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The map label for this gene is paaI [H]

Identifier: 238026117

GI number: 238026117

Start: 464794

End: 465273

Strand: Reverse

Name: paaI [H]

Synonym: bglu_1g04470

Alternate gene names: 238026117

Gene position: 465273-464794 (Counterclockwise)

Preceding gene: 238026118

Following gene: 238026116

Centisome position: 11.91

GC content: 72.08

Gene sequence:

>480_bases
ATGAACGCCCCCCGCGCCCCTGGCTCGGCCGCCGATACCGATAACCAGGCCGACCCCGACGCGCTCGCGCGGGCGGCCGC
CGACGCGATGTACGCGGCCGACGCCTGCACCCGCGCGCTCGGCATCGAGCTGCTCGAGGTCGGGCCGGGCCGCGCGCGAA
TGCGCATGGCGGTGCGCGCCGAGTTCCTGAACGGCCACCACACCTGCCACGGCGGGATGATCTTCGCGCTGGCCGATTCC
GCCTTCGCGTTCGCCTGCAATTCGCACAACCTGAATACGGTGGCAGCCGGCTGCTCGATCGAGTTCCTGCGCCCCGTCGC
CTTGAACGAGGTGCTGACGGCCGAGGCGCACGAGCAGACGCTGAGCGGCCGCCACGGCATCTACGATATCCGCGTGACCA
ACCGCGCGGGCGAGACGGTGGCGATGTTCCGCGGCAAGTCGGCCCGGATCGGGGGCACCGTGACCGCGACCGGCGCCTGA

Upstream 100 bases:

>100_bases
GAACGCGACCGCCAGCGCGAACTGGGCCAATCGCACGACTACGCGGAAGGGGTGGCCGCCTTCATCGAGAAGCGGGCGCC
GCGCTTCGAAGGACGCTGAG

Downstream 100 bases:

>100_bases
GCGCGCCGCCGCCAGCGAACAGGACGATGGCAGGAGACACCGATGAACGCTCAACTTGCGCTCGAACCGATCGAGACCGC
CAGCCGCGACGAACTGCAGG

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNAPRAPGSAADTDNQADPDALARAAADAMYAADACTRALGIELLEVGPGRARMRMAVRAEFLNGHHTCHGGMIFALADS
AFAFACNSHNLNTVAAGCSIEFLRPVALNEVLTAEAHEQTLSGRHGIYDIRVTNRAGETVAMFRGKSARIGGTVTATGA

Sequences:

>Translated_159_residues
MNAPRAPGSAADTDNQADPDALARAAADAMYAADACTRALGIELLEVGPGRARMRMAVRAEFLNGHHTCHGGMIFALADS
AFAFACNSHNLNTVAAGCSIEFLRPVALNEVLTAEAHEQTLSGRHGIYDIRVTNRAGETVAMFRGKSARIGGTVTATGA
>Mature_159_residues
MNAPRAPGSAADTDNQADPDALARAAADAMYAADACTRALGIELLEVGPGRARMRMAVRAEFLNGHHTCHGGMIFALADS
AFAFACNSHNLNTVAAGCSIEFLRPVALNEVLTAEAHEQTLSGRHGIYDIRVTNRAGETVAMFRGKSARIGGTVTATGA

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=129, Percent_Identity=51.937984496124, Blast_Score=144, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 16570; Mature: 16570

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPRAPGSAADTDNQADPDALARAAADAMYAADACTRALGIELLEVGPGRARMRMAVRA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHH
EFLNGHHTCHGGMIFALADSAFAFACNSHNLNTVAAGCSIEFLRPVALNEVLTAEAHEQT
HHHCCCEEECCCEEEEEECCEEEEEECCCCCEEEEECCCEEECCCHHHHHHHHHHHHHHH
LSGRHGIYDIRVTNRAGETVAMFRGKSARIGGTVTATGA
CCCCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEECCC
>Mature Secondary Structure
MNAPRAPGSAADTDNQADPDALARAAADAMYAADACTRALGIELLEVGPGRARMRMAVRA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHH
EFLNGHHTCHGGMIFALADSAFAFACNSHNLNTVAAGCSIEFLRPVALNEVLTAEAHEQT
HHHCCCEEECCCEEEEEECCEEEEEECCCCCEEEEECCCEEECCCHHHHHHHHHHHHHHH
LSGRHGIYDIRVTNRAGETVAMFRGKSARIGGTVTATGA
CCCCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]