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The map label for this gene is agaA [H]

Identifier: 238025407

GI number: 238025407

Start: 2593997

End: 2596120

Strand: Direct

Name: agaA [H]

Synonym: bglu_2g20960

Alternate gene names: 238025407

Gene position: 2593997-2596120 (Clockwise)

Preceding gene: 238025405

Following gene: 238025414

Centisome position: 91.75

GC content: 66.48

Gene sequence:

>2124_bases
ATGTCACTGGCATTGTCGGGTTGTGGAGGCGATTCGACGGATGGCAGTGGCGCGGCATCGCTGATGGGCGCAAAGAACAC
TGCCGCCGCGGCAGGCGCCCAGGCGGACGCGCAGCGATCGAGCCAGCCTGGCGCCGGCTCCAGGCAGATGGCGGTCGTGC
GGCCGCCGATGGGCTGGTCGTCATGGAACAGCCTCGAGGAGAACGTCAACTACAACACCATCAAGGCCGAAGCCGACGGG
CTTGCCGCGCTGAACGCACAGATCAAGAGCGGCGCGAAATACGAATACGTCAACACCGACGAAGGCTGGTGGACGTCCGG
CGTACGCGATGCCGACGGCAATTTCGTGATCGACAACACGCAATGGCCCGGCGGCATGCAGGCCATCGTGCAATACATCC
ACAGCAAGGGCCTGAAAGCCGGCATCTACATCGACGCCGGTCCGCAAGGCTGCGGCACGCGCACGGACGGCTCGCACTTC
GTCGGCAGCGACTTCGCGCACTACGATCACGATTTCCTGCAGTTCGCGCAATGGGGCTACGACTTCGTGAAGGTCGATTT
CTGCGGCGGCAGCGCCGCGGGCTACGATCCCCAGCAGGCCTATACGGCCGTCGCTTCGGCGATCGAGAAGGCCTACGTGC
AAACCGGCCGGCGTCTCACGCTGAGTATCTGCGACTGGGGCACGATCGCCGGCAATCCCGCCTATCCCGACTACAAGCAG
GGACCGTGGGCATGGGCCGCCGGCGTCGGCCGCATGTGGCGCACGACGGGCGACATCTACGTTCCCAACACCGGCGCACC
GAGCTACGATCGCGTGCTTGGCAACTTCTTCGGCAACTATCACCCTGAAAGTCAGCACACCGGTTTCTATAACGATCCGG
ACATGATGGTCGCCGGCATGGGGATGACGGCGGTTCAGGATCAGGCGCACATGAGCCTGTGGGCGATCGCGGGCGCACCG
CTGATTCTCGGCAACGATCTTTCGAAGCCGCTCACCGCCGACGCCACGAACCTGATGACGAACAGCGGCGTGATTGCCGT
CGATCAGGATCCGCTTGGCATACAAGGCGTGCTGGTCGCGCAGTCCGGCGCGCAGCAGGTGTGGGCGAAGCTGCTCGCAG
GCAGCGGCCAGCGCGCGGTGGTGCTGTTCAACAACGGCACGAGCGATGCACCGATGACGGTCACCTGGCAGCAGCTCGGC
TTCGCGCCGAACAGCAGCGCGACGGTCCGCGACCTGTGGGCGGGCAAGGACCTTGGATCGTTCACGACGTCCTATACGGC
GCCGACGGTGCCCGCGGGCGGTGCCGTGATGCTCGCGATCAGCGGCAGCGACGTAGCATCGACGGTCTACGAGCCGACCG
CGCTCGGCGGTGGCGCCTCGTACACGCGCTGCTCGGATTGCGCCGGCGGGAAGATCGTGCACGGCCTCGGCACGGTGACG
TTCGGCAACATCGCGGCCGCGAACAACAGCGGCGGCTATGTCGAGATCACGTACGCGAACCGCACGCACGAAACGCTGAC
CGCGCAATTGACGGCCAACGGCGGCCAACCGACCATGGTCGCGTTCCCGCCGACGGGCCGCGACGGCAAGGTCGGCACGG
TCACGCTGTACGTGCCGTTCACGGCAGGCCAGAATTCGATCTCGATTTCGAGCGCCGACAGCACCGCGCCGGCACCGGAG
ATCGCCACGCTCGCGGTGGTGGCCGGCCCGGTCCGGCTGCCGCCGTTCCATGCCGCCTACGAAGCCGAAGCGTCGAACAA
CGTGCTCGCCGGCGGCGCGCGCACCGCGTCGTGCAGCGCATGTTCGGGTGGCGCCGACGTGGGCTATATCGGCAACGGCG
GCACGCTGACGATGAACGGCATCACGGCCGCGGCGGACGGTAAGTACACCGTGCTCATCGGCTACGCGAACGGCGACTCC
GCCCCTCGCTCCGCTGAAATCAGCTTCAACGGCACCACGCCCGTTACCGTCTCGTTTCCGCCGACCGGCGGATGGAACAC
GATCTCGACGCTCGCCGTCACCGGCACGTTCAAGTCGGGCAGCGCCAACACGCTGATCTTCTCGAACTCGTCGGGCTGGG
CGCCGGACATCGACGGCATCGGCACGCCGGTCGCAGCGAACTGA

Upstream 100 bases:

>100_bases
ATTACGCCTTCCGGAACCCCGGAGTTAAACGGAGACTGACATGAATCTGCTCGTTTACAGAAGTCGTGCGTTAATGCTGT
TGGCCGGAATGGCCCTGAGT

Downstream 100 bases:

>100_bases
GCACGCCTCAGGCCGCCCCGACGCGGCGACGGCATCGGCTGTCGCCGCGCCACCAGTTTCTGCAATGCGAGGCTTGAAGG
ACTCCTGCGTAACGTGGTGC

Product: Secreted alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 707; Mature: 706

Protein sequence:

>707_residues
MSLALSGCGGDSTDGSGAASLMGAKNTAAAAGAQADAQRSSQPGAGSRQMAVVRPPMGWSSWNSLEENVNYNTIKAEADG
LAALNAQIKSGAKYEYVNTDEGWWTSGVRDADGNFVIDNTQWPGGMQAIVQYIHSKGLKAGIYIDAGPQGCGTRTDGSHF
VGSDFAHYDHDFLQFAQWGYDFVKVDFCGGSAAGYDPQQAYTAVASAIEKAYVQTGRRLTLSICDWGTIAGNPAYPDYKQ
GPWAWAAGVGRMWRTTGDIYVPNTGAPSYDRVLGNFFGNYHPESQHTGFYNDPDMMVAGMGMTAVQDQAHMSLWAIAGAP
LILGNDLSKPLTADATNLMTNSGVIAVDQDPLGIQGVLVAQSGAQQVWAKLLAGSGQRAVVLFNNGTSDAPMTVTWQQLG
FAPNSSATVRDLWAGKDLGSFTTSYTAPTVPAGGAVMLAISGSDVASTVYEPTALGGGASYTRCSDCAGGKIVHGLGTVT
FGNIAAANNSGGYVEITYANRTHETLTAQLTANGGQPTMVAFPPTGRDGKVGTVTLYVPFTAGQNSISISSADSTAPAPE
IATLAVVAGPVRLPPFHAAYEAEASNNVLAGGARTASCSACSGGADVGYIGNGGTLTMNGITAAADGKYTVLIGYANGDS
APRSAEISFNGTTPVTVSFPPTGGWNTISTLAVTGTFKSGSANTLIFSNSSGWAPDIDGIGTPVAAN

Sequences:

>Translated_707_residues
MSLALSGCGGDSTDGSGAASLMGAKNTAAAAGAQADAQRSSQPGAGSRQMAVVRPPMGWSSWNSLEENVNYNTIKAEADG
LAALNAQIKSGAKYEYVNTDEGWWTSGVRDADGNFVIDNTQWPGGMQAIVQYIHSKGLKAGIYIDAGPQGCGTRTDGSHF
VGSDFAHYDHDFLQFAQWGYDFVKVDFCGGSAAGYDPQQAYTAVASAIEKAYVQTGRRLTLSICDWGTIAGNPAYPDYKQ
GPWAWAAGVGRMWRTTGDIYVPNTGAPSYDRVLGNFFGNYHPESQHTGFYNDPDMMVAGMGMTAVQDQAHMSLWAIAGAP
LILGNDLSKPLTADATNLMTNSGVIAVDQDPLGIQGVLVAQSGAQQVWAKLLAGSGQRAVVLFNNGTSDAPMTVTWQQLG
FAPNSSATVRDLWAGKDLGSFTTSYTAPTVPAGGAVMLAISGSDVASTVYEPTALGGGASYTRCSDCAGGKIVHGLGTVT
FGNIAAANNSGGYVEITYANRTHETLTAQLTANGGQPTMVAFPPTGRDGKVGTVTLYVPFTAGQNSISISSADSTAPAPE
IATLAVVAGPVRLPPFHAAYEAEASNNVLAGGARTASCSACSGGADVGYIGNGGTLTMNGITAAADGKYTVLIGYANGDS
APRSAEISFNGTTPVTVSFPPTGGWNTISTLAVTGTFKSGSANTLIFSNSSGWAPDIDGIGTPVAAN
>Mature_706_residues
SLALSGCGGDSTDGSGAASLMGAKNTAAAAGAQADAQRSSQPGAGSRQMAVVRPPMGWSSWNSLEENVNYNTIKAEADGL
AALNAQIKSGAKYEYVNTDEGWWTSGVRDADGNFVIDNTQWPGGMQAIVQYIHSKGLKAGIYIDAGPQGCGTRTDGSHFV
GSDFAHYDHDFLQFAQWGYDFVKVDFCGGSAAGYDPQQAYTAVASAIEKAYVQTGRRLTLSICDWGTIAGNPAYPDYKQG
PWAWAAGVGRMWRTTGDIYVPNTGAPSYDRVLGNFFGNYHPESQHTGFYNDPDMMVAGMGMTAVQDQAHMSLWAIAGAPL
ILGNDLSKPLTADATNLMTNSGVIAVDQDPLGIQGVLVAQSGAQQVWAKLLAGSGQRAVVLFNNGTSDAPMTVTWQQLGF
APNSSATVRDLWAGKDLGSFTTSYTAPTVPAGGAVMLAISGSDVASTVYEPTALGGGASYTRCSDCAGGKIVHGLGTVTF
GNIAAANNSGGYVEITYANRTHETLTAQLTANGGQPTMVAFPPTGRDGKVGTVTLYVPFTAGQNSISISSADSTAPAPEI
ATLAVVAGPVRLPPFHAAYEAEASNNVLAGGARTASCSACSGGADVGYIGNGGTLTMNGITAAADGKYTVLIGYANGDSA
PRSAEISFNGTTPVTVSFPPTGGWNTISTLAVTGTFKSGSANTLIFSNSSGWAPDIDGIGTPVAAN

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4504009, Length=364, Percent_Identity=30.7692307692308, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4557781, Length=398, Percent_Identity=28.1407035175879, Blast_Score=133, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17562946, Length=426, Percent_Identity=27.6995305164319, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24583253, Length=413, Percent_Identity=29.7820823244552, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19922382, Length=400, Percent_Identity=29.25, Blast_Score=140, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24654142, Length=400, Percent_Identity=29.25, Blast_Score=140, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 72673; Mature: 72542

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLALSGCGGDSTDGSGAASLMGAKNTAAAAGAQADAQRSSQPGAGSRQMAVVRPPMGWS
CCEEEECCCCCCCCCCCCEEEECCCCCHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCC
SWNSLEENVNYNTIKAEADGLAALNAQIKSGAKYEYVNTDEGWWTSGVRDADGNFVIDNT
CHHHHHHCCCCEEEEECCCCEEEEEHHHCCCCEEEEEECCCCCEECCEEECCCCEEEECC
QWPGGMQAIVQYIHSKGLKAGIYIDAGPQGCGTRTDGSHFVGSDFAHYDHDFLQFAQWGY
CCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCC
DFVKVDFCGGSAAGYDPQQAYTAVASAIEKAYVQTGRRLTLSICDWGTIAGNPAYPDYKQ
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCC
GPWAWAAGVGRMWRTTGDIYVPNTGAPSYDRVLGNFFGNYHPESQHTGFYNDPDMMVAGM
CCCHHHHHCCHHEECCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
GMTAVQDQAHMSLWAIAGAPLILGNDLSKPLTADATNLMTNSGVIAVDQDPLGIQGVLVA
CCHHHCCCHHEEEEEECCCCEEECCCCCCCCCCCHHHHEECCCEEEECCCCCCEEEEEEE
QSGAQQVWAKLLAGSGQRAVVLFNNGTSDAPMTVTWQQLGFAPNSSATVRDLWAGKDLGS
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHCCCCCCCCEEEHHHCCCCCCC
FTTSYTAPTVPAGGAVMLAISGSDVASTVYEPTALGGGASYTRCSDCAGGKIVHGLGTVT
CCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCEE
FGNIAAANNSGGYVEITYANRTHETLTAQLTANGGQPTMVAFPPTGRDGKVGTVTLYVPF
ECCEEEECCCCCEEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEEEEEEEEE
TAGQNSISISSADSTAPAPEIATLAVVAGPVRLPPFHAAYEAEASNNVLAGGARTASCSA
ECCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCCEEECCCCCCCCCC
CSGGADVGYIGNGGTLTMNGITAAADGKYTVLIGYANGDSAPRSAEISFNGTTPVTVSFP
CCCCCCEEEECCCCEEEECCEEEECCCCEEEEEEEECCCCCCCEEEEEECCCCCEEEECC
PTGGWNTISTLAVTGTFKSGSANTLIFSNSSGWAPDIDGIGTPVAAN
CCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLALSGCGGDSTDGSGAASLMGAKNTAAAAGAQADAQRSSQPGAGSRQMAVVRPPMGWS
CEEEECCCCCCCCCCCCEEEECCCCCHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCC
SWNSLEENVNYNTIKAEADGLAALNAQIKSGAKYEYVNTDEGWWTSGVRDADGNFVIDNT
CHHHHHHCCCCEEEEECCCCEEEEEHHHCCCCEEEEEECCCCCEECCEEECCCCEEEECC
QWPGGMQAIVQYIHSKGLKAGIYIDAGPQGCGTRTDGSHFVGSDFAHYDHDFLQFAQWGY
CCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCC
DFVKVDFCGGSAAGYDPQQAYTAVASAIEKAYVQTGRRLTLSICDWGTIAGNPAYPDYKQ
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCC
GPWAWAAGVGRMWRTTGDIYVPNTGAPSYDRVLGNFFGNYHPESQHTGFYNDPDMMVAGM
CCCHHHHHCCHHEECCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
GMTAVQDQAHMSLWAIAGAPLILGNDLSKPLTADATNLMTNSGVIAVDQDPLGIQGVLVA
CCHHHCCCHHEEEEEECCCCEEECCCCCCCCCCCHHHHEECCCEEEECCCCCCEEEEEEE
QSGAQQVWAKLLAGSGQRAVVLFNNGTSDAPMTVTWQQLGFAPNSSATVRDLWAGKDLGS
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHCCCCCCCCEEEHHHCCCCCCC
FTTSYTAPTVPAGGAVMLAISGSDVASTVYEPTALGGGASYTRCSDCAGGKIVHGLGTVT
CCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCEE
FGNIAAANNSGGYVEITYANRTHETLTAQLTANGGQPTMVAFPPTGRDGKVGTVTLYVPF
ECCEEEECCCCCEEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEEEEEEEEE
TAGQNSISISSADSTAPAPEIATLAVVAGPVRLPPFHAAYEAEASNNVLAGGARTASCSA
ECCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCCEEECCCCCCCCCC
CSGGADVGYIGNGGTLTMNGITAAADGKYTVLIGYANGDSAPRSAEISFNGTTPVTVSFP
CCCCCCEEEECCCCEEEECCEEEECCCCEEEEEEEECCCCCCCEEEEEECCCCCEEEECC
PTGGWNTISTLAVTGTFKSGSANTLIFSNSSGWAPDIDGIGTPVAAN
CCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA