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The map label for this gene is penA [H]

Identifier: 238026126

GI number: 238026126

Start: 479590

End: 481428

Strand: Direct

Name: penA [H]

Synonym: bglu_1g04560

Alternate gene names: 238026126

Gene position: 479590-481428 (Clockwise)

Preceding gene: 238026125

Following gene: 238026127

Centisome position: 12.28

GC content: 66.83

Gene sequence:

>1839_bases
ATGAAGCCGCGTCAGCCGAACGTCAAGTTCGGGTCGAGTCCCGTGCTGTCCGTGCATTTGCCGATGTGGCGCTCGAAGCT
CGTCGTGTTCATGCTTTTCATGGCGTTCTTCGCGCTGGCCGCGCGCGCGTTCTGGATTCAAGGGCCGGGCAACGCGTTCT
ACCAGAAGCAGGGCGAAAGCCGCTACCAGCGCACGCTCGAGCTGCCGGCCACGCGCGGCAAGATTCTCGACCGCAACGGC
CTCGTGCTGGCCACGAGCCTGCCGGTGCGCGCGATCTGGGCGATTCCCGAATCGGTGCCGGACGATCTCGGCGCCGACAA
GCTCGATGAACTCGGCAAGCTGCTCGGCATGAGCACGACCGAGCTGCGCCGCAAGCTGTCCGAGGACAAGAGCTTCGTCT
ACGTGAAGCGGCAGGTGCCGATCGACGTGGCCGACAAGGTGGCCGCGCTCGACATTCCCGGCATCTACGCGCGCAACGAA
TACAAGCGCTTCTACCCGGAAGGGGAGATCACCGCTCACCTGATCGGCTTCACCAACGTCGAGGACGAAGGCCAGGAAGG
CGTCGAGCTCGCCGACCAGAAGCTGCTGGCAGGTGTGCCGGGCAGCCGGCGCGTGATCAAGGACCGGGTGGGCCACATCG
TCGAGGATGTGGCCGAGCAGTTGCCGCCGCACGACGGCACCGACGTGGACCTGTCGATCGACAGCAAGATCCAGTACATC
ACCTACGCCAACCTGAAGGCGGCGATCGAGAAGTTCCACGCCAAGGCGGGCGCGGCGATGGTGGTGGACGTGCGCACCGG
CGAGGTGCTCTCGCTCGTCAATTACCCGACCTACAACCCGAATGACCGTTCGCATCTGACCGGCGAGCAGCTGCGCAACC
GCGCGCTGACCGACGTGTTCGAGCCGGGTTCGATCATGAAGCCGTTCACGGTGTCGCTCGCGCTCGATTTGCATCGCGTC
TCGCCGACCACGCTGGTCGATACCGGCAACGGCCATTTCACGCTCGATGGCGCGCCGATCACCGACGATGCGGGCTTCGG
CGTGCTGACGGTGGGCGGCGTGATCCAGAAGTCGAGCAACATCGGCGCGACCAAGATCGCGATGACGATGCGGCCCGAGG
AAATGTGGAATATGTATACCAGCCTCGGTCTCGGCCAGGCGCCGAAGGTCGGCTTCCCCGGCGCGGCGGCGGGGCGCCTG
CGGCCGTGGAAGAGCTGGCGCCGGATCGAGCAGGCGACGATGTCCTACGGCTACGGCCTGTCGGTCTCGCTGTTCCAGCT
TGCCCACGCCTACACCGCGATCGCCCATGACGGCGAGCTGATGCCGCTGTCGATCTTCAAGGCCAATGAGGCCCAGCCGC
CGCAGGGCCCGCAGGTGTTCTCGCCCACCACCGCGCGCGAAGTGCGCGAGATGCTCGAGAGCGTGGTGACGAAGAACGGC
ACCTCGCCCGACGCAGCGGTCCCGGGTTATCGCGTCGGCGGCAAGAGCGGCACCGCGTACAAGCAGGTCGGCCGCGGCTA
CGACCACAAGAAGTATCGCGCGTCGTTCGTCGGCATGGCGCCGATGCCGAATCCGCGCATCGTGGTGGCCGTGACGGTCG
ACGAGCCGACGGCTGGCAGCCACTTCGGCGGCCAGGTGTCGGGCCCGGTGTTCTCGACGATCGTCGGCGATACGCTGCGC
GCGCTGAACGTGCCGCCGAACATGCCGGTCAAGCAACTCGTGGTGTCCGACGACGCCGGCCAGGCCCAGCAGGCTGGCGC
GCAGAAGCTGGCGGCCATGCCGGCCGGCCGCATGACCGTGTCGAGCACGACACACAGCCGCCCGGGGGTGGTTCGATGA

Upstream 100 bases:

>100_bases
CCGGCCGCACCCAATACCTGGTGCTGGCGCCGGGCGCCGCCAAGGCCGTCGATGCGCCGCTGCCCACCTCGGCCGCGTCG
GGCTCGAAGGGGAGCGCGCG

Downstream 100 bases:

>100_bases
GCGCCTTGCGCAGCTCCCACCCGGCGCAGGCGCGCATCGCCGCCGCGCTCGCCTGGCTGCGCGAGCACGCGAAACCGGGC
GCGCAACTGCATGCCGACAC

Product: Penicillin-binding protein

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MKPRQPNVKFGSSPVLSVHLPMWRSKLVVFMLFMAFFALAARAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILDRNG
LVLATSLPVRAIWAIPESVPDDLGADKLDELGKLLGMSTTELRRKLSEDKSFVYVKRQVPIDVADKVAALDIPGIYARNE
YKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLAGVPGSRRVIKDRVGHIVEDVAEQLPPHDGTDVDLSIDSKIQYI
TYANLKAAIEKFHAKAGAAMVVDVRTGEVLSLVNYPTYNPNDRSHLTGEQLRNRALTDVFEPGSIMKPFTVSLALDLHRV
SPTTLVDTGNGHFTLDGAPITDDAGFGVLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSLGLGQAPKVGFPGAAAGRL
RPWKSWRRIEQATMSYGYGLSVSLFQLAHAYTAIAHDGELMPLSIFKANEAQPPQGPQVFSPTTAREVREMLESVVTKNG
TSPDAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVTVDEPTAGSHFGGQVSGPVFSTIVGDTLR
ALNVPPNMPVKQLVVSDDAGQAQQAGAQKLAAMPAGRMTVSSTTHSRPGVVR

Sequences:

>Translated_612_residues
MKPRQPNVKFGSSPVLSVHLPMWRSKLVVFMLFMAFFALAARAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILDRNG
LVLATSLPVRAIWAIPESVPDDLGADKLDELGKLLGMSTTELRRKLSEDKSFVYVKRQVPIDVADKVAALDIPGIYARNE
YKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLAGVPGSRRVIKDRVGHIVEDVAEQLPPHDGTDVDLSIDSKIQYI
TYANLKAAIEKFHAKAGAAMVVDVRTGEVLSLVNYPTYNPNDRSHLTGEQLRNRALTDVFEPGSIMKPFTVSLALDLHRV
SPTTLVDTGNGHFTLDGAPITDDAGFGVLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSLGLGQAPKVGFPGAAAGRL
RPWKSWRRIEQATMSYGYGLSVSLFQLAHAYTAIAHDGELMPLSIFKANEAQPPQGPQVFSPTTAREVREMLESVVTKNG
TSPDAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVTVDEPTAGSHFGGQVSGPVFSTIVGDTLR
ALNVPPNMPVKQLVVSDDAGQAQQAGAQKLAAMPAGRMTVSSTTHSRPGVVR
>Mature_612_residues
MKPRQPNVKFGSSPVLSVHLPMWRSKLVVFMLFMAFFALAARAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILDRNG
LVLATSLPVRAIWAIPESVPDDLGADKLDELGKLLGMSTTELRRKLSEDKSFVYVKRQVPIDVADKVAALDIPGIYARNE
YKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLAGVPGSRRVIKDRVGHIVEDVAEQLPPHDGTDVDLSIDSKIQYI
TYANLKAAIEKFHAKAGAAMVVDVRTGEVLSLVNYPTYNPNDRSHLTGEQLRNRALTDVFEPGSIMKPFTVSLALDLHRV
SPTTLVDTGNGHFTLDGAPITDDAGFGVLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSLGLGQAPKVGFPGAAAGRL
RPWKSWRRIEQATMSYGYGLSVSLFQLAHAYTAIAHDGELMPLSIFKANEAQPPQGPQVFSPTTAREVREMLESVVTKNG
TSPDAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVTVDEPTAGSHFGGQVSGPVFSTIVGDTLR
ALNVPPNMPVKQLVVSDDAGQAQQAGAQKLAAMPAGRMTVSSTTHSRPGVVR

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=550, Percent_Identity=40.1818181818182, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786854, Length=619, Percent_Identity=24.3941841680129, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 66393; Mature: 66393

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRQPNVKFGSSPVLSVHLPMWRSKLVVFMLFMAFFALAARAFWIQGPGNAFYQKQGES
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCCHH
RYQRTLELPATRGKILDRNGLVLATSLPVRAIWAIPESVPDDLGADKLDELGKLLGMSTT
HHHHHHCCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHH
ELRRKLSEDKSFVYVKRQVPIDVADKVAALDIPGIYARNEYKRFYPEGEITAHLIGFTNV
HHHHHHCCCCCEEEEEEECCCCHHHCEEEECCCCCEECCCHHHHCCCCCEEEEEEEECCC
EDEGQEGVELADQKLLAGVPGSRRVIKDRVGHIVEDVAEQLPPHDGTDVDLSIDSKIQYI
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEE
TYANLKAAIEKFHAKAGAAMVVDVRTGEVLSLVNYPTYNPNDRSHLTGEQLRNRALTDVF
EEHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHC
EPGSIMKPFTVSLALDLHRVSPTTLVDTGNGHFTLDGAPITDDAGFGVLTVGGVIQKSSN
CCCCCCCCEEEEEEEEEEECCCCEEEECCCCEEEECCCCCCCCCCCCEEEECHHHCCCCC
IGATKIAMTMRPEEMWNMYTSLGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYGYGL
CCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
SVSLFQLAHAYTAIAHDGELMPLSIFKANEAQPPQGPQVFSPTTAREVREMLESVVTKNG
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TSPDAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVTVDEPTAGS
CCCCCCCCCEEECCCCCCHHHHHCCCCCCHHHHHHEEECCCCCCCCEEEEEEECCCCCCC
HFGGQVSGPVFSTIVGDTLRALNVPPNMPVKQLVVSDDAGQAQQAGAQKLAAMPAGRMTV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCHHHHHHHHHHHHCCCCCEEE
SSTTHSRPGVVR
ECCCCCCCCCCC
>Mature Secondary Structure
MKPRQPNVKFGSSPVLSVHLPMWRSKLVVFMLFMAFFALAARAFWIQGPGNAFYQKQGES
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCCHH
RYQRTLELPATRGKILDRNGLVLATSLPVRAIWAIPESVPDDLGADKLDELGKLLGMSTT
HHHHHHCCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHH
ELRRKLSEDKSFVYVKRQVPIDVADKVAALDIPGIYARNEYKRFYPEGEITAHLIGFTNV
HHHHHHCCCCCEEEEEEECCCCHHHCEEEECCCCCEECCCHHHHCCCCCEEEEEEEECCC
EDEGQEGVELADQKLLAGVPGSRRVIKDRVGHIVEDVAEQLPPHDGTDVDLSIDSKIQYI
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEE
TYANLKAAIEKFHAKAGAAMVVDVRTGEVLSLVNYPTYNPNDRSHLTGEQLRNRALTDVF
EEHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHC
EPGSIMKPFTVSLALDLHRVSPTTLVDTGNGHFTLDGAPITDDAGFGVLTVGGVIQKSSN
CCCCCCCCEEEEEEEEEEECCCCEEEECCCCEEEECCCCCCCCCCCCEEEECHHHCCCCC
IGATKIAMTMRPEEMWNMYTSLGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYGYGL
CCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
SVSLFQLAHAYTAIAHDGELMPLSIFKANEAQPPQGPQVFSPTTAREVREMLESVVTKNG
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TSPDAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVTVDEPTAGS
CCCCCCCCCEEECCCCCCHHHHHCCCCCCHHHHHHEEECCCCCCCCEEEEEEECCCCCCC
HFGGQVSGPVFSTIVGDTLRALNVPPNMPVKQLVVSDDAGQAQQAGAQKLAAMPAGRMTV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCHHHHHHHHHHHHCCCCCEEE
SSTTHSRPGVVR
ECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]