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The map label for this gene is tgt [H]

Identifier: 238026274

GI number: 238026274

Start: 637900

End: 639120

Strand: Reverse

Name: tgt [H]

Synonym: bglu_1g06070

Alternate gene names: 238026274

Gene position: 639120-637900 (Counterclockwise)

Preceding gene: 238026275

Following gene: 238026273

Centisome position: 16.36

GC content: 68.88

Gene sequence:

>1221_bases
ATGACCGATCGCCGACCGGAACCCGTGCCGACCCACGTGCCGCCCGAGAACGGGCTGAACTTCGAACTGCTGACGACCGA
CGGCCACGCGCGCCGGGGCCGCGTCACGCTCAACCACGGCGTGGTCGAGACGCCGATCTTCATGCCGGTCGGCACCTACG
GCACCGTCAAGGCGGTGCAGCCGCGCGAGCTGCGCGAGATGCAGGCGCAGATCATTCTCGGCAACACCTTCCACCTGTGG
CTGCGCCCGGGCCTCGAGACGATCGGCGCGCACGGCGGCCTGCACCGTTTCATGGGCTGGGACAGGCCGATCCTGACCGA
CTCGGGCGGCTTCCAGGTGTTCTCGCTCGGCGAGCTGCGCAAGATCACCGAGGATGGCGTGACGTTCGCCTCGCCGATCA
ACGGCGACAAGCTGTTCCTGTCGCCCGAGGTGTCGATGCAGATCCAGAAGGTGCTGAACTCCGATATCGTCATGCAGTTC
GACGAATGCACGCCCTACGCGACCGGCGGCGTGCCGACCACCCACCGGGAGGCGGCCGAGTCGATGCGCATGTCGCTGCG
CTGGGCGAAGCGCTCGCTCGACGAATTCGACCGGCTCGGCAACCCCAACGCGCTGTTCGGCATCGTGCAGGGCGGCATGT
TCGAGGACCTGCGCGACGAGTCGCTGGCCGGCCTTGCCGCGCTCGACTTCCACGGCTACGCGATCGGCGGGCTGTCGGTG
GGCGAGCCGAAGGAGGACATGATGCGCGTGCTGCGCCATGTCGGCCCGAAGCTGCCGGCGCACAAGCCGCACTACCTGAT
GGGGGTGGGCACCCCCGAGGATCTGGTCGAGGGCGTGGCCAACGGCGTCGACATGTTCGACTGCGTGATGCCGACCCGCA
ACGCGCGCAACGGCTGGCTGTTCACGCGCTTCGGCGACGTGAAGATCCGCAACGCCACGCACCGCAACTCGCTCAAGCCG
CTCGACGAGCGCTGCGGCTGCTACACCTGCCGCCACTTCACGCGCGGCTACCTGCATCACCTGCACCGCGTGGGCGAGAT
CCTCGGCGCGCAGCTGAACACGATCCACAACCTGCACTACTACCTGGAGCTGATGAGCGAGATCCGCGCGGCGATCGAAA
CCCACACGTTCGACGCGTTCCGCGCACGCTTCGCGGCCGACCGCGCACGCGGCGTGGAGGCCGAGCCGGTCGCGCCGGGC
GAGGCGGCCGCGGGCGGATGA

Upstream 100 bases:

>100_bases
CCCTGCCCTTTTCCCGCCGCCGGCACGTTCACGCGCCGGCGGCATTCACCACCACTCGCAACGCGCGCCGGACTGTCTTC
CGGTGGCCAGGAGTACCTGA

Downstream 100 bases:

>100_bases
CGGCCGCGCATGTCACGGCGGCACGCAATCGCGCCGCCTCCCGGGATGCCTCATACGCCTGTCGAATGCCGCGGCGATGC
TCGCGAGGCCGCGTTTTTCA

Product: Queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTDRRPEPVPTHVPPENGLNFELLTTDGHARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELREMQAQIILGNTFHLW
LRPGLETIGAHGGLHRFMGWDRPILTDSGGFQVFSLGELRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQF
DECTPYATGGVPTTHREAAESMRMSLRWAKRSLDEFDRLGNPNALFGIVQGGMFEDLRDESLAGLAALDFHGYAIGGLSV
GEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHRNSLKP
LDERCGCYTCRHFTRGYLHHLHRVGEILGAQLNTIHNLHYYLELMSEIRAAIETHTFDAFRARFAADRARGVEAEPVAPG
EAAAGG

