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The map label for this gene is thiE [H]

Identifier: 238025993

GI number: 238025993

Start: 340727

End: 341824

Strand: Direct

Name: thiE [H]

Synonym: bglu_1g03190

Alternate gene names: 238025993

Gene position: 340727-341824 (Clockwise)

Preceding gene: 238025992

Following gene: 238025994

Centisome position: 8.72

GC content: 76.23

Gene sequence:

>1098_bases
ATGAGCACGGGCTTCGGTGATATGTTCTGGCCCCCGGTGGATGACCTGGCGGCGGCCGCCGAGCGGATTCGCGCGCGGCT
CGGCGAGTGGCCCGTGCGCCGCGCGCCGATGCGAATCTGCGTCGCGGCTCCCGAACGTGCGCAGCCGGGCGACCTGCTGG
TGGTGACGTCGGGCGACGCCGAGGCCGGGCGCGCGGCCGAGGTGATCGCGGCCGGCGGCGCCGCGCTCGAGATCGACGAG
CGGCACGCCACGCTGACGCACGCCGGTGCGCGGTACGCGCTGACCGCGGCCGCGCCGCTCGCCGACGACTGGATCGCGGC
GCTGGCCGCGTTCGCCGACTGTGGATTCGAAGCGCACGACGCGCTGGTGCTGGCGCTTGCCTGGCGTGACGGCGACGAGG
CCGCCGACGGGGATCCCTGGCCGACCGACCCGGCCCGCTTTCCGTCGATCGCCGCTGCGCCGGGTGCGCCCGAGCCGGCC
TTCGCGCCGTGCCCGGCGCGGCTCGGCCTGTATCCGGTGGTGCCGTCGGCCGAATGGGTCGAACGCGTGCTCGACTACGG
CGCGCGCACCGTGCAGCTGCGCGTGAAGGATGCCGCGCCAAACGCGCTGGCCGCCGAGATCGCGCGCGCGGTGGCGGCGG
GGCGCCGCTACCCGGAGGCGCGCGTGTTCATCAACGACCACTGGCGGCTCGCGATCGAGGCCGGCGCCTACGGCGTGCAT
CTCGGCCAGGAGGATCTGCAGGAGGCCGGGCTTGGCGCGATCGCGGCGGCCGGCCTGCGGCTCGGCCTGTCGAGCCATGG
CTACTACGAGATGCTGGTCGCGCTGCGCCTGCGCCCCAGCTATTTGGCGCTCGGCCCGGTGTTCGCCACCGCCACGAAGG
CGGTCGCTGCGCCGCCGCAGGGCCTCGCGCGGCTCGCCCGCTACGCGCGCTTTGCCGGCCGGCGCGCGCCGCTCGTCGCG
ATCGGCGGCGTGACGGCCGCCAGCCTGCCCGAGGTGATCGCCGCCGGCGTCGGCAGCGTGGCCGTGGTCAGCGCGATCAC
GCAGGCCGCGGACCCGCGTGCCGCGGTGGGCGCGCTCGCCGCTTGCTTCGAACGCTGA

Upstream 100 bases:

>100_bases
GCGACGCCTGGCTGGCCGGGCCGATGGACCCGCGCGAGACCGCTCACGCCAGCACGCCGGTAGTCGGCATGCCGTTCTGG
CACCACGACGGAGGTGGGCA

Downstream 100 bases:

>100_bases
AACGAAGCGTTGCGCCCGCGCGCCCGAGGCGCGGGCCTCGGGCCGGGCTGGCCAGGGGCGGCAGGGGCCGCTAACGGGGC
GGTCGTCCGTCCCGGCGAGC

Product: Thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSTGFGDMFWPPVDDLAAAAERIRARLGEWPVRRAPMRICVAAPERAQPGDLLVVTSGDAEAGRAAEVIAAGGAALEIDE
RHATLTHAGARYALTAAAPLADDWIAALAAFADCGFEAHDALVLALAWRDGDEAADGDPWPTDPARFPSIAAAPGAPEPA
FAPCPARLGLYPVVPSAEWVERVLDYGARTVQLRVKDAAPNALAAEIARAVAAGRRYPEARVFINDHWRLAIEAGAYGVH
LGQEDLQEAGLGAIAAAGLRLGLSSHGYYEMLVALRLRPSYLALGPVFATATKAVAAPPQGLARLARYARFAGRRAPLVA
IGGVTAASLPEVIAAGVGSVAVVSAITQAADPRAAVGALAACFER

Sequences:

>Translated_365_residues
MSTGFGDMFWPPVDDLAAAAERIRARLGEWPVRRAPMRICVAAPERAQPGDLLVVTSGDAEAGRAAEVIAAGGAALEIDE
RHATLTHAGARYALTAAAPLADDWIAALAAFADCGFEAHDALVLALAWRDGDEAADGDPWPTDPARFPSIAAAPGAPEPA
FAPCPARLGLYPVVPSAEWVERVLDYGARTVQLRVKDAAPNALAAEIARAVAAGRRYPEARVFINDHWRLAIEAGAYGVH
LGQEDLQEAGLGAIAAAGLRLGLSSHGYYEMLVALRLRPSYLALGPVFATATKAVAAPPQGLARLARYARFAGRRAPLVA
IGGVTAASLPEVIAAGVGSVAVVSAITQAADPRAAVGALAACFER
>Mature_364_residues
STGFGDMFWPPVDDLAAAAERIRARLGEWPVRRAPMRICVAAPERAQPGDLLVVTSGDAEAGRAAEVIAAGGAALEIDER
HATLTHAGARYALTAAAPLADDWIAALAAFADCGFEAHDALVLALAWRDGDEAADGDPWPTDPARFPSIAAAPGAPEPAF
APCPARLGLYPVVPSAEWVERVLDYGARTVQLRVKDAAPNALAAEIARAVAAGRRYPEARVFINDHWRLAIEAGAYGVHL
GQEDLQEAGLGAIAAAGLRLGLSSHGYYEMLVALRLRPSYLALGPVFATATKAVAAPPQGLARLARYARFAGRRAPLVAI
GGVTAASLPEVIAAGVGSVAVVSAITQAADPRAAVGALAACFER

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=203, Percent_Identity=59.6059113300493, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 37824; Mature: 37693

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGFGDMFWPPVDDLAAAAERIRARLGEWPVRRAPMRICVAAPERAQPGDLLVVTSGDA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
EAGRAAEVIAAGGAALEIDERHATLTHAGARYALTAAAPLADDWIAALAAFADCGFEAHD
CCCCHHHHEECCCCEEEECCCHHEHECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCC
ALVLALAWRDGDEAADGDPWPTDPARFPSIAAAPGAPEPAFAPCPARLGLYPVVPSAEWV
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEECCCCHHHH
ERVLDYGARTVQLRVKDAAPNALAAEIARAVAAGRRYPEARVFINDHWRLAIEAGAYGVH
HHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCEEEE
LGQEDLQEAGLGAIAAAGLRLGLSSHGYYEMLVALRLRPSYLALGPVFATATKAVAAPPQ
CCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
GLARLARYARFAGRRAPLVAIGGVTAASLPEVIAAGVGSVAVVSAITQAADPRAAVGALA
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
ACFER
HHHCC
>Mature Secondary Structure 
STGFGDMFWPPVDDLAAAAERIRARLGEWPVRRAPMRICVAAPERAQPGDLLVVTSGDA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
EAGRAAEVIAAGGAALEIDERHATLTHAGARYALTAAAPLADDWIAALAAFADCGFEAHD
CCCCHHHHEECCCCEEEECCCHHEHECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCC
ALVLALAWRDGDEAADGDPWPTDPARFPSIAAAPGAPEPAFAPCPARLGLYPVVPSAEWV
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEECCCCHHHH
ERVLDYGARTVQLRVKDAAPNALAAEIARAVAAGRRYPEARVFINDHWRLAIEAGAYGVH
HHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCEEEE
LGQEDLQEAGLGAIAAAGLRLGLSSHGYYEMLVALRLRPSYLALGPVFATATKAVAAPPQ
CCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
GLARLARYARFAGRRAPLVAIGGVTAASLPEVIAAGVGSVAVVSAITQAADPRAAVGALA
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
ACFER
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA