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The map label for this gene is ubiE [H]

Identifier: 238028619

GI number: 238028619

Start: 3504290

End: 3505021

Strand: Reverse

Name: ubiE [H]

Synonym: bglu_1g30830

Alternate gene names: 238028619

Gene position: 3505021-3504290 (Counterclockwise)

Preceding gene: 238028620

Following gene: 238028618

Centisome position: 89.72

GC content: 60.93

Gene sequence:

>732_bases
ATGAGCAAAACCCACTTCGGCTTCGAGACCGTCGACGAGCAAGACAAGGCGAAAAAGGTGGCGGGAGTGTTTCACTCGGT
CGCGAGCAACTACGACCTGATGAACGACTTGATGTCGGCCGGCATGCATCGCGCCTGGAAGGCCTTCACGATCGCGCAGG
CGAACGTGCGGCCCGGCTACAAGGTGCTCGACATCGCGGCCGGCACCGGCGACCTGACCCGCGCGTTCGCGAAGGCCGCC
GGCCCGACGGGCGAGGTCTGGCATACCGACATCAACGAATCGATGCTGCGCGTGGGGCGCGACCGGCTGCTCGACAAGGG
CGTGGTCACGCCGTCGCTGCTCTGCGATGCCGAGAAGATCCCGTTTCCCGACAACCATTTCGACGTGGTGACGGTGGCGT
TCGGGCTGCGCAACATGACCCACAAGGAGGCCGCTCTGGCCGAGATGCGGCGCGTGACGCGGCCCGGCGGACGCGTGATG
GTGCTCGAATTCTCGAAAGTGTGGGAGCCGCTGAAAAAAGCCTACGATCTGTATTCTTTCAAAGTATTACCGTGGCTCGG
CGACAAGTTCGCGAAAGATGCCGGAAGTTACCAGTATCTTGCCGAGTCGATCCGGATGCATCCGGATCAGGAGACGCTGA
AGACGATGATGGAGCAGGCGGGCCTCGATGCCGTCAAATATTACAATTTGTCAGGTGGCGTGGTAGCGTTACACCACGGA
ACCAAGTACTAA

Upstream 100 bases:

>100_bases
TGCCCGCCGCCGGCGGCCAGAGCGGTCACTGTCACTGACTACCGATTGATCCGACGGCGCCCTCCTGGCGCCGTCTTGCC
ATTTCCAGAGGATTCACGCG

Downstream 100 bases:

>100_bases
GGGGTTCTGTCCATACAGTTACCTTTCATTTTGGAGATGGAGATGTCCGAGTCCCGTGCGTTGAACAATGGCAGCAAGCA
GCGGAGGCCGTGGGCACGAC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSKTHFGFETVDEQDKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGYKVLDIAAGTGDLTRAFAKAA
GPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKIPFPDNHFDVVTVAFGLRNMTHKEAALAEMRRVTRPGGRVM
VLEFSKVWEPLKKAYDLYSFKVLPWLGDKFAKDAGSYQYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHHG
TKY

Sequences:

>Translated_243_residues
MSKTHFGFETVDEQDKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGYKVLDIAAGTGDLTRAFAKAA
GPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKIPFPDNHFDVVTVAFGLRNMTHKEAALAEMRRVTRPGGRVM
VLEFSKVWEPLKKAYDLYSFKVLPWLGDKFAKDAGSYQYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHHG
TKY
>Mature_242_residues
SKTHFGFETVDEQDKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGYKVLDIAAGTGDLTRAFAKAAG
PTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKIPFPDNHFDVVTVAFGLRNMTHKEAALAEMRRVTRPGGRVMV
LEFSKVWEPLKKAYDLYSFKVLPWLGDKFAKDAGSYQYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHHGT
KY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=277, Percent_Identity=43.3212996389892, Blast_Score=221, Evalue=4e-58,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=60.4938271604938, Blast_Score=300, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI17557039, Length=244, Percent_Identity=45.0819672131148, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6323526, Length=257, Percent_Identity=41.2451361867704, Blast_Score=166, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24641702, Length=255, Percent_Identity=46.2745098039216, Blast_Score=220, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27089; Mature: 26958

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTHFGFETVDEQDKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KVLDIAAGTGDLTRAFAKAAGPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKI
EEEEEECCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPDNHFDVVTVAFGLRNMTHKEAALAEMRRVTRPGGRVMVLEFSKVWEPLKKAYDLYSF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDKFAKDAGSYQYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHHG
EECHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEECCC
TKY
CCC
>Mature Secondary Structure 
SKTHFGFETVDEQDKAKKVAGVFHSVASNYDLMNDLMSAGMHRAWKAFTIAQANVRPGY
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KVLDIAAGTGDLTRAFAKAAGPTGEVWHTDINESMLRVGRDRLLDKGVVTPSLLCDAEKI
EEEEEECCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPDNHFDVVTVAFGLRNMTHKEAALAEMRRVTRPGGRVMVLEFSKVWEPLKKAYDLYSF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDKFAKDAGSYQYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSGGVVALHHG
EECHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEECCC
TKY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA