Click here to switch to the map view.

The map label for this gene is thiG [H]

Identifier: 238025992

GI number: 238025992

Start: 339915

End: 340730

Strand: Direct

Name: thiG [H]

Synonym: bglu_1g03180

Alternate gene names: 238025992

Gene position: 339915-340730 (Clockwise)

Preceding gene: 238025991

Following gene: 238025993

Centisome position: 8.7

GC content: 70.96

Gene sequence:

>816_bases
ATGACGCTGCGTTCCCCCACCGATTCGCTGACGCTGTACGGCACCGTGCTGCAAAGCCGCGTGCTGCTCGGCACCTCCCG
TTATCCGTCGCTGCAGTCGCTGTCCGATTCGATCGCCGCGTCGCGGCCCGGCATGGTCACCGTCGCGCTGCGCCGGCAGA
TGAACGCCGGCACCGCCGAGGCCGGCTTCTTCGACCTGCTGCGGCGCCACGAGGTGCCGCTGCTGCCGAACACGGCCGGC
TGCCAGACCGTCGCCGAGGCGGTCAGCACCGCCCAGATGGCGCGCGAGGTGTTCGAAACCGACTGGATCAAGCTCGAACT
GATCGGCGACGACTACACGCTGCAACCCGATCCGGTCGGCCTGATCGAGGCGGCCGCGCTGCTCGTCAAGGACGGCTTCA
AGGTGCTGCCGTACTGCACCGAGGATCTCGTGATCGGCCGGCGGCTGCTCGATGCCGGCTGCGAGGCGCTGATGCCGTGG
GGCGCGCCGATCGGCACCGGCAAGGGCGTGATGAACCCCTACGCGCTGCGCGTGCTGCGCGAGCGGCTGCCGGACGTGCC
GCTGATCGTCGACGCCGGGCTCGGCGTGCCGTCGCACGCGTGCCAGGTGATGGAATGGGGCTTCGACGGCGTGCTGCTGA
ACACGGCCGTCTCGCAGGCCACCCATCCCGAGAGGATGGCGCGCGCCTTCGCGCAGGCCGTCGAGGCGGGCCGCGACGCC
TGGCTGGCCGGGCCGATGGACCCGCGCGAGACCGCTCACGCCAGCACGCCGGTAGTCGGCATGCCGTTCTGGCACCACGA
CGGAGGTGGGCAATGA

Upstream 100 bases:

>100_bases
GCTCGCGGCCGGCGACCGGCTCGACGTCGTGCATCCCGTCGCGGGCGGCTGACCGGCGCGGCCCGCTTGCCTGCCCGGCA
GACCTTTCACAGGACTTCCC

Downstream 100 bases:

>100_bases
GCACGGGCTTCGGTGATATGTTCTGGCCCCCGGTGGATGACCTGGCGGCGGCCGCCGAGCGGATTCGCGCGCGGCTCGGC
GAGTGGCCCGTGCGCCGCGC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTLRSPTDSLTLYGTVLQSRVLLGTSRYPSLQSLSDSIAASRPGMVTVALRRQMNAGTAEAGFFDLLRRHEVPLLPNTAG
CQTVAEAVSTAQMAREVFETDWIKLELIGDDYTLQPDPVGLIEAAALLVKDGFKVLPYCTEDLVIGRRLLDAGCEALMPW
GAPIGTGKGVMNPYALRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPERMARAFAQAVEAGRDA
WLAGPMDPRETAHASTPVVGMPFWHHDGGGQ

Sequences:

>Translated_271_residues
MTLRSPTDSLTLYGTVLQSRVLLGTSRYPSLQSLSDSIAASRPGMVTVALRRQMNAGTAEAGFFDLLRRHEVPLLPNTAG
CQTVAEAVSTAQMAREVFETDWIKLELIGDDYTLQPDPVGLIEAAALLVKDGFKVLPYCTEDLVIGRRLLDAGCEALMPW
GAPIGTGKGVMNPYALRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPERMARAFAQAVEAGRDA
WLAGPMDPRETAHASTPVVGMPFWHHDGGGQ
>Mature_270_residues
TLRSPTDSLTLYGTVLQSRVLLGTSRYPSLQSLSDSIAASRPGMVTVALRRQMNAGTAEAGFFDLLRRHEVPLLPNTAGC
QTVAEAVSTAQMAREVFETDWIKLELIGDDYTLQPDPVGLIEAAALLVKDGFKVLPYCTEDLVIGRRLLDAGCEALMPWG
APIGTGKGVMNPYALRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPERMARAFAQAVEAGRDAW
LAGPMDPRETAHASTPVVGMPFWHHDGGGQ

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=41.6666666666667, Blast_Score=187, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 29174; Mature: 29043

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRSPTDSLTLYGTVLQSRVLLGTSRYPSLQSLSDSIAASRPGMVTVALRRQMNAGTAE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEHEEHHHCCCCCCH
AGFFDLLRRHEVPLLPNTAGCQTVAEAVSTAQMAREVFETDWIKLELIGDDYTLQPDPVG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCH
LIEAAALLVKDGFKVLPYCTEDLVIGRRLLDAGCEALMPWGAPIGTGKGVMNPYALRVLR
HHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
ERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPERMARAFAQAVEAGRDA
HHCCCCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
WLAGPMDPRETAHASTPVVGMPFWHHDGGGQ
EECCCCCHHHHHCCCCCEECCCCEECCCCCC
>Mature Secondary Structure 
TLRSPTDSLTLYGTVLQSRVLLGTSRYPSLQSLSDSIAASRPGMVTVALRRQMNAGTAE
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEHEEHHHCCCCCCH
AGFFDLLRRHEVPLLPNTAGCQTVAEAVSTAQMAREVFETDWIKLELIGDDYTLQPDPVG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCH
LIEAAALLVKDGFKVLPYCTEDLVIGRRLLDAGCEALMPWGAPIGTGKGVMNPYALRVLR
HHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
ERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPERMARAFAQAVEAGRDA
HHCCCCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
WLAGPMDPRETAHASTPVVGMPFWHHDGGGQ
EECCCCCHHHHHCCCCCEECCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA