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The map label for this gene is murE [H]

Identifier: 238026127

GI number: 238026127

Start: 481425

End: 482972

Strand: Direct

Name: murE [H]

Synonym: bglu_1g04570

Alternate gene names: 238026127

Gene position: 481425-482972 (Clockwise)

Preceding gene: 238026126

Following gene: 238026128

Centisome position: 12.32

GC content: 71.83

Gene sequence:

>1548_bases
ATGAGCGCCTTGCGCAGCTCCCACCCGGCGCAGGCGCGCATCGCCGCCGCGCTCGCCTGGCTGCGCGAGCACGCGAAACC
GGGCGCGCAACTGCATGCCGACACGCGCTCGCTGCGGGCCGGCGACGTGTTTCTGGCCTATGCCGTGGATGGCGCCGACA
ATCGCCCGCACCTCGCAAACGCGCTGTCGCGCGGCGCCGCGGCCGTGCTCGTGCAGCCCGAAGGCTATGCCGGGATCGAT
GCGCTCGACCCGGCCGTCGCGCTGGCGGTGCCAGGGCTCGACGCGCTGGCGGGCGAGATCGCGAGCGGCTGGTACGGCGA
TCCGAGCGACGCGATGTTCGTGATCGGCGTGACCGGCACCAACGGCAAGACCTCCTGCACGCAATGGATCGCGACGGCGC
TTACCGCGCTGCGCCGGCCGTGCGCGGTGATCGGCACGCTCGGCAGCGGCATGCCGGGCCGGCTCGTGGCTACCGGTTTC
ACCACGCCCGACGCGCCGCAACTGCAGCGCAGCCTCGCGCAACTGCGCGACGACGGTGCCCAGGCGGTGGCGATGGAGGT
GTCTTCGCACGCGCTGCACCAGGGCCGTGTCAACGGCACCGCCTTCGACGTCGCGGTGTTCACGAACCTGACCCAGGATC
ATCTCGACTATCACCGCACCTTCGAGGCCTACGAGGCGGCGAAGGCAAAGCTGTTCGCCTGGCGCGGGCTGCGCCACGCG
ATCATCAACCGCGACGACGCGGCGGGAGGGCGGCTGCTCGCCACGCTCGCGGGCCGTGTGCGCACCGTGGCCTACGGCAT
CGGCGCGCGCGCCGATGCGCCCGAGGCCGACCGCACGCTGATCGCGAGCGAGGTGCGCGCAACGGCGACCGGCACCGCGT
TTCGGCTGACGTCGTCGTGGGGCGATGCCGACCTCGAGGTCGGCACGCTCGGCCTGTTCAACGTCAGCAACCTGCTGGCC
GTGCTCGCCACGCTGCTGGTGGCCGACCTGCCGTTCGACGCGGCACTTGCCGAGATGCGCCGGCTCGAGTCGGTGAACGG
GCGGATGCAGCGGCTCGGCGGCCGGATCGATCACGACGAGCCGCTGGTGGTGGTCGATTACGCGCACACGCCCGACGCGC
TCGAAAAGACGCTCGAGGCGCTGCGGCCGATCGCCGAAGCGCGTGGCGGCGCGCTGGTCTGCATGTTCGGTTGCGGCGGC
GATCGCGACACGACCAAGCGTCCGCTGATGGGCGCTATTGCCGAGCGGCTCGCCGACCGCGTGGTGGTGACGAGCGACAA
CCCGCGCAGCGAAGCGCCGCTCGCGATCATCGAACAGATCGCGTCCGGCATGCATGCGCCGGACGGCGCGCTGCGCATCG
AGGATCGCGCCAGCGCGATTCTGCAGGCCGTGCGCGGCGCGGCGCGCGAGGACGTGGTGCTGCTGGCGGGGAAGGGACAC
GAGGCCACACAGGAAATCATGGGCAAGAAGCGGCAGTTCTCGGATCAGGATCACGCGCGGCTCGCACTGGCCGCGCGCGC
GGCCAGTGCGCAGGGAGGCGGCGAATGA

Upstream 100 bases:

>100_bases
CCGGCCAGGCCCAGCAGGCTGGCGCGCAGAAGCTGGCGGCCATGCCGGCCGGCCGCATGACCGTGTCGAGCACGACACAC
AGCCGCCCGGGGGTGGTTCG

Downstream 100 bases:

>100_bases
CGATGCTGACGCTGCATGATGCCGCCGCGCTGATTCCGGGCGCCACCGTGATCGGCGACGGCAGCGTCGCGTTCGCGCGC
GTGTCCACCGACAGTCGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSALRSSHPAQARIAAALAWLREHAKPGAQLHADTRSLRAGDVFLAYAVDGADNRPHLANALSRGAAAVLVQPEGYAGID
ALDPAVALAVPGLDALAGEIASGWYGDPSDAMFVIGVTGTNGKTSCTQWIATALTALRRPCAVIGTLGSGMPGRLVATGF
TTPDAPQLQRSLAQLRDDGAQAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRHA
IINRDDAAGGRLLATLAGRVRTVAYGIGARADAPEADRTLIASEVRATATGTAFRLTSSWGDADLEVGTLGLFNVSNLLA
VLATLLVADLPFDAALAEMRRLESVNGRMQRLGGRIDHDEPLVVVDYAHTPDALEKTLEALRPIAEARGGALVCMFGCGG
DRDTTKRPLMGAIAERLADRVVVTSDNPRSEAPLAIIEQIASGMHAPDGALRIEDRASAILQAVRGAAREDVVLLAGKGH
EATQEIMGKKRQFSDQDHARLALAARAASAQGGGE

Sequences:

>Translated_515_residues
MSALRSSHPAQARIAAALAWLREHAKPGAQLHADTRSLRAGDVFLAYAVDGADNRPHLANALSRGAAAVLVQPEGYAGID
ALDPAVALAVPGLDALAGEIASGWYGDPSDAMFVIGVTGTNGKTSCTQWIATALTALRRPCAVIGTLGSGMPGRLVATGF
TTPDAPQLQRSLAQLRDDGAQAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRHA
IINRDDAAGGRLLATLAGRVRTVAYGIGARADAPEADRTLIASEVRATATGTAFRLTSSWGDADLEVGTLGLFNVSNLLA
VLATLLVADLPFDAALAEMRRLESVNGRMQRLGGRIDHDEPLVVVDYAHTPDALEKTLEALRPIAEARGGALVCMFGCGG
DRDTTKRPLMGAIAERLADRVVVTSDNPRSEAPLAIIEQIASGMHAPDGALRIEDRASAILQAVRGAAREDVVLLAGKGH
EATQEIMGKKRQFSDQDHARLALAARAASAQGGGE
>Mature_514_residues
SALRSSHPAQARIAAALAWLREHAKPGAQLHADTRSLRAGDVFLAYAVDGADNRPHLANALSRGAAAVLVQPEGYAGIDA
LDPAVALAVPGLDALAGEIASGWYGDPSDAMFVIGVTGTNGKTSCTQWIATALTALRRPCAVIGTLGSGMPGRLVATGFT
TPDAPQLQRSLAQLRDDGAQAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRHAI
INRDDAAGGRLLATLAGRVRTVAYGIGARADAPEADRTLIASEVRATATGTAFRLTSSWGDADLEVGTLGLFNVSNLLAV
LATLLVADLPFDAALAEMRRLESVNGRMQRLGGRIDHDEPLVVVDYAHTPDALEKTLEALRPIAEARGGALVCMFGCGGD
RDTTKRPLMGAIAERLADRVVVTSDNPRSEAPLAIIEQIASGMHAPDGALRIEDRASAILQAVRGAAREDVVLLAGKGHE
ATQEIMGKKRQFSDQDHARLALAARAASAQGGGE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=470, Percent_Identity=44.0425531914894, Blast_Score=296, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54089; Mature: 53958

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALRSSHPAQARIAAALAWLREHAKPGAQLHADTRSLRAGDVFLAYAVDGADNRPHLAN
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCHHCCCCCCEEEEEEECCCCCCCHHHH
ALSRGAAAVLVQPEGYAGIDALDPAVALAVPGLDALAGEIASGWYGDPSDAMFVIGVTGT
HHCCCCEEEEECCCCCCCCCCCCCHHHEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECC
NGKTSCTQWIATALTALRRPCAVIGTLGSGMPGRLVATGFTTPDAPQLQRSLAQLRDDGA
CCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
QAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRHA
CEEEHHHHHHHHHCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IINRDDAAGGRLLATLAGRVRTVAYGIGARADAPEADRTLIASEVRATATGTAFRLTSSW
HCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
GDADLEVGTLGLFNVSNLLAVLATLLVADLPFDAALAEMRRLESVNGRMQRLGGRIDHDE
CCCCCEECEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLVVVDYAHTPDALEKTLEALRPIAEARGGALVCMFGCGGDRDTTKRPLMGAIAERLADR
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCE
VVVTSDNPRSEAPLAIIEQIASGMHAPDGALRIEDRASAILQAVRGAAREDVVLLAGKGH
EEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCC
EATQEIMGKKRQFSDQDHARLALAARAASAQGGGE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SALRSSHPAQARIAAALAWLREHAKPGAQLHADTRSLRAGDVFLAYAVDGADNRPHLAN
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCHHCCCCCCEEEEEEECCCCCCCHHHH
ALSRGAAAVLVQPEGYAGIDALDPAVALAVPGLDALAGEIASGWYGDPSDAMFVIGVTGT
HHCCCCEEEEECCCCCCCCCCCCCHHHEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECC
NGKTSCTQWIATALTALRRPCAVIGTLGSGMPGRLVATGFTTPDAPQLQRSLAQLRDDGA
CCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
QAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRHA
CEEEHHHHHHHHHCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IINRDDAAGGRLLATLAGRVRTVAYGIGARADAPEADRTLIASEVRATATGTAFRLTSSW
HCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
GDADLEVGTLGLFNVSNLLAVLATLLVADLPFDAALAEMRRLESVNGRMQRLGGRIDHDE
CCCCCEECEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLVVVDYAHTPDALEKTLEALRPIAEARGGALVCMFGCGGDRDTTKRPLMGAIAERLADR
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCE
VVVTSDNPRSEAPLAIIEQIASGMHAPDGALRIEDRASAILQAVRGAAREDVVLLAGKGH
EEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCC
EATQEIMGKKRQFSDQDHARLALAARAASAQGGGE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]