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The map label for this gene is glpF [H]

Identifier: 238028579

GI number: 238028579

Start: 3457965

End: 3458681

Strand: Direct

Name: glpF [H]

Synonym: bglu_1g30430

Alternate gene names: 238028579

Gene position: 3457965-3458681 (Clockwise)

Preceding gene: 238028578

Following gene: 238028580

Centisome position: 88.52

GC content: 68.62

Gene sequence:

>717_bases
ATGTCACCTTATATTGCGGAGTTCATCGGCACGGCAATCCTCGTGCTGCTCGGCAACGGCGCGGTCGCGAACGTGCTCCT
GGCGAAAACGAAAGGTCGCGGCGCCGACCTGATCGTCATCGTGATGGGCTGGGCGATGGCCGTGTTCGTGGCCGTCTACG
TGACCGCGAAGTTCAGCGGCGCACACCTGAATCCGATCGTCACGATCAGTCTCGCGCTGGCCGGCAAGTTCGCGTGGTCG
AAGGTCGGCGGCTACATCGCCGCGCAGATGCTGGGGGGGATGGCAGGCGCGCTGCTGGTATGGCTCGCCTATCGCCAGCA
TTTCGCCAAGGAGGCCGACCCCGACCTGAAGCTGGCCGTGTTCTGCACCGCGCCCGCAATCCGCAGCGTCGTGCACAACG
TGCTGACCGAGGCGATCTGCACCTTCGTGCTGATCCTCGGCGTGCTGTATCTGGCGGCCCCGCAGGTCGGGCTGGGCGCG
CTCGACGCGCTGCCGGTCGGGCTGCTGGTGCTCGGCATCGGCATCTCGCTCGGCGGCCCGACCGGCTATGCGATGAGCCC
GGCGCGCGATCTGTCGCCGCGCATCATGCATGCGCTGCTGCCGATCCCCGGCAAGCGCGACAGCGACTGGCGCTACGCCT
GGATTCCGGTGCTCGGCCCGCTCGCGGGCGGCGCGGCGGCGGCCTGCCTCTACCTGGCCCTGCACCGGACGCACTGA

Upstream 100 bases:

>100_bases
CGACCGCGGCGGCCGGCAGCGGGCAACACCACCACGATTCATAACGAGCAGGCGGACCGTGCAGCGCGCGAGTCCGCCGC
ATTGGTAGAGAGACACCCAA

Downstream 100 bases:

>100_bases
GCGCAGCTTGCCGCAACAGCACGGGGCACGCGGGTCGTCCGGCTCGCGTGCGCCGTCTTTCATGGTCCCTCCCAACGTGC
CACGCATCGGTGCCTGCGCG

Product: putative glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSPYIAEFIGTAILVLLGNGAVANVLLAKTKGRGADLIVIVMGWAMAVFVAVYVTAKFSGAHLNPIVTISLALAGKFAWS
KVGGYIAAQMLGGMAGALLVWLAYRQHFAKEADPDLKLAVFCTAPAIRSVVHNVLTEAICTFVLILGVLYLAAPQVGLGA
LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWIPVLGPLAGGAAAACLYLALHRTH

Sequences:

>Translated_238_residues
MSPYIAEFIGTAILVLLGNGAVANVLLAKTKGRGADLIVIVMGWAMAVFVAVYVTAKFSGAHLNPIVTISLALAGKFAWS
KVGGYIAAQMLGGMAGALLVWLAYRQHFAKEADPDLKLAVFCTAPAIRSVVHNVLTEAICTFVLILGVLYLAAPQVGLGA
LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWIPVLGPLAGGAAAACLYLALHRTH
>Mature_237_residues
SPYIAEFIGTAILVLLGNGAVANVLLAKTKGRGADLIVIVMGWAMAVFVAVYVTAKFSGAHLNPIVTISLALAGKFAWSK
VGGYIAAQMLGGMAGALLVWLAYRQHFAKEADPDLKLAVFCTAPAIRSVVHNVLTEAICTFVLILGVLYLAAPQVGLGAL
DALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWIPVLGPLAGGAAAACLYLALHRTH

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=34.2741935483871, Blast_Score=124, Evalue=5e-29,
Organism=Homo sapiens, GI4826645, Length=238, Percent_Identity=33.6134453781513, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI22538420, Length=249, Percent_Identity=31.7269076305221, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4502187, Length=243, Percent_Identity=31.6872427983539, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=34.5238095238095, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71994009, Length=243, Percent_Identity=31.2757201646091, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17544068, Length=241, Percent_Identity=30.7053941908714, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17531429, Length=243, Percent_Identity=26.7489711934156, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531431, Length=243, Percent_Identity=26.7489711934156, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17558372, Length=231, Percent_Identity=29.4372294372294, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71992966, Length=239, Percent_Identity=26.7782426778243, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993722, Length=230, Percent_Identity=30, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=211, Percent_Identity=32.7014218009479, Blast_Score=95, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322985, Length=279, Percent_Identity=26.8817204301075, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24762342, Length=236, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24808; Mature: 24677

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPYIAEFIGTAILVLLGNGAVANVLLAKTKGRGADLIVIVMGWAMAVFVAVYVTAKFSG
CCCHHHHHHHHHHHHHHCCCHHHHHHEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCC
AHLNPIVTISLALAGKFAWSKVGGYIAAQMLGGMAGALLVWLAYRQHFAKEADPDLKLAV
CCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
FCTAPAIRSVVHNVLTEAICTFVLILGVLYLAAPQVGLGALDALPVGLLVLGIGISLGGP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWIPVLGPLAGGAAAACLYLALHRTH
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPYIAEFIGTAILVLLGNGAVANVLLAKTKGRGADLIVIVMGWAMAVFVAVYVTAKFSG
CCHHHHHHHHHHHHHHCCCHHHHHHEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCC
AHLNPIVTISLALAGKFAWSKVGGYIAAQMLGGMAGALLVWLAYRQHFAKEADPDLKLAV
CCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
FCTAPAIRSVVHNVLTEAICTFVLILGVLYLAAPQVGLGALDALPVGLLVLGIGISLGGP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWIPVLGPLAGGAAAACLYLALHRTH
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]