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The map label for this gene is yfhQ [H]

Identifier: 229918134

GI number: 229918134

Start: 2365070

End: 2366104

Strand: Direct

Name: yfhQ [H]

Synonym: EAT1b_2414

Alternate gene names: 229918134

Gene position: 2365070-2366104 (Clockwise)

Preceding gene: 229918132

Following gene: 229918135

Centisome position: 78.84

GC content: 48.6

Gene sequence:

>1035_bases
ATGCTTAATCTTGATAAATTGTTCACAAATTATAATATTCACCATTTCAATGAGGAACTCGTCACCTGGTTTAATCGCGA
AAAGCGTGATTTACCTTGGCGTCATGCGAAAAATCCTTACCGTGTGTGGGTAAGTGAAGTCATGTTGCAACAGACACGAG
TCGATACGGTCATCCCATATTACAACCGGTTCATGGAACGCTTTCCGACGCTCGAAGATCTCGCAGCTGCTGACACAGAC
GAGGTCGTAAAGTATTGGGAAGGGCTAGGGTACTATTCTCGCGTCCGGAACTTACACGAAGCGGTCAAAGAAGTCGCTTC
TGTTTACGAGGGAATCGTCCCAGAGGAGAAAGAACGTTTCGAGAAACTAAAAGGGGTCGGACCGTATACGACGGGAGCCG
TACTGTCGATTGCGTACAATCAACCGGAACCGGCTGTGGATGGAAACGTCATGCGCGTCATGTCACGTCAATTCGGGATC
TATGACGACATCGCCATGCCGAAGACGCGCAAAATCTTTGAACAAGTCGTTCGTCGATTGATGGATCCGGCTCACGCATC
CGATTTCAACGAAGGCGTGATGGAGTTAGGTGCGACGGTCTGTACACCGAAAAATCCGATGTGCTCACTCTGTCCTGTTC
AAGACACATGTTATGCATATGCGCATCATGTACAAGATGAGCTGCCGGTCAAAACGAAGAAGGGAGCAGCACGTATCGAA
ATGTATGATGCGCTGTGCATCGAGAAAGATGGAAAAGTTGCCTACGAACAACGAGCGGATAAGGGGTTGCTTGCTGGGAT
GTGGCAATATCCGCTTATGGATCGAGGAACGGGGGAACAACTTAACGGAACGTATCTTGGGCAAGTGAAACATGTCTTCT
CTCATATCGTTTGGTATATCGATTTGTATCGCGTCGAGACACTTCCGACGGATGACGTCGTCTGGCTAGACGCTTCGGAG
CGTGAGGTGAAGACGGTTTCTGTGGCTCAACAAAAATTAGAACGATTGGCAGGTGGCGAACGTGAGACGATTTGA

Upstream 100 bases:

>100_bases
CGCAGTATCCAATGTGCGTCAGCACCTCCATCAATAATGAAAGAATTACGTCATCTATTCTTATTTATCATACAATATTT
TTAAAACGAGGGGAAACACA

Downstream 100 bases:

>100_bases
TGTAGAGTCGACATATGATACGACGGTCGATTCACTATGGGATTTGTTACAGGATGCCTCGCTTTTGACGAAACTGACGA
AGTTTCCTAAAGTACGCTTA

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLNLDKLFTNYNIHHFNEELVTWFNREKRDLPWRHAKNPYRVWVSEVMLQQTRVDTVIPYYNRFMERFPTLEDLAAADTD
EVVKYWEGLGYYSRVRNLHEAVKEVASVYEGIVPEEKERFEKLKGVGPYTTGAVLSIAYNQPEPAVDGNVMRVMSRQFGI
YDDIAMPKTRKIFEQVVRRLMDPAHASDFNEGVMELGATVCTPKNPMCSLCPVQDTCYAYAHHVQDELPVKTKKGAARIE
MYDALCIEKDGKVAYEQRADKGLLAGMWQYPLMDRGTGEQLNGTYLGQVKHVFSHIVWYIDLYRVETLPTDDVVWLDASE
REVKTVSVAQQKLERLAGGERETI

Sequences:

>Translated_344_residues
MLNLDKLFTNYNIHHFNEELVTWFNREKRDLPWRHAKNPYRVWVSEVMLQQTRVDTVIPYYNRFMERFPTLEDLAAADTD
EVVKYWEGLGYYSRVRNLHEAVKEVASVYEGIVPEEKERFEKLKGVGPYTTGAVLSIAYNQPEPAVDGNVMRVMSRQFGI
YDDIAMPKTRKIFEQVVRRLMDPAHASDFNEGVMELGATVCTPKNPMCSLCPVQDTCYAYAHHVQDELPVKTKKGAARIE
MYDALCIEKDGKVAYEQRADKGLLAGMWQYPLMDRGTGEQLNGTYLGQVKHVFSHIVWYIDLYRVETLPTDDVVWLDASE
REVKTVSVAQQKLERLAGGERETI
>Mature_344_residues
MLNLDKLFTNYNIHHFNEELVTWFNREKRDLPWRHAKNPYRVWVSEVMLQQTRVDTVIPYYNRFMERFPTLEDLAAADTD
EVVKYWEGLGYYSRVRNLHEAVKEVASVYEGIVPEEKERFEKLKGVGPYTTGAVLSIAYNQPEPAVDGNVMRVMSRQFGI
YDDIAMPKTRKIFEQVVRRLMDPAHASDFNEGVMELGATVCTPKNPMCSLCPVQDTCYAYAHHVQDELPVKTKKGAARIE
MYDALCIEKDGKVAYEQRADKGLLAGMWQYPLMDRGTGEQLNGTYLGQVKHVFSHIVWYIDLYRVETLPTDDVVWLDASE
REVKTVSVAQQKLERLAGGERETI

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8- oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal effici

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HhH domain [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=410, Percent_Identity=34.6341463414634, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI6912520, Length=410, Percent_Identity=34.6341463414634, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI115298650, Length=410, Percent_Identity=34.6341463414634, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI115298654, Length=410, Percent_Identity=34.6341463414634, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI115298652, Length=410, Percent_Identity=34.6341463414634, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI190358497, Length=410, Percent_Identity=34.6341463414634, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI1789331, Length=293, Percent_Identity=37.8839590443686, Blast_Score=210, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 39733; Mature: 39733

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLDKLFTNYNIHHFNEELVTWFNREKRDLPWRHAKNPYRVWVSEVMLQQTRVDTVIPY
CCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
YNRFMERFPTLEDLAAADTDEVVKYWEGLGYYSRVRNLHEAVKEVASVYEGIVPEEKERF
HHHHHHHCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EKLKGVGPYTTGAVLSIAYNQPEPAVDGNVMRVMSRQFGIYDDIAMPKTRKIFEQVVRRL
HHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MDPAHASDFNEGVMELGATVCTPKNPMCSLCPVQDTCYAYAHHVQDELPVKTKKGAARIE
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
MYDALCIEKDGKVAYEQRADKGLLAGMWQYPLMDRGTGEQLNGTYLGQVKHVFSHIVWYI
HHHHHHCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DLYRVETLPTDDVVWLDASEREVKTVSVAQQKLERLAGGERETI
HHHHEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLNLDKLFTNYNIHHFNEELVTWFNREKRDLPWRHAKNPYRVWVSEVMLQQTRVDTVIPY
CCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
YNRFMERFPTLEDLAAADTDEVVKYWEGLGYYSRVRNLHEAVKEVASVYEGIVPEEKERF
HHHHHHHCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EKLKGVGPYTTGAVLSIAYNQPEPAVDGNVMRVMSRQFGIYDDIAMPKTRKIFEQVVRRL
HHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MDPAHASDFNEGVMELGATVCTPKNPMCSLCPVQDTCYAYAHHVQDELPVKTKKGAARIE
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
MYDALCIEKDGKVAYEQRADKGLLAGMWQYPLMDRGTGEQLNGTYLGQVKHVFSHIVWYI
HHHHHHCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DLYRVETLPTDDVVWLDASEREVKTVSVAQQKLERLAGGERETI
HHHHEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]