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The map label for this gene is queA [H]

Identifier: 238026275

GI number: 238026275

Start: 639236

End: 640288

Strand: Reverse

Name: queA [H]

Synonym: bglu_1g06080

Alternate gene names: 238026275

Gene position: 640288-639236 (Counterclockwise)

Preceding gene: 238026280

Following gene: 238026274

Centisome position: 16.39

GC content: 71.89

Gene sequence:

>1053_bases
ATGTTCAAGCTTTCCGATTTCGATTTCGACCTGCCGCCCGAGTTGATCGCGCAGACGGCGCTGCCCGAGCGCAGCGCGAG
CCGGCTCCTCGAGGTGGGCGGCACCGCCGGGCCCGCGCGGCTCGTGGATCGCCGCTTCACCGAGCTGCCCGACTGCCTCG
CGGCCGGCGACCTGCTGGTCTTCAACGACACCAAGGTGCTCAAGGCGCGCTTCTTCGGCCAGAAGGCGAGCGGCGGCCGG
ATCGAGGTGCTGATCGAGCGCGTGACCGGCCCGCGCACGGCGCTGGCGCAGATCCGCGCCAGCAAGAGCCCGGCGGCGGG
CGCCACGCTGACGCTGGCCGACGCGTTCGAGGTGACGGTCGGCGAGCGCGTCGAGCCGTTCTTCACGCTGCATTTCCCCG
ACGATTGCCTGACGCTGATCGAGCGGCACGGCCGCCTGCCGCTGCCGCCCTACATCGAGCACGATCCCGACGCGACCGAC
GAGACGCGCTACCAGACCGTATTCGCCAGCAATCCGGGCGCGGTGGCCGCACCCACCGCCGGCCTGCACTTCGACGAGGC
GCTGCTCGCCACGCTGCGCGCGCGCGGCGTCGAGACCGCCACGCTCACGCTGCACGTGGGCGCCGGCACCTTCCAGCCGG
TGCGCGTCGACAACATCGCCGACCACCGGATGCACAGCGAGTGGTACGAGCTGACCGACGCGCTGGTTGAGCGGATCGCG
GCCACCCGCGCGCGCGGCGGCCGCGTGATCGCGGTCGGCACCACCTCGATGCGCGCGCTGGAAGCGGCCGCGCGCGACGC
CGAGGCGGCCGGCCGGCCGCTCGCGGCCACCCGCGCCGAGACCGACATCTTCATCACGCCGGGCTACCGGTTCCGCGTGG
TGGACCGGCTCGTCACCAATTTCCATCTGCCGAAATCGACGCTGCTGATGCTGGTCTCGGCGTTCGCCGGCATCGAGCCG
ATCCGCGCCGCCTACCGCCACGCGATCGCCGAACGCTACCGTTTCTTCAGCTACGGCGACGCGATGCTGCTCACGCGGCG
CGAGCCCGACTGA

Upstream 100 bases:

>100_bases
CGCGCGACCCCGCCATCATAGCGGCTCGCGCCGGCACTGCGCGGCATGCACGCCGCCGCGCCCCGGCGACGCACCGCGTC
TGGTCCCCATTTCCCACTTC

Downstream 100 bases:

>100_bases
CGCGGCCGTCCCCGGCCCTGCCCTTTTCCCGCCGCCGGCACGTTCACGCGCCGGCGGCATTCACCACCACTCGCAACGCG
CGCCGGACTGTCTTCCGGTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MFKLSDFDFDLPPELIAQTALPERSASRLLEVGGTAGPARLVDRRFTELPDCLAAGDLLVFNDTKVLKARFFGQKASGGR
IEVLIERVTGPRTALAQIRASKSPAAGATLTLADAFEVTVGERVEPFFTLHFPDDCLTLIERHGRLPLPPYIEHDPDATD
ETRYQTVFASNPGAVAAPTAGLHFDEALLATLRARGVETATLTLHVGAGTFQPVRVDNIADHRMHSEWYELTDALVERIA
ATRARGGRVIAVGTTSMRALEAAARDAEAAGRPLAATRAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGIEP
IRAAYRHAIAERYRFFSYGDAMLLTRREPD

Sequences:

>Translated_350_residues
MFKLSDFDFDLPPELIAQTALPERSASRLLEVGGTAGPARLVDRRFTELPDCLAAGDLLVFNDTKVLKARFFGQKASGGR
IEVLIERVTGPRTALAQIRASKSPAAGATLTLADAFEVTVGERVEPFFTLHFPDDCLTLIERHGRLPLPPYIEHDPDATD
ETRYQTVFASNPGAVAAPTAGLHFDEALLATLRARGVETATLTLHVGAGTFQPVRVDNIADHRMHSEWYELTDALVERIA
ATRARGGRVIAVGTTSMRALEAAARDAEAAGRPLAATRAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGIEP
IRAAYRHAIAERYRFFSYGDAMLLTRREPD
>Mature_350_residues
MFKLSDFDFDLPPELIAQTALPERSASRLLEVGGTAGPARLVDRRFTELPDCLAAGDLLVFNDTKVLKARFFGQKASGGR
IEVLIERVTGPRTALAQIRASKSPAAGATLTLADAFEVTVGERVEPFFTLHFPDDCLTLIERHGRLPLPPYIEHDPDATD
ETRYQTVFASNPGAVAAPTAGLHFDEALLATLRARGVETATLTLHVGAGTFQPVRVDNIADHRMHSEWYELTDALVERIA
ATRARGGRVIAVGTTSMRALEAAARDAEAAGRPLAATRAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGIEP
IRAAYRHAIAERYRFFSYGDAMLLTRREPD

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=348, Percent_Identity=54.0229885057471, Blast_Score=353, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38341; Mature: 38341

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLSDFDFDLPPELIAQTALPERSASRLLEVGGTAGPARLVDRRFTELPDCLAAGDLLV
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHCCCEEE
FNDTKVLKARFFGQKASGGRIEVLIERVTGPRTALAQIRASKSPAAGATLTLADAFEVTV
ECCCHHHHHHHCCCCCCCCEEEEEEEHHCCCHHHHHHHHCCCCCCCCCEEEEECCEEECC
GERVEPFFTLHFPDDCLTLIERHGRLPLPPYIEHDPDATDETRYQTVFASNPGAVAAPTA
CCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCC
GLHFDEALLATLRARGVETATLTLHVGAGTFQPVRVDNIADHRMHSEWYELTDALVERIA
CCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
ATRARGGRVIAVGTTSMRALEAAARDAEAAGRPLAATRAETDIFITPGYRFRVVDRLVTN
HHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCEEEECCCCHHHHHHHHHHH
FHLPKSTLLMLVSAFAGIEPIRAAYRHAIAERYRFFSYGDAMLLTRREPD
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
>Mature Secondary Structure
MFKLSDFDFDLPPELIAQTALPERSASRLLEVGGTAGPARLVDRRFTELPDCLAAGDLLV
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHCCCEEE
FNDTKVLKARFFGQKASGGRIEVLIERVTGPRTALAQIRASKSPAAGATLTLADAFEVTV
ECCCHHHHHHHCCCCCCCCEEEEEEEHHCCCHHHHHHHHCCCCCCCCCEEEEECCEEECC
GERVEPFFTLHFPDDCLTLIERHGRLPLPPYIEHDPDATDETRYQTVFASNPGAVAAPTA
CCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCC
GLHFDEALLATLRARGVETATLTLHVGAGTFQPVRVDNIADHRMHSEWYELTDALVERIA
CCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
ATRARGGRVIAVGTTSMRALEAAARDAEAAGRPLAATRAETDIFITPGYRFRVVDRLVTN
HHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCEEEECCCCHHHHHHHHHHH
FHLPKSTLLMLVSAFAGIEPIRAAYRHAIAERYRFFSYGDAMLLTRREPD
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA