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The map label for this gene is cspR [H]

Identifier: 229918148

GI number: 229918148

Start: 2384646

End: 2385119

Strand: Direct

Name: cspR [H]

Synonym: EAT1b_2428

Alternate gene names: 229918148

Gene position: 2384646-2385119 (Clockwise)

Preceding gene: 229918147

Following gene: 229918150

Centisome position: 79.49

GC content: 46.41

Gene sequence:

>474_bases
ATGGCAATTCATGTCGTAATGTTTCAACCAGAAATACCAGCAAATACAGGGAATGTCTCACGTACGTGTGCGGCGACGAA
CTCGGTGCTTCATTTGATTCGTCCGCTCGGGTTCTCGACGGATGATAAGATGTTAAAGCGTGCCGGTCTCGACTATTGGC
AATTTGTCGATGTACGCTATCATGACTCGTTAGAAGAGCTGTGGGAGAAGCATCCGGATGGTCAGTTCTTTTACATTACG
AAGTACGGGGAACAATACCCGAGTGATTTGGACGTTTCAGATGTAGACGGTGACTACTTCTTTGTCTTTGGTCGTGAGAC
GAAAGGGTTACCGATGGAAGTCATCGAGAACAATTTAGATCGCTGCATCCGTCTACCACAATCGAACCTCGTTCGTTCAT
TGAACGTATCGAATACAGCAGCCATCATCGTATATGAGGCGCTTCGCCAACAGGGATATGCAGGATTGAAATAA

Upstream 100 bases:

>100_bases
TGTCATTGGAAGTAGCGGGAAGCTTGTCGGTTTTGCGGGAGGATTGGCTGAGAAACAACGCCTTCTAGAATTTGAAATGA
AAAAGTGAGGGAAATCAACC

Downstream 100 bases:

>100_bases
AAAAGAGCCGGATTCGTAAGAATCCCGGCTCTTATTCGTTACCCTCAGGTTTACGGTTATATCCAGCTGTCAAAATCGCT
GCGAGGAAGACGACGCTGAT

Product: RNA methyltransferase, TrmH family, group 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAIHVVMFQPEIPANTGNVSRTCAATNSVLHLIRPLGFSTDDKMLKRAGLDYWQFVDVRYHDSLEELWEKHPDGQFFYIT
KYGEQYPSDLDVSDVDGDYFFVFGRETKGLPMEVIENNLDRCIRLPQSNLVRSLNVSNTAAIIVYEALRQQGYAGLK

Sequences:

>Translated_157_residues
MAIHVVMFQPEIPANTGNVSRTCAATNSVLHLIRPLGFSTDDKMLKRAGLDYWQFVDVRYHDSLEELWEKHPDGQFFYIT
KYGEQYPSDLDVSDVDGDYFFVFGRETKGLPMEVIENNLDRCIRLPQSNLVRSLNVSNTAAIIVYEALRQQGYAGLK
>Mature_156_residues
AIHVVMFQPEIPANTGNVSRTCAATNSVLHLIRPLGFSTDDKMLKRAGLDYWQFVDVRYHDSLEELWEKHPDGQFFYITK
YGEQYPSDLDVSDVDGDYFFVFGRETKGLPMEVIENNLDRCIRLPQSNLVRSLNVSNTAAIIVYEALRQQGYAGLK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=43.5064935064935, Blast_Score=128, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17954; Mature: 17822

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIHVVMFQPEIPANTGNVSRTCAATNSVLHLIRPLGFSTDDKMLKRAGLDYWQFVDVRY
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHEEEEEHH
HDSLEELWEKHPDGQFFYITKYGEQYPSDLDVSDVDGDYFFVFGRETKGLPMEVIENNLD
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCHH
RCIRLPQSNLVRSLNVSNTAAIIVYEALRQQGYAGLK
HHHHCCHHHHHHHCCCCCCHHHHEEHHHHHCCCCCCC
>Mature Secondary Structure 
AIHVVMFQPEIPANTGNVSRTCAATNSVLHLIRPLGFSTDDKMLKRAGLDYWQFVDVRY
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHEEEEEHH
HDSLEELWEKHPDGQFFYITKYGEQYPSDLDVSDVDGDYFFVFGRETKGLPMEVIENNLD
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCHH
RCIRLPQSNLVRSLNVSNTAAIIVYEALRQQGYAGLK
HHHHCCHHHHHHHCCCCCCHHHHEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]