Sequences:

>Translated_406_residues
MTDRRPEPVPTHVPPENGLNFELLTTDGHARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELREMQAQIILGNTFHLW
LRPGLETIGAHGGLHRFMGWDRPILTDSGGFQVFSLGELRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQF
DECTPYATGGVPTTHREAAESMRMSLRWAKRSLDEFDRLGNPNALFGIVQGGMFEDLRDESLAGLAALDFHGYAIGGLSV
GEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHRNSLKP
LDERCGCYTCRHFTRGYLHHLHRVGEILGAQLNTIHNLHYYLELMSEIRAAIETHTFDAFRARFAADRARGVEAEPVAPG
EAAAGG
>Mature_405_residues
TDRRPEPVPTHVPPENGLNFELLTTDGHARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELREMQAQIILGNTFHLWL
RPGLETIGAHGGLHRFMGWDRPILTDSGGFQVFSLGELRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFD
ECTPYATGGVPTTHREAAESMRMSLRWAKRSLDEFDRLGNPNALFGIVQGGMFEDLRDESLAGLAALDFHGYAIGGLSVG
EPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHRNSLKPL
DERCGCYTCRHFTRGYLHHLHRVGEILGAQLNTIHNLHYYLELMSEIRAAIETHTFDAFRARFAADRARGVEAEPVAPGE
AAAGG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=43.6619718309859, Blast_Score=286, Evalue=2e-77,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=64.1711229946524, Blast_Score=488, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17544680, Length=379, Percent_Identity=42.2163588390501, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=164, Percent_Identity=29.2682926829268, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920510, Length=390, Percent_Identity=44.1025641025641, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI21355311, Length=170, Percent_Identity=27.0588235294118, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 45083; Mature: 44952

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRRPEPVPTHVPPENGLNFELLTTDGHARRGRVTLNHGVVETPIFMPVGTYGTVKAVQ
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCCCEECCC
PRELREMQAQIILGNTFHLWLRPGLETIGAHGGLHRFMGWDRPILTDSGGFQVFSLGELR
HHHHHHHHHEEEECCEEEEEECCCHHHHHCCCCHHHHCCCCCCEEECCCCEEEEECHHHH
KITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFDECTPYATGGVPTTHREAAE
HHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHH
SMRMSLRWAKRSLDEFDRLGNPNALFGIVQGGMFEDLRDESLAGLAALDFHGYAIGGLSV
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCEEEEECCCEEECCCCC
GEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWL
CCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEE
FTRFGDVKIRNATHRNSLKPLDERCGCYTCRHFTRGYLHHLHRVGEILGAQLNTIHNLHY
EEEECCEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLELMSEIRAAIETHTFDAFRARFAADRARGVEAEPVAPGEAAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDRRPEPVPTHVPPENGLNFELLTTDGHARRGRVTLNHGVVETPIFMPVGTYGTVKAVQ
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCCCEECCC
PRELREMQAQIILGNTFHLWLRPGLETIGAHGGLHRFMGWDRPILTDSGGFQVFSLGELR
HHHHHHHHHEEEECCEEEEEECCCHHHHHCCCCHHHHCCCCCCEEECCCCEEEEECHHHH
KITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFDECTPYATGGVPTTHREAAE
HHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHH
SMRMSLRWAKRSLDEFDRLGNPNALFGIVQGGMFEDLRDESLAGLAALDFHGYAIGGLSV
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCEEEEECCCEEECCCCC
GEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWL
CCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEE
FTRFGDVKIRNATHRNSLKPLDERCGCYTCRHFTRGYLHHLHRVGEILGAQLNTIHNLHY
EEEECCEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLELMSEIRAAIETHTFDAFRARFAADRARGVEAEPVAPGEAAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA