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The map label for this gene is rotA [H]

Identifier: 238027922

GI number: 238027922

Start: 2598032

End: 2598610

Strand: Direct

Name: rotA [H]

Synonym: bglu_1g23640

Alternate gene names: 238027922

Gene position: 2598032-2598610 (Clockwise)

Preceding gene: 238027921

Following gene: 238027923

Centisome position: 66.51

GC content: 64.25

Gene sequence:

>579_bases
ATGAAACGTCTGTTGTTGGCGCTCGGCAGCGCCGCCTTCGTCGCGACCGCACCCGTTTACGCCCAAACGGCGGCCGCCGC
GCATCCGGTCGTGCAGTTGAAGACCACGCAGGGCGACATCCGCGTCGAGCTGTATCCCGAGAAGGCGCCGAAGACGGTGG
CGAACTTCCTCGACTACGTGAAGGCCGGCCAATACAACGGCACGATCTTCCATCGCGTGATTCCCGGCTTCATGATCCAG
GGCGGCGGCTACAAGGAGAATTTCGACGAGAAGCCCACCCGCGCGCCGATCCCGCTCGAAAGCCGCAACGGCCTGAAGAA
CCTGACGGGCACCATCGCGATGGCGCGCACCAGCGATCCGAACTCGGCCACCGCGCAGTTCTTCATCAACACCGTCGATA
ACGCCGGCCTCGACTACCCGAACCCCGACGGCAACGGCTATGCGGTGTTCGGCAAGGTCGTCTCGGGCATGGACGTCGTC
AAGAAGATCGAGGCGGCGCCCACCACGGTGCACGGCTCGATGCGCGACGTCCCCCAGAAGCCGGTCGTGATCGAATCGGC
CAGCATCGTCAGCAAGTAA

Upstream 100 bases:

>100_bases
GCTCGCTCGCGGTGCCGCCCTACCTGGCGCCATCGAACTGACGCGGGAATCGGACGCGCGGCAGGGCTTCCCAATCCATT
CACTCAACCAGAGGATCGTC

Downstream 100 bases:

>100_bases
ACCCCGGGCGCGCTGCGCCCTGCCACGCGGCCGCGTCGTCCCGACCGGCCGCGTGCCGTTTCACCCACCTCTAAAGAAGG
AACCATCATGGTTGAACTGC

Product: peptidyl-prolyl cis-trans isomerase A

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKRLLLALGSAAFVATAPVYAQTAAAAHPVVQLKTTQGDIRVELYPEKAPKTVANFLDYVKAGQYNGTIFHRVIPGFMIQ
GGGYKENFDEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNAGLDYPNPDGNGYAVFGKVVSGMDVV
KKIEAAPTTVHGSMRDVPQKPVVIESASIVSK

Sequences:

>Translated_192_residues
MKRLLLALGSAAFVATAPVYAQTAAAAHPVVQLKTTQGDIRVELYPEKAPKTVANFLDYVKAGQYNGTIFHRVIPGFMIQ
GGGYKENFDEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNAGLDYPNPDGNGYAVFGKVVSGMDVV
KKIEAAPTTVHGSMRDVPQKPVVIESASIVSK
>Mature_192_residues
MKRLLLALGSAAFVATAPVYAQTAAAAHPVVQLKTTQGDIRVELYPEKAPKTVANFLDYVKAGQYNGTIFHRVIPGFMIQ
GGGYKENFDEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNAGLDYPNPDGNGYAVFGKVVSGMDVV
KKIEAAPTTVHGSMRDVPQKPVVIESASIVSK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=170, Percent_Identity=32.9411764705882, Blast_Score=84, Evalue=6e-17,
Organism=Homo sapiens, GI22547212, Length=200, Percent_Identity=32.5, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI7657473, Length=200, Percent_Identity=32.5, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI22547215, Length=200, Percent_Identity=32.5, Blast_Score=80, Evalue=9e-16,
Organism=Homo sapiens, GI24308049, Length=164, Percent_Identity=35.3658536585366, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI64276486, Length=171, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI7706339, Length=152, Percent_Identity=33.5526315789474, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI10863927, Length=133, Percent_Identity=36.0902255639098, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI310110104, Length=138, Percent_Identity=35.5072463768116, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI5454154, Length=162, Percent_Identity=33.9506172839506, Blast_Score=70, Evalue=9e-13,
Organism=Homo sapiens, GI5031987, Length=182, Percent_Identity=33.5164835164835, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310129267, Length=133, Percent_Identity=34.5864661654135, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI310120620, Length=133, Percent_Identity=34.5864661654135, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4758950, Length=158, Percent_Identity=35.4430379746835, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789763, Length=190, Percent_Identity=54.2105263157895, Blast_Score=187, Evalue=4e-49,
Organism=Escherichia coli, GI1786736, Length=162, Percent_Identity=53.7037037037037, Blast_Score=176, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI71980594, Length=137, Percent_Identity=39.4160583941606, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71980590, Length=137, Percent_Identity=39.4160583941606, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17539496, Length=154, Percent_Identity=34.4155844155844, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17565860, Length=216, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17566266, Length=174, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17559074, Length=174, Percent_Identity=33.9080459770115, Blast_Score=73, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17552782, Length=174, Percent_Identity=33.9080459770115, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17506311, Length=171, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532641, Length=202, Percent_Identity=31.1881188118812, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17506307, Length=187, Percent_Identity=30.4812834224599, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323562, Length=173, Percent_Identity=35.2601156069364, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320510, Length=205, Percent_Identity=32.6829268292683, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320359, Length=153, Percent_Identity=33.9869281045752, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19922912, Length=149, Percent_Identity=37.5838926174497, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17986117, Length=170, Percent_Identity=32.3529411764706, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45549139, Length=167, Percent_Identity=35.3293413173653, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922376, Length=170, Percent_Identity=31.7647058823529, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24664125, Length=189, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21357783, Length=189, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17647301, Length=184, Percent_Identity=33.1521739130435, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130249, Length=160, Percent_Identity=35.625, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24652460, Length=148, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136360, Length=200, Percent_Identity=30, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20509; Mature: 20509

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLLALGSAAFVATAPVYAQTAAAAHPVVQLKTTQGDIRVELYPEKAPKTVANFLDYV
CCCEEEHHCCCHHHHCCCHHHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHH
KAGQYNGTIFHRVIPGFMIQGGGYKENFDEKPTRAPIPLESRNGLKNLTGTIAMARTSDP
HCCCCCCEEHEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCCC
NSATAQFFINTVDNAGLDYPNPDGNGYAVFGKVVSGMDVVKKIEAAPTTVHGSMRDVPQK
CCCEEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEECCCHHCCCCC
PVVIESASIVSK
CEEEECCHHHCC
>Mature Secondary Structure
MKRLLLALGSAAFVATAPVYAQTAAAAHPVVQLKTTQGDIRVELYPEKAPKTVANFLDYV
CCCEEEHHCCCHHHHCCCHHHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHH
KAGQYNGTIFHRVIPGFMIQGGGYKENFDEKPTRAPIPLESRNGLKNLTGTIAMARTSDP
HCCCCCCEEHEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCCC
NSATAQFFINTVDNAGLDYPNPDGNGYAVFGKVVSGMDVVKKIEAAPTTVHGSMRDVPQK
CCCEEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEECCCHHCCCCC
PVVIESASIVSK
CEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]