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The map label for this gene is paaK [H]

Identifier: 238026116

GI number: 238026116

Start: 463456

End: 464751

Strand: Reverse

Name: paaK [H]

Synonym: bglu_1g04460

Alternate gene names: 238026116

Gene position: 464751-463456 (Counterclockwise)

Preceding gene: 238026117

Following gene: 238026113

Centisome position: 11.9

GC content: 68.6

Gene sequence:

>1296_bases
ATGAACGCTCAACTTGCGCTCGAACCGATCGAGACCGCCAGCCGCGACGAACTGCAGGCGCTGCAGCTCGAGCGCCTGCG
GTGGTCGCTCACGCACGCCTATGAGAACTCGCCTGTCTATCGACGCAAGTTCGACGCGGCGGGGGTGCATCCGCACGAGT
TGCGCGGCCTCGCCGATCTCGCGCGGTTCCCGTTCACGACCAAGGACGACCTGCGCGACAACTATCCGTTCGGCATGTTC
GCGGTACCGCCGGAGCGGGTCTCGCGCCTGCACGCCTCGTCCGGCACCACCGGCAAGCCTACCGTGGTCGGCTACACGGC
ACGCGACATCGACACCTGGGCCGGCCTCGTCGCGCGCTCGATCCGTGCGGCCGGGGCGCGACGCGGCGACAAGGTCCACG
TCAGCTACGGCTATGGCCTGTTCACGGGCGGCCTGGGCGCGCATTACGGCGCCGAGCGCGCCGGCCTGACGGTGATCCCG
TTCGGCGGCGGCCAGACCGAGAAGCAGGTGCAGCTGATTCGCGATTTCCGGCCCGACATCATCATGGTGACGCCGAGCTA
CATGCTGTCGATTGCCGACGAGATGGAGCGGCAGGGGCTCGACCCGCGCGAATCGTCGCTGCGGATCGGCATCTTCGGCG
CCGAACCATGGACCAACGAGATGCGCGCGGCGCTCGAGGCCCGCCTGGGTATCGACGCGGTCGACATTTATGGATTGTCG
GAGGTGATCGGGCCGGGAGTCGCGTCCGAATGCGCGGCCACCAAGGACGGCCCGACGGTGTGGGAGGACCACTTCTATCC
GGAGGTGGTGGATCCGCTGACGGGCGAGGTGCTGCCGGACGGCGAACTCGGCGAGCTGGTGTTCACCTCGCTGACCAAGG
AGGCGCTGCCGATCGTGCGCTACCGCACGCGCGATCTCACACGCCTGCTGCCCGGCACGGCGCGCCCGATGCGGCGGATG
GAGAAGATCACCGGCCGTGCCGACGACATGATGATCGTTCGCGGCGTGAACGTGTTTCCGACTCAGATCGAGGAGCAGTT
GCTCAAGCAACGCGCGCTGGCGCCGCATTTCCAGATCGTGCTGACGAAGGATGGCCCGCTCGACGTGATGACGCTGAACG
TGGAGCCCTGCCCGCAAGCGGCGGCCGATGCCGCGCAGCTGGACGCCGCGCGTCGCGCGCTGGCCCACGACATCAAGGCG
CTGATCGGCGTGAGCGCGGTGGTCGAGGTGCTGGCGCCGAACGGCATCGAGCGCTCGGTCGGCAAGGCACGCCGCGTGAT
CGACCGGCGCCGCTGA

Upstream 100 bases:

>100_bases
GATGTTCCGCGGCAAGTCGGCCCGGATCGGGGGCACCGTGACCGCGACCGGCGCCTGAGCGCGCCGCCGCCAGCGAACAG
GACGATGGCAGGAGACACCG

Downstream 100 bases:

>100_bases
AGGCCGCCGCCGGCTCACCCCGCAGGCGCCCCGCCGCGGCGGCCGCGCCTCAGGAGTTCGGCAGCAGCAGCCACATCCGG
CCGACCTGCTTCATGCGGCC

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNAQLALEPIETASRDELQALQLERLRWSLTHAYENSPVYRRKFDAAGVHPHELRGLADLARFPFTTKDDLRDNYPFGMF
AVPPERVSRLHASSGTTGKPTVVGYTARDIDTWAGLVARSIRAAGARRGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIP
FGGGQTEKQVQLIRDFRPDIIMVTPSYMLSIADEMERQGLDPRESSLRIGIFGAEPWTNEMRAALEARLGIDAVDIYGLS
EVIGPGVASECAATKDGPTVWEDHFYPEVVDPLTGEVLPDGELGELVFTSLTKEALPIVRYRTRDLTRLLPGTARPMRRM
EKITGRADDMMIVRGVNVFPTQIEEQLLKQRALAPHFQIVLTKDGPLDVMTLNVEPCPQAAADAAQLDAARRALAHDIKA
LIGVSAVVEVLAPNGIERSVGKARRVIDRRR

Sequences:

>Translated_431_residues
MNAQLALEPIETASRDELQALQLERLRWSLTHAYENSPVYRRKFDAAGVHPHELRGLADLARFPFTTKDDLRDNYPFGMF
AVPPERVSRLHASSGTTGKPTVVGYTARDIDTWAGLVARSIRAAGARRGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIP
FGGGQTEKQVQLIRDFRPDIIMVTPSYMLSIADEMERQGLDPRESSLRIGIFGAEPWTNEMRAALEARLGIDAVDIYGLS
EVIGPGVASECAATKDGPTVWEDHFYPEVVDPLTGEVLPDGELGELVFTSLTKEALPIVRYRTRDLTRLLPGTARPMRRM
EKITGRADDMMIVRGVNVFPTQIEEQLLKQRALAPHFQIVLTKDGPLDVMTLNVEPCPQAAADAAQLDAARRALAHDIKA
LIGVSAVVEVLAPNGIERSVGKARRVIDRRR
>Mature_431_residues
MNAQLALEPIETASRDELQALQLERLRWSLTHAYENSPVYRRKFDAAGVHPHELRGLADLARFPFTTKDDLRDNYPFGMF
AVPPERVSRLHASSGTTGKPTVVGYTARDIDTWAGLVARSIRAAGARRGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIP
FGGGQTEKQVQLIRDFRPDIIMVTPSYMLSIADEMERQGLDPRESSLRIGIFGAEPWTNEMRAALEARLGIDAVDIYGLS
EVIGPGVASECAATKDGPTVWEDHFYPEVVDPLTGEVLPDGELGELVFTSLTKEALPIVRYRTRDLTRLLPGTARPMRRM
EKITGRADDMMIVRGVNVFPTQIEEQLLKQRALAPHFQIVLTKDGPLDVMTLNVEPCPQAAADAAQLDAARRALAHDIKA
LIGVSAVVEVLAPNGIERSVGKARRVIDRRR

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=432, Percent_Identity=64.1203703703704, Blast_Score=573, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 47425; Mature: 47425

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQLALEPIETASRDELQALQLERLRWSLTHAYENSPVYRRKFDAAGVHPHELRGLADL
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHH
ARFPFTTKDDLRDNYPFGMFAVPPERVSRLHASSGTTGKPTVVGYTARDIDTWAGLVARS
HHCCCCCCHHHCCCCCCCEEECCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
IRAAGARRGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIPFGGGQTEKQVQLIRDFRPDI
HHHHCCCCCCEEEEEECCHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCE
IMVTPSYMLSIADEMERQGLDPRESSLRIGIFGAEPWTNEMRAALEARLGIDAVDIYGLS
EEECHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCHH
EVIGPGVASECAATKDGPTVWEDHFYPEVVDPLTGEVLPDGELGELVFTSLTKEALPIVR
HHHCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YRTRDLTRLLPGTARPMRRMEKITGRADDMMIVRGVNVFPTQIEEQLLKQRALAPHFQIV
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
LTKDGPLDVMTLNVEPCPQAAADAAQLDAARRALAHDIKALIGVSAVVEVLAPNGIERSV
EECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GKARRVIDRRR
HHHHHHHHCCC
>Mature Secondary Structure
MNAQLALEPIETASRDELQALQLERLRWSLTHAYENSPVYRRKFDAAGVHPHELRGLADL
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHH
ARFPFTTKDDLRDNYPFGMFAVPPERVSRLHASSGTTGKPTVVGYTARDIDTWAGLVARS
HHCCCCCCHHHCCCCCCCEEECCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
IRAAGARRGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIPFGGGQTEKQVQLIRDFRPDI
HHHHCCCCCCEEEEEECCHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCE
IMVTPSYMLSIADEMERQGLDPRESSLRIGIFGAEPWTNEMRAALEARLGIDAVDIYGLS
EEECHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCHH
EVIGPGVASECAATKDGPTVWEDHFYPEVVDPLTGEVLPDGELGELVFTSLTKEALPIVR
HHHCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YRTRDLTRLLPGTARPMRRMEKITGRADDMMIVRGVNVFPTQIEEQLLKQRALAPHFQIV
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
LTKDGPLDVMTLNVEPCPQAAADAAQLDAARRALAHDIKALIGVSAVVEVLAPNGIERSV
EECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GKARRVIDRRR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]