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The map label for this gene is greA [H]

Identifier: 229918442

GI number: 229918442

Start: 2699607

End: 2700080

Strand: Direct

Name: greA [H]

Synonym: EAT1b_2729

Alternate gene names: 229918442

Gene position: 2699607-2700080 (Clockwise)

Preceding gene: 229918441

Following gene: 229918443

Centisome position: 89.99

GC content: 46.41

Gene sequence:

>474_bases
ATGGCTGAAAAACAACACTATATGACGTTAGACGGAAAAGCGAAGATCGAACATGAATTAAACGAACTGAAGACGGTCCG
CCGTAAAGAAGTCGTTGAAAATATTAAGATTGCTCGTGACTTCGGTGACTTGTCAGAGAACGCGGAATATGACGCGGCGA
AAGAAGAACAAGCGATGGTCGAAGGACGCATCGCACAATTAGAAGAGATGCTCCGTAACGCGGTCATCATTCAAGATGAT
GCAGGCGATAACTCGGTTGTCTCAATCGGGAAGACGGTAACGTTCTTCATCCAAGAAGATAATGAAGACGAGACGTATAC
AATCGTTGGTGGAGCTGAGGCAGACCCGTTCACAGGGAAAATCTCGAACGAATCACCGATCGCCAAGGCACTTCTTGGTC
GTACGATTGGTGATGTGGTCACGGTGCAAACACCAGGTGGCGACATGGTCGTCGAAATTAAAGAAATCAAATAA

Upstream 100 bases:

>100_bases
CGTTGGGTGCTCACTGAGCGCAATCGCGCTTGAGGAAATAAATCGTTTTCAATTCAACCCTAGCTGTAATATACTAGGAG
ACAATGAAGGAGTGACAGAA

Downstream 100 bases:

>100_bases
ATTGTTGAGCGACCTCCTATCTGGAAGTCGCTCTCTTTCTGAGGAGGAAACGGAATGAAAGTAGGCATCATCGGCGCGAT
GGAAGAAGAAGTGAATTTAC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAEKQHYMTLDGKAKIEHELNELKTVRRKEVVENIKIARDFGDLSENAEYDAAKEEQAMVEGRIAQLEEMLRNAVIIQDD
AGDNSVVSIGKTVTFFIQEDNEDETYTIVGGAEADPFTGKISNESPIAKALLGRTIGDVVTVQTPGGDMVVEIKEIK

Sequences:

>Translated_157_residues
MAEKQHYMTLDGKAKIEHELNELKTVRRKEVVENIKIARDFGDLSENAEYDAAKEEQAMVEGRIAQLEEMLRNAVIIQDD
AGDNSVVSIGKTVTFFIQEDNEDETYTIVGGAEADPFTGKISNESPIAKALLGRTIGDVVTVQTPGGDMVVEIKEIK
>Mature_156_residues
AEKQHYMTLDGKAKIEHELNELKTVRRKEVVENIKIARDFGDLSENAEYDAAKEEQAMVEGRIAQLEEMLRNAVIIQDDA
GDNSVVSIGKTVTFFIQEDNEDETYTIVGGAEADPFTGKISNESPIAKALLGRTIGDVVTVQTPGGDMVVEIKEIK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=147, Percent_Identity=46.2585034013605, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI87082261, Length=144, Percent_Identity=31.9444444444444, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17328; Mature: 17196

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKQHYMTLDGKAKIEHELNELKTVRRKEVVENIKIARDFGDLSENAEYDAAKEEQAMV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
EGRIAQLEEMLRNAVIIQDDAGDNSVVSIGKTVTFFIQEDNEDETYTIVGGAEADPFTGK
HHHHHHHHHHHHCEEEEEECCCCCCEEEECCEEEEEEEECCCCCEEEEEECCCCCCCCCC
ISNESPIAKALLGRTIGDVVTVQTPGGDMVVEIKEIK
CCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEEEECC
>Mature Secondary Structure 
AEKQHYMTLDGKAKIEHELNELKTVRRKEVVENIKIARDFGDLSENAEYDAAKEEQAMV
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
EGRIAQLEEMLRNAVIIQDDAGDNSVVSIGKTVTFFIQEDNEDETYTIVGGAEADPFTGK
HHHHHHHHHHHHCEEEEEECCCCCCEEEECCEEEEEEEECCCCCEEEEEECCCCCCCCCC
ISNESPIAKALLGRTIGDVVTVQTPGGDMVVEIKEIK
CCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]