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The map label for this gene is murF [H]

Identifier: 238026128

GI number: 238026128

Start: 482969

End: 484375

Strand: Direct

Name: murF [H]

Synonym: bglu_1g04580

Alternate gene names: 238026128

Gene position: 482969-484375 (Clockwise)

Preceding gene: 238026127

Following gene: 238026129

Centisome position: 12.36

GC content: 73.13

Gene sequence:

>1407_bases
ATGACGATGCTGACGCTGCATGATGCCGCCGCGCTGATTCCGGGCGCCACCGTGATCGGCGACGGCAGCGTCGCGTTCGC
GCGCGTGTCCACCGACAGTCGCACGGTGGGCGAGGGCGACCTGTTCGTCGCGCTGAAGGGCGAGCGCTTCGACGCGCACG
ACTTCCTGGCCGAGGTCGCCGCGCGCGGCGCGAGCGCGGCCCTGGTGTCGCGCGCGGGCGTGCTGCCGGGCCTGCCGCAA
CTGAAGGCGGCCGATACGCGGGTGGCGCTCGGGGCGCTCGCGCACGGCTGGCGGCAGCGCTTCGACCTGCCGCTCGTCGC
CGTCACGGGCAGCAACGGCAAGACCACCGTGAAGGAGATGATCGCGTCGGTGTTCGCGGCCGCGGTCGGCCAGGACGCGC
GGCTTTCCACGGCCGGCAATTTCAACAACGACATCGGTCTGCCATTGACGCTGCTGCGGCTCGCGCCGGCACACCGGCTT
GCCGTGATCGAGCTCGGCATGAATCACCCGGGCGAGACCGGCACGCTCGCCAGGATCGCCGCGCCGACGATTGCGCTCGT
CAATAACGCGCAGCGCGAGCATCAGGAGTTCATGGCGACGGTCGAGGCGGTCGCGCTCGAGCACGCATCGGTGATCCACG
CGCTCGGGCCGGACGGCGTCGCCGTGTTTCCGGCCGACGACCGGTACGCCGGCATCTGGCGCGTGGCGGCCACCGGCAAC
CGGATCGTCGATTTCGCGCTGAACGACGACGGCCGCGCCACGGCGGCCGCGGTAACGGGCACCACGCAGGGCGGCGTGCT
CGCGATCGATACGCCGGCCGGCCGCATCGAGGTGCGGCTGCGCGCGCTCGGCGCGCACAACGCGCGCAACGCGCTGGCGG
CCACGGCCGCAGCGCTCGCGGCAGGCGTCGAGCCCGACGCGATTCGCGCCGGGCTGGAGGCGTTCGAGCCGGTCAAGGGA
CGCCTGCAGGTCAAGCGCGCCTCGCTCGCGTCGCTGGCCGGCGCGATGCTGATCGACGACACCTACAACGCCAATCCCGA
TTCGATGCGCGCCGCAATCGACGTGCTGGCGCAGTGCGCCGCGCCGCGCGTGCTCGTGATCGGCGAGATGGGCGAGGTGG
GCGACGCAGGTCCGGCGTTCCATCGCGAGATCGGCGCCTACGCGCGCGAGCGCGGCATCGACGCGCTCTACGCGACCGGC
GAGGCCACGCGCGAGGCCTGCGCGGCCTACGGCGCGGCGGCGCGCCATTTCGACGAGGTCGGTGCGCTGGTGGCGGCGCT
CGACGCGGCCGGCTACGGTGCGGCGGCGACGGTACTCGTGAAGGGCTCGCGCTACACCCGCATGGAGCGCGTGGTCGACG
CGCTGACGAATCAACCCGCGCCGGGCATCGCGCCCGGCGCTCACTGA

Upstream 100 bases:

>100_bases
CCACACAGGAAATCATGGGCAAGAAGCGGCAGTTCTCGGATCAGGATCACGCGCGGCTCGCACTGGCCGCGCGCGCGGCC
AGTGCGCAGGGAGGCGGCGA

Downstream 100 bases:

>100_bases
GAAGGAAGTCCGCAATGTTGCTGGCGCTGGCGCAATGGCTGCAAGGAGACGCAAGCTTTCTGCGCTTGTTCACGTACCTG
ACGTTCCGCGCGGTGATGGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTMLTLHDAAALIPGATVIGDGSVAFARVSTDSRTVGEGDLFVALKGERFDAHDFLAEVAARGASAALVSRAGVLPGLPQ
LKAADTRVALGALAHGWRQRFDLPLVAVTGSNGKTTVKEMIASVFAAAVGQDARLSTAGNFNNDIGLPLTLLRLAPAHRL
AVIELGMNHPGETGTLARIAAPTIALVNNAQREHQEFMATVEAVALEHASVIHALGPDGVAVFPADDRYAGIWRVAATGN
RIVDFALNDDGRATAAAVTGTTQGGVLAIDTPAGRIEVRLRALGAHNARNALAATAAALAAGVEPDAIRAGLEAFEPVKG
RLQVKRASLASLAGAMLIDDTYNANPDSMRAAIDVLAQCAAPRVLVIGEMGEVGDAGPAFHREIGAYARERGIDALYATG
EATREACAAYGAAARHFDEVGALVAALDAAGYGAAATVLVKGSRYTRMERVVDALTNQPAPGIAPGAH

Sequences:

>Translated_468_residues
MTMLTLHDAAALIPGATVIGDGSVAFARVSTDSRTVGEGDLFVALKGERFDAHDFLAEVAARGASAALVSRAGVLPGLPQ
LKAADTRVALGALAHGWRQRFDLPLVAVTGSNGKTTVKEMIASVFAAAVGQDARLSTAGNFNNDIGLPLTLLRLAPAHRL
AVIELGMNHPGETGTLARIAAPTIALVNNAQREHQEFMATVEAVALEHASVIHALGPDGVAVFPADDRYAGIWRVAATGN
RIVDFALNDDGRATAAAVTGTTQGGVLAIDTPAGRIEVRLRALGAHNARNALAATAAALAAGVEPDAIRAGLEAFEPVKG
RLQVKRASLASLAGAMLIDDTYNANPDSMRAAIDVLAQCAAPRVLVIGEMGEVGDAGPAFHREIGAYARERGIDALYATG
EATREACAAYGAAARHFDEVGALVAALDAAGYGAAATVLVKGSRYTRMERVVDALTNQPAPGIAPGAH
>Mature_467_residues
TMLTLHDAAALIPGATVIGDGSVAFARVSTDSRTVGEGDLFVALKGERFDAHDFLAEVAARGASAALVSRAGVLPGLPQL
KAADTRVALGALAHGWRQRFDLPLVAVTGSNGKTTVKEMIASVFAAAVGQDARLSTAGNFNNDIGLPLTLLRLAPAHRLA
VIELGMNHPGETGTLARIAAPTIALVNNAQREHQEFMATVEAVALEHASVIHALGPDGVAVFPADDRYAGIWRVAATGNR
IVDFALNDDGRATAAAVTGTTQGGVLAIDTPAGRIEVRLRALGAHNARNALAATAAALAAGVEPDAIRAGLEAFEPVKGR
LQVKRASLASLAGAMLIDDTYNANPDSMRAAIDVLAQCAAPRVLVIGEMGEVGDAGPAFHREIGAYARERGIDALYATGE
ATREACAAYGAAARHFDEVGALVAALDAAGYGAAATVLVKGSRYTRMERVVDALTNQPAPGIAPGAH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=430, Percent_Identity=41.6279069767442, Blast_Score=256, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48049; Mature: 47918

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLTLHDAAALIPGATVIGDGSVAFARVSTDSRTVGEGDLFVALKGERFDAHDFLAEVA
CEEEEECCHHHHCCCCEEEECCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
ARGASAALVSRAGVLPGLPQLKAADTRVALGALAHGWRQRFDLPLVAVTGSNGKTTVKEM
HCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
IASVFAAAVGQDARLSTAGNFNNDIGLPLTLLRLAPAHRLAVIELGMNHPGETGTLARIA
HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHH
APTIALVNNAQREHQEFMATVEAVALEHASVIHALGPDGVAVFPADDRYAGIWRVAATGN
CCHHEEHHCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCEEEEEEECCC
RIVDFALNDDGRATAAAVTGTTQGGVLAIDTPAGRIEVRLRALGAHNARNALAATAAALA
EEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHH
AGVEPDAIRAGLEAFEPVKGRLQVKRASLASLAGAMLIDDTYNANPDSMRAAIDVLAQCA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHC
APRVLVIGEMGEVGDAGPAFHREIGAYARERGIDALYATGEATREACAAYGAAARHFDEV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
GALVAALDAAGYGAAATVLVKGSRYTRMERVVDALTNQPAPGIAPGAH
HHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TMLTLHDAAALIPGATVIGDGSVAFARVSTDSRTVGEGDLFVALKGERFDAHDFLAEVA
EEEEECCHHHHCCCCEEEECCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
ARGASAALVSRAGVLPGLPQLKAADTRVALGALAHGWRQRFDLPLVAVTGSNGKTTVKEM
HCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
IASVFAAAVGQDARLSTAGNFNNDIGLPLTLLRLAPAHRLAVIELGMNHPGETGTLARIA
HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHH
APTIALVNNAQREHQEFMATVEAVALEHASVIHALGPDGVAVFPADDRYAGIWRVAATGN
CCHHEEHHCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCEEEEEEECCC
RIVDFALNDDGRATAAAVTGTTQGGVLAIDTPAGRIEVRLRALGAHNARNALAATAAALA
EEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHH
AGVEPDAIRAGLEAFEPVKGRLQVKRASLASLAGAMLIDDTYNANPDSMRAAIDVLAQCA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHC
APRVLVIGEMGEVGDAGPAFHREIGAYARERGIDALYATGEATREACAAYGAAARHFDEV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
GALVAALDAAGYGAAATVLVKGSRYTRMERVVDALTNQPAPGIAPGAH
HHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]