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The map label for this gene is mtnN

Identifier: 229918443

GI number: 229918443

Start: 2700135

End: 2700836

Strand: Direct

Name: mtnN

Synonym: EAT1b_2730

Alternate gene names: 229918443

Gene position: 2700135-2700836 (Clockwise)

Preceding gene: 229918442

Following gene: 229918444

Centisome position: 90.01

GC content: 51.0

Gene sequence:

>702_bases
ATGAAAGTAGGCATCATCGGCGCGATGGAAGAAGAAGTGAATTTACTTCGGAACGAGTTGACAGAACGAGTAGACACTGA
AATCGCGAACTATCATTTTTATGAGGGCTATCTTGGCAACATGCAAGTCGTCATCTTGAAGTCAGGCATCGGGAAGGTCA
ATGCAGCCATCGGCACGACACTTTTGATTGATAAGTTCAAACCGGACGTCGTCATCAACACAGGATCTGCCGGCGGTTTT
AAAAAGGGGATGAAAGTCGGAGACGTCGTCGTCTCGACGGAAGTTCGTCACCACGATGTCGACGTGACGGCATTCGGATA
TGAGTACGGACAAGTACCAGGCATGCCGCCGGCGTATGAGGCGGATGCCAAGCTCGTCAGCGTCTGTAAAGATGTCATTG
AAAACTTGCCGGACGTGAACGTGCACCAGGGATTGATTGTGACAGGAGATTCGTTCATCAACGATTCGAAACGCGTGGCG
GACATCCTCGGTCATTTCGATGGCGTCGCAGCCGTCGAGATGGAGGCTGCCCCGATCGCCCAAACGTGCTATCAGTTTGG
TGTCCCGTTCGTCGTCACACGCTCGATTTCAGACAGTGCGGACGAAGAAGCGAATCTGTCGTTTGATGAGTTCTTGGAGA
CAGCATCGATCAATTCTGCGAAGATGGTCATGGCTGTCACGAAGCGTTTAGAACAAGCTTAA

Upstream 100 bases:

>100_bases
AACACCAGGTGGCGACATGGTCGTCGAAATTAAAGAAATCAAATAAATTGTTGAGCGACCTCCTATCTGGAAGTCGCTCT
CTTTCTGAGGAGGAAACGGA

Downstream 100 bases:

>100_bases
TCCAGTCTACACGAGGAGGATGCATTGACATCCTCTTTTTATTCCCTTATATTAGTACCAGATACTAAGAATAGGGGTCG
ACGATATGAATATTGGAATC

Product: Adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKVGIIGAMEEEVNLLRNELTERVDTEIANYHFYEGYLGNMQVVILKSGIGKVNAAIGTTLLIDKFKPDVVINTGSAGGF
KKGMKVGDVVVSTEVRHHDVDVTAFGYEYGQVPGMPPAYEADAKLVSVCKDVIENLPDVNVHQGLIVTGDSFINDSKRVA
DILGHFDGVAAVEMEAAPIAQTCYQFGVPFVVTRSISDSADEEANLSFDEFLETASINSAKMVMAVTKRLEQA

Sequences:

>Translated_233_residues
MKVGIIGAMEEEVNLLRNELTERVDTEIANYHFYEGYLGNMQVVILKSGIGKVNAAIGTTLLIDKFKPDVVINTGSAGGF
KKGMKVGDVVVSTEVRHHDVDVTAFGYEYGQVPGMPPAYEADAKLVSVCKDVIENLPDVNVHQGLIVTGDSFINDSKRVA
DILGHFDGVAAVEMEAAPIAQTCYQFGVPFVVTRSISDSADEEANLSFDEFLETASINSAKMVMAVTKRLEQA
>Mature_233_residues
MKVGIIGAMEEEVNLLRNELTERVDTEIANYHFYEGYLGNMQVVILKSGIGKVNAAIGTTLLIDKFKPDVVINTGSAGGF
KKGMKVGDVVVSTEVRHHDVDVTAFGYEYGQVPGMPPAYEADAKLVSVCKDVIENLPDVNVHQGLIVTGDSFINDSKRVA
DILGHFDGVAAVEMEAAPIAQTCYQFGVPFVVTRSISDSADEEANLSFDEFLETASINSAKMVMAVTKRLEQA

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=230, Percent_Identity=53.0434782608696, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_EXISA (C4L559)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887089.1
- GeneID:   7868935
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2730
- OMA:   AMEQEVT
- ProtClustDB:   CLSK2489636
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 25194; Mature: 25194

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 153-153 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIIGAMEEEVNLLRNELTERVDTEIANYHFYEGYLGNMQVVILKSGIGKVNAAIGTT
CCEEEEECHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCEEEEEEECCCCHHHHHHHHE
LLIDKFKPDVVINTGSAGGFKKGMKVGDVVVSTEVRHHDVDVTAFGYEYGQVPGMPPAYE
EEEECCCCCEEEECCCCCCHHCCCEECCEEEECCHHHCCCEEEEECCCCCCCCCCCCCCC
ADAKLVSVCKDVIENLPDVNVHQGLIVTGDSFINDSKRVADILGHFDGVAAVEMEAAPIA
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHCCCEEEEEECCCHHH
QTCYQFGVPFVVTRSISDSADEEANLSFDEFLETASINSAKMVMAVTKRLEQA
HHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVGIIGAMEEEVNLLRNELTERVDTEIANYHFYEGYLGNMQVVILKSGIGKVNAAIGTT
CCEEEEECHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCEEEEEEECCCCHHHHHHHHE
LLIDKFKPDVVINTGSAGGFKKGMKVGDVVVSTEVRHHDVDVTAFGYEYGQVPGMPPAYE
EEEECCCCCEEEECCCCCCHHCCCEECCEEEECCHHHCCCEEEEECCCCCCCCCCCCCCC
ADAKLVSVCKDVIENLPDVNVHQGLIVTGDSFINDSKRVADILGHFDGVAAVEMEAAPIA
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHCCCEEEEEECCCHHH
QTCYQFGVPFVVTRSISDSADEEANLSFDEFLETASINSAKMVMAVTKRLEQA
HHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA