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The map label for this gene is potC [H]

Identifier: 229918127

GI number: 229918127

Start: 2358585

End: 2359379

Strand: Direct

Name: potC [H]

Synonym: EAT1b_2407

Alternate gene names: 229918127

Gene position: 2358585-2359379 (Clockwise)

Preceding gene: 229918126

Following gene: 229918128

Centisome position: 78.62

GC content: 48.68

Gene sequence:

>795_bases
ATGAAACGTAAACGATTCAAATTATCAAATTTATACTTGGCGGCCGTCTTCTTCATCTTATATGCGCCAATCCTATATTT
GGTCTTCTACTCGTTCAACAGTGGCGGAACGATGAGTAACTTCGAAGGGTTCACCCTCGCATGGTACAAGTCGCTCTTCA
CGGATACGAGACTTCTCGTCATCGTCTTGAATACGATCGTCATCGCGCTGCTCTCGGCGACGATTTCGACCGTGCTCGGT
GTCATCGGGGCACTCGCCATCTATACGGCGAAACAAAAGTATACGAAGACGTCGCTTTTATCATTGAACAACGTCTTGAT
CGTGAGTCCGGACGTCATCATCGGGGCCTCCTTCTTGATCCTCTTCACGATGCTAGGGATTCAGTTAGGCTTTTATTCGG
TCCTCTTGTCGCACATCGCCTTCTCGGTTCCGATTGTCGTCATCTTGGTGTTGCCAAAACTCCAAGAGATGAGTCCGACG
TTGATTGATGCGTCACGTGACCTAGGCGCCAGTACGTGGGACGTATTGACGAAAGTCATTCTGCCATATATCACGCCAGG
GGTATTTGCCGGTTTCTTCACGGCACTTACCTATTCGTTAGATGACTTTGCTGTCACATTCTTCGTGACAGGAAACGGCT
TTACGACGCTCGCCGTAGAAGTCTATTCGCGGGCACGTCAAGGTATCTCGCTTGAAATCAATGCGTTGTCTGCGATTCTG
TTCCTCTTCACGCTCATCCTTGTCATCGGATATTATGTGTTGGCGCAACGAGGATCAAAGGAGGTTAGACGATGA

Upstream 100 bases:

>100_bases
ACTGGGGGATGGGCTCGACGATTGCCGTCTTCCTCATCTTGGCGATGGTTCTCGTCATGGTCGTCACACGTGACTGGGGT
GCGAAAGGAGCGGTTCGTCG

Downstream 100 bases:

>100_bases
AAAAACTAGTCATCATGTTGGCTGTACCATTGATTGTCAGCTTTGGTCTCCTTCTTTGGATTCAGCAACTGAATGCTGCG
CAAGGATTCGGCGGCGACAA

Product: binding-protein-dependent transport systems inner membrane component

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKRKRFKLSNLYLAAVFFILYAPILYLVFYSFNSGGTMSNFEGFTLAWYKSLFTDTRLLVIVLNTIVIALLSATISTVLG
VIGALAIYTAKQKYTKTSLLSLNNVLIVSPDVIIGASFLILFTMLGIQLGFYSVLLSHIAFSVPIVVILVLPKLQEMSPT
LIDASRDLGASTWDVLTKVILPYITPGVFAGFFTALTYSLDDFAVTFFVTGNGFTTLAVEVYSRARQGISLEINALSAIL
FLFTLILVIGYYVLAQRGSKEVRR

Sequences:

>Translated_264_residues
MKRKRFKLSNLYLAAVFFILYAPILYLVFYSFNSGGTMSNFEGFTLAWYKSLFTDTRLLVIVLNTIVIALLSATISTVLG
VIGALAIYTAKQKYTKTSLLSLNNVLIVSPDVIIGASFLILFTMLGIQLGFYSVLLSHIAFSVPIVVILVLPKLQEMSPT
LIDASRDLGASTWDVLTKVILPYITPGVFAGFFTALTYSLDDFAVTFFVTGNGFTTLAVEVYSRARQGISLEINALSAIL
FLFTLILVIGYYVLAQRGSKEVRR
>Mature_264_residues
MKRKRFKLSNLYLAAVFFILYAPILYLVFYSFNSGGTMSNFEGFTLAWYKSLFTDTRLLVIVLNTIVIALLSATISTVLG
VIGALAIYTAKQKYTKTSLLSLNNVLIVSPDVIIGASFLILFTMLGIQLGFYSVLLSHIAFSVPIVVILVLPKLQEMSPT
LIDASRDLGASTWDVLTKVILPYITPGVFAGFFTALTYSLDDFAVTFFVTGNGFTTLAVEVYSRARQGISLEINALSAIL
FLFTLILVIGYYVLAQRGSKEVRR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=230, Percent_Identity=38.695652173913, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1787081, Length=254, Percent_Identity=33.0708661417323, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787714, Length=244, Percent_Identity=26.6393442622951, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788764, Length=164, Percent_Identity=29.8780487804878, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29129; Mature: 29129

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKRFKLSNLYLAAVFFILYAPILYLVFYSFNSGGTMSNFEGFTLAWYKSLFTDTRLLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHH
IVLNTIVIALLSATISTVLGVIGALAIYTAKQKYTKTSLLSLNNVLIVSPDVIIGASFLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHH
LFTMLGIQLGFYSVLLSHIAFSVPIVVILVLPKLQEMSPTLIDASRDLGASTWDVLTKVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHH
LPYITPGVFAGFFTALTYSLDDFAVTFFVTGNGFTTLAVEVYSRARQGISLEINALSAIL
HHHHCHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHH
FLFTLILVIGYYVLAQRGSKEVRR
HHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MKRKRFKLSNLYLAAVFFILYAPILYLVFYSFNSGGTMSNFEGFTLAWYKSLFTDTRLLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHH
IVLNTIVIALLSATISTVLGVIGALAIYTAKQKYTKTSLLSLNNVLIVSPDVIIGASFLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHH
LFTMLGIQLGFYSVLLSHIAFSVPIVVILVLPKLQEMSPTLIDASRDLGASTWDVLTKVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHH
LPYITPGVFAGFFTALTYSLDDFAVTFFVTGNGFTTLAVEVYSRARQGISLEINALSAIL
HHHHCHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHH
FLFTLILVIGYYVLAQRGSKEVRR
HHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]