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The map label for this gene is hisF [H]

Identifier: 238026011

GI number: 238026011

Start: 356035

End: 356808

Strand: Direct

Name: hisF [H]

Synonym: bglu_1g03370

Alternate gene names: 238026011

Gene position: 356035-356808 (Clockwise)

Preceding gene: 238026010

Following gene: 238026012

Centisome position: 9.11

GC content: 70.28

Gene sequence:

>774_bases
ATGGCTCTAGCTAAACGCATCATCCCCTGTCTGGACGTGACCGCCGGGCGCGTCGTGAAAGGCGTCAATTTCGTCGAGCT
GCGCGACGCGGGCGACCCCGTCGAAATCGCGCGGCGCTACGACGACCAGGGCGCCGACGAACTGACGTTCCTCGACATCA
CCGCCACCTCGGACCAGCGCGACCTGATCCTGCCGATCATCGAGGCGGTGGCCTCGCAGGTCTTCATCCCGCTGACGGTG
GGCGGCGGCGTGCGGGCGGTCGAGGACGTGCGGCGCCTGCTCAACGCCGGCGCGGACAAGATCAGCATGAATTCCTCGGC
GGTCGCCAACCCGCAGCTGGTCGCCGACGCGGCCGGCAAGTACGGCTCGCAATGCATCGTGGTGGCGATCGACGCCAAGC
GGGTTTCGGCCGAGGGCGAGACGCCGCGCTGGGAAGTGTTCACGCACGGCGGGCGCAAGGCCACCGGGCTCGACGCGATC
GCGTGGGCGCGCCGCATGGCCGAGCTCGGCGCGGGCGAGATCCTGCTGACCAGCATGGACCGCGACGGCACCAAGTCCGG
CTTCGACCTGGCGCTCACGCGCGGCGTGTCGGACGCCGTACCGGTGCCGGTGATCGCCTCCGGCGGCGTGGGCTCGCTCG
AGCACCTGGCCGACGGCATCGTCGAGGGCCATGCCGACGCGGTGCTCGCGGCCAGCATCTTCCATTACGGCGAGCATACG
GTCGGCGAGGCGAAGCGCTTCATGGCCGGGCGCGGCATTCCGGTGAGGCTCTGA

Upstream 100 bases:

>100_bases
GCTCGACGACGCCTGAGCCGCGGCGGCCCAGCGCCGCGTGCGTCATCGCCCAAGCCGCCCGCGCCGCGGGCGGCCTCACC
TGCGGCACCTGTGTACCATC

Downstream 100 bases:

>100_bases
TCATGACGACGACACAGGCGGCGGCCCGCCCCGCGTGGCTCGACAAGGTCCGCTGGGACGCCAACGGCCTCGTGCCGGTG
ATCGCGCAGGAGGTCGGCAC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKISMNSSAVANPQLVADAAGKYGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKATGLDAI
AWARRMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLEHLADGIVEGHADAVLAASIFHYGEHT
VGEAKRFMAGRGIPVRL

Sequences:

>Translated_257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKISMNSSAVANPQLVADAAGKYGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKATGLDAI
AWARRMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLEHLADGIVEGHADAVLAASIFHYGEHT
VGEAKRFMAGRGIPVRL
>Mature_256_residues
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKISMNSSAVANPQLVADAAGKYGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKATGLDAIA
WARRMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLEHLADGIVEGHADAVLAASIFHYGEHTV
GEAKRFMAGRGIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=42.4710424710425, Blast_Score=210, Evalue=6e-56,
Organism=Escherichia coli, GI87082028, Length=215, Percent_Identity=31.6279069767442, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27010; Mature: 26879

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQR
CCCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKISMNSSAVANPQLVADAAGK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCC
YGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKATGLDAIAWARRMAELGAGEILLTSMD
CCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLEHLADGIVEGHADAVLAASIFHYGEHT
CCCCCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
VGEAKRFMAGRGIPVRL
HHHHHHHHHCCCCEECC
>Mature Secondary Structure 
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQR
CCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKISMNSSAVANPQLVADAAGK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCC
YGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKATGLDAIAWARRMAELGAGEILLTSMD
CCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLEHLADGIVEGHADAVLAASIFHYGEHT
CCCCCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
VGEAKRFMAGRGIPVRL
HHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA