLLLLLASVAGVVVFRSLNLPPMLGYLTVGILVGPHALGFAGDLERAEHLAEF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVVFLMFSIGLEFSLAKLRAMRRLVFGLGLAQVVVTLAVAVLLGLAFEHWVHITWQGSVA
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCEE
LGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLIVISAFGGESSKDL
ECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AVTLGLAAVKIVIALSLLLFVGQKFMTRWFNVVARRRSQELFVLNLLLVTLGAAFITDRF
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GLSLALGAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWEHPFLVI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHCCHHHHH
GFFVGQVLLKAATITGLARLFGATPGVSMRTGLGLAQAGEFGFVMLNLILDRHLVDPTLL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHCCCCHHHHHHHHHHHHHCCCHHHH
QAILASMLLSMLAAPFLIQNADRIVMRLSSTEWMQQSLQMTRIATQSLKQQGHVIICGYG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
RAGQNLARMLEQEGLSYVALDLDPDRVSAAAAAGESVVFGDAARRESLLAAGIHRATAVA
CCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHEEE
ITYANTPSALRVLHNVHELEPTLPVIVRTVDDADLDHLLAAGATEVIPEIVEGSLMLASH
EEECCCHHHHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHCCHHHHHHHHHCCCCEEEEC
TLVVMGVPMRRVVRRVEEMRDARYSLLRGYFHGADDADDDDHEQVRLQSVPVDARSDAVG
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEHHCCCCCCCCHHHC
RSLEDVGLYALGLEVTAIRRHGIRGVEPDPETKLRAADIVVLRGLPEALSLAEERLSRHR
CCHHHCCCHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHEEEECCCCHHHHHHHHHHHHHH
RDGGPAPA
CCCCCCCC
>Mature Secondary Structure
MISPLEMTLLLLLASVAGVVVFRSLNLPPMLGYLTVGILVGPHALGFAGDLERAEHLAEF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVVFLMFSIGLEFSLAKLRAMRRLVFGLGLAQVVVTLAVAVLLGLAFEHWVHITWQGSVA
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCEE
LGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLIVISAFGGESSKDL
ECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AVTLGLAAVKIVIALSLLLFVGQKFMTRWFNVVARRRSQELFVLNLLLVTLGAAFITDRF
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GLSLALGAFIAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTTGMLLDPSVIWEHPFLVI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHCCHHHHH
GFFVGQVLLKAATITGLARLFGATPGVSMRTGLGLAQAGEFGFVMLNLILDRHLVDPTLL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHCCCCHHHHHHHHHHHHHCCCHHHH
QAILASMLLSMLAAPFLIQNADRIVMRLSSTEWMQQSLQMTRIATQSLKQQGHVIICGYG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
RAGQNLARMLEQEGLSYVALDLDPDRVSAAAAAGESVVFGDAARRESLLAAGIHRATAVA
CCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHEEE
ITYANTPSALRVLHNVHELEPTLPVIVRTVDDADLDHLLAAGATEVIPEIVEGSLMLASH
EEECCCHHHHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHCCHHHHHHHHHCCCCEEEEC
TLVVMGVPMRRVVRRVEEMRDARYSLLRGYFHGADDADDDDHEQVRLQSVPVDARSDAVG
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEHHCCCCCCCCHHHC
RSLEDVGLYALGLEVTAIRRHGIRGVEPDPETKLRAADIVVLRGLPEALSLAEERLSRHR
CCHHHCCCHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHEEEECCCCHHHHHHHHHHHHHH
RDGGPAPA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA