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The map label for this gene is potB [H]

Identifier: 229918126

GI number: 229918126

Start: 2357767

End: 2358588

Strand: Direct

Name: potB [H]

Synonym: EAT1b_2406

Alternate gene names: 229918126

Gene position: 2357767-2358588 (Clockwise)

Preceding gene: 229918125

Following gene: 229918127

Centisome position: 78.59

GC content: 46.35

Gene sequence:

>822_bases
ATGAATCAAACTAATCAGACCGCAAAGACGGTATATTTAACGACCTATTGGATTTGGATCGCCTTGTTTGTCATCGCACC
ACTCGTTCTCGTCGTCTATTATTCCTTTTTTGATATCGATGGGAATTTGAGTTTAGAAAACTATCAGACGTTCTTCACGT
CGACGTATTTGTCGATGACGCTCTCGTCGTTCTGGTATGCGTTCCTCATTACGTTCTTCTCGTTACTCGTCGGGTATCCG
ACGGCATACTTGTTGACGAAGACGAAGCACAAACAGCTTTGGCTCTTGCTCATCTTGCTGCCGTCATGGATTAACTTGTT
GTTAAAGGCGTATGCGTTCCTTGGTATTTTTGGAACGTACGGGATTACGAATCAATTTTTAGAAGCAATCGGTATTGGGA
CGAAACAGATTCTATTCACTGATTTCAGTTTCGTCTTCGTTTCGGTATACATCTTCATCCCGTTCATGATTTTGCCAATC
TACAACGCCATTGAGAAGCTACCGCCATCACTCGTTTACGCATCCCGTGATTTAGGTGCATCGAACTGGACGACGTTCCG
ACGTGTCATCTTCCCGTTGACGCTTGACGGTGTCAAATCGGGGATTCAAATTGTCTTTATTCCGGCATTATCACTCTTTA
TGATCACCCGACTCATCGCCGGGAACCGAGTCATCACACTCGGTACAGCGATTGAACAACAATTCCTTGTCACGCAAAAC
TGGGGGATGGGCTCGACGATTGCCGTCTTCCTCATCTTGGCGATGGTTCTCGTCATGGTCGTCACACGTGACTGGGGTGC
GAAAGGAGCGGTTCGTCGATGA

Upstream 100 bases:

>100_bases
ATCGGATTGACGTTTGACGAAGAAGCGATTCACGTCATGCGACTAGGTGAAACAGAAGAAGAATTTGACCGTCGTCTCGA
ATCCTACGAGGAGGTCCCTC

Downstream 100 bases:

>100_bases
AACGTAAACGATTCAAATTATCAAATTTATACTTGGCGGCCGTCTTCTTCATCTTATATGCGCCAATCCTATATTTGGTC
TTCTACTCGTTCAACAGTGG

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNQTNQTAKTVYLTTYWIWIALFVIAPLVLVVYYSFFDIDGNLSLENYQTFFTSTYLSMTLSSFWYAFLITFFSLLVGYP
TAYLLTKTKHKQLWLLLILLPSWINLLLKAYAFLGIFGTYGITNQFLEAIGIGTKQILFTDFSFVFVSVYIFIPFMILPI
YNAIEKLPPSLVYASRDLGASNWTTFRRVIFPLTLDGVKSGIQIVFIPALSLFMITRLIAGNRVITLGTAIEQQFLVTQN
WGMGSTIAVFLILAMVLVMVVTRDWGAKGAVRR

Sequences:

>Translated_273_residues
MNQTNQTAKTVYLTTYWIWIALFVIAPLVLVVYYSFFDIDGNLSLENYQTFFTSTYLSMTLSSFWYAFLITFFSLLVGYP
TAYLLTKTKHKQLWLLLILLPSWINLLLKAYAFLGIFGTYGITNQFLEAIGIGTKQILFTDFSFVFVSVYIFIPFMILPI
YNAIEKLPPSLVYASRDLGASNWTTFRRVIFPLTLDGVKSGIQIVFIPALSLFMITRLIAGNRVITLGTAIEQQFLVTQN
WGMGSTIAVFLILAMVLVMVVTRDWGAKGAVRR
>Mature_273_residues
MNQTNQTAKTVYLTTYWIWIALFVIAPLVLVVYYSFFDIDGNLSLENYQTFFTSTYLSMTLSSFWYAFLITFFSLLVGYP
TAYLLTKTKHKQLWLLLILLPSWINLLLKAYAFLGIFGTYGITNQFLEAIGIGTKQILFTDFSFVFVSVYIFIPFMILPI
YNAIEKLPPSLVYASRDLGASNWTTFRRVIFPLTLDGVKSGIQIVFIPALSLFMITRLIAGNRVITLGTAIEQQFLVTQN
WGMGSTIAVFLILAMVLVMVVTRDWGAKGAVRR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=253, Percent_Identity=35.1778656126482, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787080, Length=227, Percent_Identity=37.0044052863436, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787713, Length=255, Percent_Identity=27.843137254902, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31051; Mature: 31051

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTNQTAKTVYLTTYWIWIALFVIAPLVLVVYYSFFDIDGNLSLENYQTFFTSTYLSMT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LSSFWYAFLITFFSLLVGYPTAYLLTKTKHKQLWLLLILLPSWINLLLKAYAFLGIFGTY
HHHHHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GITNQFLEAIGIGTKQILFTDFSFVFVSVYIFIPFMILPIYNAIEKLPPSLVYASRDLGA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCC
SNWTTFRRVIFPLTLDGVKSGIQIVFIPALSLFMITRLIAGNRVITLGTAIEQQFLVTQN
CCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHEECC
WGMGSTIAVFLILAMVLVMVVTRDWGAKGAVRR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNQTNQTAKTVYLTTYWIWIALFVIAPLVLVVYYSFFDIDGNLSLENYQTFFTSTYLSMT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LSSFWYAFLITFFSLLVGYPTAYLLTKTKHKQLWLLLILLPSWINLLLKAYAFLGIFGTY
HHHHHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GITNQFLEAIGIGTKQILFTDFSFVFVSVYIFIPFMILPIYNAIEKLPPSLVYASRDLGA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCC
SNWTTFRRVIFPLTLDGVKSGIQIVFIPALSLFMITRLIAGNRVITLGTAIEQQFLVTQN
CCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHEECC
WGMGSTIAVFLILAMVLVMVVTRDWGAKGAVRR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]