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The map label for this gene is pleD [H]

Identifier: 238023756

GI number: 238023756

Start: 335665

End: 336858

Strand: Direct

Name: pleD [H]

Synonym: bglu_2g02880

Alternate gene names: 238023756

Gene position: 335665-336858 (Clockwise)

Preceding gene: 238023755

Following gene: 238023757

Centisome position: 11.87

GC content: 71.86

Gene sequence:

>1194_bases
ATGTCCACCCCGACCGTGCTTATCGGCATAGCGGCCCTGTTCTGCCTGATGAGCGCCGCCGTCCTCGGCTCGCTGCTGCA
GGCCGGAATCCCGGGCGTGCGCCACTGGTGCATGGCAGTGGGCCTGCTGGGCGCTGCCGCGGTGCTGTTGCTGCTGCGCC
GCTCGGACAGCGCGGCGGGCGGCGCGAGCATGCTGCTGGTGACGGCGTCGGTGCTCGCCCTGCACGGCTTGCGGCGTTTC
TTCCTGCTGCGTGCCCCGGTGGTCTGGGAATCGGCCGCGTTCGGTCTGCTGATAGCCGGGCTCGCCTATTGGACCTGGCG
TTCGCCCGACCTCGACGCGCGCATCGTGCTGGTGTCGGGCGGCCTCGCCTACGTGCGCTTCGCGATCGCCTGGCTGGCGC
AGCGGCACCGGCCGGCGGGCCGGCCCAGGTACGTCTACGACTTCGTGTCGGTAATCGCGGCGCTCGGGGCGCTGGTCCAT
GCGGCCCGCTGCGCGGCCTATGGCTTCGGCTTCATGCATGACACGGTGTTCCTGGCGGCGACGCCGCTGAACATCGCGTT
TCTCGGCATGGCCACGCTGAGCTTGCCGTGCCTGTCGGTCGGCATGGTGATGCTGGCCCACGACCGCCTGATGGCGCGCA
TGGAGCGGCTGGCCACCATCGACGAGCTGACCGGCCTGCTGGGGCGGCGCGCCTTCATCGCGCGGGCCGAGGCGCTGCTC
GATTCGGCGCGGGCCGCGCAATCGCAGCTGTCCGTCGTGATTCTCGACATCGACGACTTCAAGCGCATCAACGACTGCTA
CGGGCACGCGGCCGGCGACCAGACGCTGGCGCACGTGGCCCTGACCATCGCGCGCGAGACGCGGCACGGCGACCTGCTGG
GCCGGATCGGCGGCGAGGAGTTCGCGCTGCTGATGCGCACCGGCAGGGAGGAAGCCGCGCGCCTGACGAACCGCTTGCGT
GCGCTCGTCGCCGCCTCGGCGGCGAGCGTGCCGGGCGGCAACCTCGCCTGCACGTTCAGCGCCGGCGTGGAGCAGATCGG
GGCCGGCGATACGCTGGCCTCGGCGATGGCGAGAGCCGATGGCGCGCTCTATGCGGCGAAGGCCAACGGCCGCAATTGCG
TGATGCTGGCGGAGGCGCCGCCGGTCTGGCAGGACCAGCCGAGCGGCGCGCGGATCTGGCAGCGGGCCGGATGA

Upstream 100 bases:

>100_bases
ATATCTTCCGGTACGATATGCGCTTTGCCTGCACCGGCAGGCGCACGATTCCGTGCCACGCCCGATGCCTTCATCGATCG
GACTTTCCCGTCACGCTGCC

Downstream 100 bases:

>100_bases
AATCGCGGACGCGAGGAGAGGCCAGGTATGGAGCAAACCCCCGAGGCGCTGGTCGCCGAACTCACGCAGCGCGGCGTGGC
GGTGTCGGCAGGGCACGTGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSTPTVLIGIAALFCLMSAAVLGSLLQAGIPGVRHWCMAVGLLGAAAVLLLLRRSDSAAGGASMLLVTASVLALHGLRRF
FLLRAPVVWESAAFGLLIAGLAYWTWRSPDLDARIVLVSGGLAYVRFAIAWLAQRHRPAGRPRYVYDFVSVIAALGALVH
AARCAAYGFGFMHDTVFLAATPLNIAFLGMATLSLPCLSVGMVMLAHDRLMARMERLATIDELTGLLGRRAFIARAEALL
DSARAAQSQLSVVILDIDDFKRINDCYGHAAGDQTLAHVALTIARETRHGDLLGRIGGEEFALLMRTGREEAARLTNRLR
ALVAASAASVPGGNLACTFSAGVEQIGAGDTLASAMARADGALYAAKANGRNCVMLAEAPPVWQDQPSGARIWQRAG

Sequences:

>Translated_397_residues
MSTPTVLIGIAALFCLMSAAVLGSLLQAGIPGVRHWCMAVGLLGAAAVLLLLRRSDSAAGGASMLLVTASVLALHGLRRF
FLLRAPVVWESAAFGLLIAGLAYWTWRSPDLDARIVLVSGGLAYVRFAIAWLAQRHRPAGRPRYVYDFVSVIAALGALVH
AARCAAYGFGFMHDTVFLAATPLNIAFLGMATLSLPCLSVGMVMLAHDRLMARMERLATIDELTGLLGRRAFIARAEALL
DSARAAQSQLSVVILDIDDFKRINDCYGHAAGDQTLAHVALTIARETRHGDLLGRIGGEEFALLMRTGREEAARLTNRLR
ALVAASAASVPGGNLACTFSAGVEQIGAGDTLASAMARADGALYAAKANGRNCVMLAEAPPVWQDQPSGARIWQRAG
>Mature_396_residues
STPTVLIGIAALFCLMSAAVLGSLLQAGIPGVRHWCMAVGLLGAAAVLLLLRRSDSAAGGASMLLVTASVLALHGLRRFF
LLRAPVVWESAAFGLLIAGLAYWTWRSPDLDARIVLVSGGLAYVRFAIAWLAQRHRPAGRPRYVYDFVSVIAALGALVHA
ARCAAYGFGFMHDTVFLAATPLNIAFLGMATLSLPCLSVGMVMLAHDRLMARMERLATIDELTGLLGRRAFIARAEALLD
SARAAQSQLSVVILDIDDFKRINDCYGHAAGDQTLAHVALTIARETRHGDLLGRIGGEEFALLMRTGREEAARLTNRLRA
LVAASAASVPGGNLACTFSAGVEQIGAGDTLASAMARADGALYAAKANGRNCVMLAEAPPVWQDQPSGARIWQRAG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=38.4615384615385, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787262, Length=191, Percent_Identity=34.5549738219895, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=36.5269461077844, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=33.9506172839506, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787802, Length=263, Percent_Identity=28.8973384030418, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=31.4814814814815, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=177, Percent_Identity=34.4632768361582, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=33.7278106508876, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=31.5508021390374, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=187, Percent_Identity=33.1550802139037, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=30.4878048780488, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081977, Length=166, Percent_Identity=30.1204819277108, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42001; Mature: 41869

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPTVLIGIAALFCLMSAAVLGSLLQAGIPGVRHWCMAVGLLGAAAVLLLLRRSDSAAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GASMLLVTASVLALHGLRRFFLLRAPVVWESAAFGLLIAGLAYWTWRSPDLDARIVLVSG
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
GLAYVRFAIAWLAQRHRPAGRPRYVYDFVSVIAALGALVHAARCAAYGFGFMHDTVFLAA
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEE
TPLNIAFLGMATLSLPCLSVGMVMLAHDRLMARMERLATIDELTGLLGRRAFIARAEALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSARAAQSQLSVVILDIDDFKRINDCYGHAAGDQTLAHVALTIARETRHGDLLGRIGGEE
HHHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHH
FALLMRTGREEAARLTNRLRALVAASAASVPGGNLACTFSAGVEQIGAGDTLASAMARAD
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCHHHHCCCHHHHHHHHHCC
GALYAAKANGRNCVMLAEAPPVWQDQPSGARIWQRAG
CCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
STPTVLIGIAALFCLMSAAVLGSLLQAGIPGVRHWCMAVGLLGAAAVLLLLRRSDSAAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GASMLLVTASVLALHGLRRFFLLRAPVVWESAAFGLLIAGLAYWTWRSPDLDARIVLVSG
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
GLAYVRFAIAWLAQRHRPAGRPRYVYDFVSVIAALGALVHAARCAAYGFGFMHDTVFLAA
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEE
TPLNIAFLGMATLSLPCLSVGMVMLAHDRLMARMERLATIDELTGLLGRRAFIARAEALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSARAAQSQLSVVILDIDDFKRINDCYGHAAGDQTLAHVALTIARETRHGDLLGRIGGEE
HHHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHH
FALLMRTGREEAARLTNRLRALVAASAASVPGGNLACTFSAGVEQIGAGDTLASAMARAD
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCHHHHCCCHHHHHHHHHCC
GALYAAKANGRNCVMLAEAPPVWQDQPSGARIWQRAG
CCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA