BacMap

Definition	Burkholderia glumae BGR1 chromosome chromosome 1, complete sequence.
Accession	NC_012724
Length	3,906,507

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The map label for this gene is paaG [H]

Identifier: 238026118

GI number: 238026118

Start: 465275

End: 466066

Strand: Reverse

Name: paaG [H]

Synonym: bglu_1g04480

Alternate gene names: 238026118

Gene position: 466066-465275 (Counterclockwise)

Preceding gene: 238026119

Following gene: 238026117

Centisome position: 11.93

GC content: 71.72

Gene sequence:

>792_bases
ATGTCGCACGATACGATCCGCTTCGAGCTCGACCCGCAGACGCGGGTCGCAACGATCACGCTGAACCGTCCCGACAAGCT
CAACAGCTTCACCCGCGCGATGCACGCGGCGCTGGCCGCCGCGCTCGACGAGGCGGAGGCGGGCCAAGCGCGCGCGCTGG
TGCTGACCGGCGCGGGCCGAGGCTTCTGCGCGGGCCAGGACCTCGCCGATCTCGACTTCACGCCCGGCGCGGCGACCGAT
CTCGGCGCCTTGATCGACACGCACTTCAATCCCCTGGTGCGCCGCCTGCAGGGGCTGCCGCTGCCCGTGATCGCCGCCGT
CAACGGCATCGCGGCCGGCGCCGGCGCCAACCTCGCGCTCGCCTGCGACCTCGTGCTGGCCGCGAAATCGGCCAACTTCA
TCCAGTCGTTCGTGAAGATCGGCCTGGTGCCCGACTCGGGCGGTACCTGGTTCCTGCCGCAGCGCGTCGGCTTCGCGCGC
GCGCTCGGACTCGCGCTGACGGGCGAGAAACTAGGTGCCGAACGCGCGGCCGAGTGGGGGCTGATCTGGCGCGCCGTCGA
CGACGACGCGCTGGCCGCCAGCGCCGCCGCGCTCGCGGCCGACCTCGCGAAGCAGCCGACCCGCGCGATCGCCGCGATCA
AGCAGACCATGCGCGCAGGGCTGACTCAAACGCTCGACCAGCAGCTCGACCTCGAACGCGACCGCCAGCGCGAACTGGGC
CAATCGCACGACTACGCGGAAGGGGTGGCCGCCTTCATCGAGAAGCGGGCGCCGCGCTTCGAAGGACGCTGA

Upstream 100 bases:

>100_bases
CATCCACGTCGCGCCGCAGGCCGACACCGCCGGCGCCTGAGCCATCCCCGCCGCACCGGCCGGCACGGCGCCGGCCGCGC
GTCTCCATGAGGAGATCCCG

Downstream 100 bases:

>100_bases
GATGAACGCCCCCCGCGCCCCTGGCTCGGCCGCCGATACCGATAACCAGGCCGACCCCGACGCGCTCGCGCGGGCGGCCG
CCGACGCGATGTACGCGGCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSHDTIRFELDPQTRVATITLNRPDKLNSFTRAMHAALAAALDEAEAGQARALVLTGAGRGFCAGQDLADLDFTPGAATD
LGALIDTHFNPLVRRLQGLPLPVIAAVNGIAAGAGANLALACDLVLAAKSANFIQSFVKIGLVPDSGGTWFLPQRVGFAR
ALGLALTGEKLGAERAAEWGLIWRAVDDDALAASAAALAADLAKQPTRAIAAIKQTMRAGLTQTLDQQLDLERDRQRELG
QSHDYAEGVAAFIEKRAPRFEGR

Sequences:

>Translated_263_residues
MSHDTIRFELDPQTRVATITLNRPDKLNSFTRAMHAALAAALDEAEAGQARALVLTGAGRGFCAGQDLADLDFTPGAATD
LGALIDTHFNPLVRRLQGLPLPVIAAVNGIAAGAGANLALACDLVLAAKSANFIQSFVKIGLVPDSGGTWFLPQRVGFAR
ALGLALTGEKLGAERAAEWGLIWRAVDDDALAASAAALAADLAKQPTRAIAAIKQTMRAGLTQTLDQQLDLERDRQRELG
QSHDYAEGVAAFIEKRAPRFEGR
>Mature_262_residues
SHDTIRFELDPQTRVATITLNRPDKLNSFTRAMHAALAAALDEAEAGQARALVLTGAGRGFCAGQDLADLDFTPGAATDL
GALIDTHFNPLVRRLQGLPLPVIAAVNGIAAGAGANLALACDLVLAAKSANFIQSFVKIGLVPDSGGTWFLPQRVGFARA
LGLALTGEKLGAERAAEWGLIWRAVDDDALAASAAALAADLAKQPTRAIAAIKQTMRAGLTQTLDQQLDLERDRQRELGQ
SHDYAEGVAAFIEKRAPRFEGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=32, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=27.5303643724696, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=27.8195488721804, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4502327, Length=269, Percent_Identity=28.996282527881, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=204, Percent_Identity=27.9411764705882, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI260275230, Length=204, Percent_Identity=27.9411764705882, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI260274832, Length=204, Percent_Identity=27.9411764705882, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI213417737, Length=223, Percent_Identity=27.8026905829596, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI157694516, Length=223, Percent_Identity=27.8026905829596, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=30.7262569832402, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4757968, Length=242, Percent_Identity=24.3801652892562, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=242, Percent_Identity=24.3801652892562, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI25453481, Length=240, Percent_Identity=24.1666666666667, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538573, Length=240, Percent_Identity=24.1666666666667, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=23.9495798319328, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=23.9495798319328, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=23.9495798319328, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594471, Length=228, Percent_Identity=28.5087719298246, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=228, Percent_Identity=28.5087719298246, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4757966, Length=234, Percent_Identity=24.3589743589744, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538581, Length=234, Percent_Identity=24.3589743589744, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI68989263, Length=156, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=58.4, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=36.5079365079365, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=35.1145038167939, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=28.2352941176471, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788597, Length=274, Percent_Identity=28.8321167883212, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788682, Length=222, Percent_Identity=29.2792792792793, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790281, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=36, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17554946, Length=262, Percent_Identity=31.2977099236641, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17549921, Length=240, Percent_Identity=28.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=28.1632653061224, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=28.6792452830189, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=25.5905511811024, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=262, Percent_Identity=30.1526717557252, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=182, Percent_Identity=32.4175824175824, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=25.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=28.042328042328, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=30.2197802197802, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=30.2197802197802, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=30.2197802197802, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=186, Percent_Identity=29.5698924731183, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=32.3886639676113, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=32.3886639676113, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=31.1023622047244, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=28.3464566929134, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19920382, Length=257, Percent_Identity=29.9610894941634, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24654903, Length=255, Percent_Identity=23.921568627451, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21357171, Length=218, Percent_Identity=28.8990825688073, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650670, Length=203, Percent_Identity=29.064039408867, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27774; Mature: 27643

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDTIRFELDPQTRVATITLNRPDKLNSFTRAMHAALAAALDEAEAGQARALVLTGAGR
CCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GFCAGQDLADLDFTPGAATDLGALIDTHFNPLVRRLQGLPLPVIAAVNGIAAGAGANLAL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHH
ACDLVLAAKSANFIQSFVKIGLVPDSGGTWFLPQRVGFARALGLALTGEKLGAERAAEWG
HHHHHHHHHHHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHHCCCHHHCHHHHHHHC
LIWRAVDDDALAASAAALAADLAKQPTRAIAAIKQTMRAGLTQTLDQQLDLERDRQRELG
EEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
QSHDYAEGVAAFIEKRAPRFEGR
CCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SHDTIRFELDPQTRVATITLNRPDKLNSFTRAMHAALAAALDEAEAGQARALVLTGAGR
CCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GFCAGQDLADLDFTPGAATDLGALIDTHFNPLVRRLQGLPLPVIAAVNGIAAGAGANLAL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHH
ACDLVLAAKSANFIQSFVKIGLVPDSGGTWFLPQRVGFARALGLALTGEKLGAERAAEWG
HHHHHHHHHHHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHHCCCHHHCHHHHHHHC
LIWRAVDDDALAASAAALAADLAKQPTRAIAAIKQTMRAGLTQTLDQQLDLERDRQRELG
EEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
QSHDYAEGVAAFIEKRAPRFEGR
CCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]