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The map label for this gene is ogt [H]

Identifier: 229918147

GI number: 229918147

Start: 2384193

End: 2384633

Strand: Direct

Name: ogt [H]

Synonym: EAT1b_2427

Alternate gene names: 229918147

Gene position: 2384193-2384633 (Clockwise)

Preceding gene: 229918146

Following gene: 229918148

Centisome position: 79.48

GC content: 49.89

Gene sequence:

>441_bases
ATGAAGATCGTGTCGTCTAAATGGGGACCGTTAGCGTATGAATTAAACGCAGCAAACAAACTGACGGCTCTTGAGTTCAC
AACACATGAGCCGACTGACGAGGCACCTGAGTGGTTGCAAGATCTCATGACGCGAGTTGAGCAGAATGGATTCCATGAAG
CAGATCGTGCCTACGTCGAAATGAACGGGACACCGTTTCAACAGGAGGTCTGGGATGCGCTCTTGACCATTCCTTATGGC
GAGACACGCACATATAAGCAAATTGCTGAACAAATCGGTCGGCCGAAAGCCGTTCGCGCCGTCGGTCAGGCGCTCAATCG
AAATCCGTTGCCGATTCTATTTCCGTGTCACCGTGTCATTGGAAGTAGCGGGAAGCTTGTCGGTTTTGCGGGAGGATTGG
CTGAGAAACAACGCCTTCTAGAATTTGAAATGAAAAAGTGA

Upstream 100 bases:

>100_bases
CTTGGACCTGAGGCCGATTCGATATTTGAAGAGATTGAACGGAATGAAACGTCTGACATGGTTCATGAAGAGATTCGTAT
CTATCGAGAGGAGTGGGCAC

Downstream 100 bases:

>100_bases
GGGAAATCAACCATGGCAATTCATGTCGTAATGTTTCAACCAGAAATACCAGCAAATACAGGGAATGTCTCACGTACGTG
TGCGGCGACGAACTCGGTGC

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKIVSSKWGPLAYELNAANKLTALEFTTHEPTDEAPEWLQDLMTRVEQNGFHEADRAYVEMNGTPFQQEVWDALLTIPYG
ETRTYKQIAEQIGRPKAVRAVGQALNRNPLPILFPCHRVIGSSGKLVGFAGGLAEKQRLLEFEMKK

Sequences:

>Translated_146_residues
MKIVSSKWGPLAYELNAANKLTALEFTTHEPTDEAPEWLQDLMTRVEQNGFHEADRAYVEMNGTPFQQEVWDALLTIPYG
ETRTYKQIAEQIGRPKAVRAVGQALNRNPLPILFPCHRVIGSSGKLVGFAGGLAEKQRLLEFEMKK
>Mature_146_residues
MKIVSSKWGPLAYELNAANKLTALEFTTHEPTDEAPEWLQDLMTRVEQNGFHEADRAYVEMNGTPFQQEVWDALLTIPYG
ETRTYKQIAEQIGRPKAVRAVGQALNRNPLPILFPCHRVIGSSGKLVGFAGGLAEKQRLLEFEMKK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=84, Percent_Identity=48.8095238095238, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787596, Length=82, Percent_Identity=56.0975609756098, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1788542, Length=85, Percent_Identity=51.7647058823529, Blast_Score=96, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=122, Percent_Identity=41.8032786885246, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=122, Percent_Identity=41.8032786885246, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=45.679012345679, Blast_Score=84, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17137554, Length=114, Percent_Identity=41.2280701754386, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16457; Mature: 16457

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSSKWGPLAYELNAANKLTALEFTTHEPTDEAPEWLQDLMTRVEQNGFHEADRAYVE
CCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
MNGTPFQQEVWDALLTIPYGETRTYKQIAEQIGRPKAVRAVGQALNRNPLPILFPCHRVI
ECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHHHH
GSSGKLVGFAGGLAEKQRLLEFEMKK
CCCCCEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIVSSKWGPLAYELNAANKLTALEFTTHEPTDEAPEWLQDLMTRVEQNGFHEADRAYVE
CCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
MNGTPFQQEVWDALLTIPYGETRTYKQIAEQIGRPKAVRAVGQALNRNPLPILFPCHRVI
ECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHHHH
GSSGKLVGFAGGLAEKQRLLEFEMKK
CCCCCEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]