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The map label for this gene is potD-A [H]

Identifier: 229918128

GI number: 229918128

Start: 2359376

End: 2360446

Strand: Direct

Name: potD-A [H]

Synonym: EAT1b_2408

Alternate gene names: 229918128

Gene position: 2359376-2360446 (Clockwise)

Preceding gene: 229918127

Following gene: 229918132

Centisome position: 78.65

GC content: 48.18

Gene sequence:

>1071_bases
ATGAAAAAACTAGTCATCATGTTGGCTGTACCATTGATTGTCAGCTTTGGTCTCCTTCTTTGGATTCAGCAACTGAATGC
TGCGCAAGGATTCGGCGGCGACAACACGCTTGTCGTTTACAACTGGGGAGATTATATCGATGAGGACTTGATCGGTGAGT
TCGAAGAGCAGAGCGGGTTGAAGGTCGTTTATCAAACGTTCGACTCGAACGAAGCGATGCTCACGAAAATTGAGCAAGGC
GGTACGGCCTTTGATGTCGCCGTTCCATCCGATTATGCGATCAGTAAGATGAGAGAGGAGAATCTCCTTCTCCCAATCGA
TTACGATAAACTCGAAAACTTTGATAATATCGATCCGCGCTTCCTCGATAAATCGTTCGACCCGGGTAACAAGTACTCGG
TTCCTTATTTCTGGGGTACGGTCGGGATCGTCTACAATCCGGAACTAGTCGATGGTGAGATCACTTCATGGAACGACCTG
TGGGAAATGCCGCTCGAGAACGACATTCTCCTTGCGGACGGGACACGTGAAGTGATGGGCTTCGGCCTGAACTCGCTTGG
GTACTCCCTCAACACGACGAATAAAGATGAGTTGATTGAGGCGGAAGAAAAGTTAACGACGCTTTGGCCGAACATTAAAG
CCATCGTCGGAGACGAAATCAAAATGCTGATGGCGAACCGTGAGGCAGCGGCAGCCGTCGTCTGGTCCGGGGATGCGTCA
GAAATCATGTATGAGAACGAAGAACTGACGTATGTCATTCCGGTAGAAGGCACGAATCTCTGGTTTGATAACCTCGTCAT
CCCAAGTACGGCACAAAACGTGGACGGGGCACATCAGTTCATCGACTTTATGTTAGATGCTGAGATTGCGGCACGTAACA
CGGATTATGTCGGATACTCAACACCGAACGAAGCGGCTCTCGAATTCTTGGATGAGGAAGTCACATCGGATGAACGATTC
TACCCGTCTCGTGAAGTGACGCAGAACCTTGAAGTGTATGAAAACCTAGGGAAGCGCATGAACGCTTACTATAATGAATT
GTATTTACGCTTTAAAATGCAGAGTAAATGA

Upstream 100 bases:

>100_bases
TTGAAATCAATGCGTTGTCTGCGATTCTGTTCCTCTTCACGCTCATCCTTGTCATCGGATATTATGTGTTGGCGCAACGA
GGATCAAAGGAGGTTAGACG

Downstream 100 bases:

>100_bases
CGAAACATCCCCGCGCTCATCATGAGTACGGGGATGTTTGTTTACTGCTTGATTGTCGATTTGATGTCTTCGATATGTTT
CAAGGTGACAGTCGTGTCAA

Product: extracellular solute-binding protein family 1

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKKLVIMLAVPLIVSFGLLLWIQQLNAAQGFGGDNTLVVYNWGDYIDEDLIGEFEEQSGLKVVYQTFDSNEAMLTKIEQG
GTAFDVAVPSDYAISKMREENLLLPIDYDKLENFDNIDPRFLDKSFDPGNKYSVPYFWGTVGIVYNPELVDGEITSWNDL
WEMPLENDILLADGTREVMGFGLNSLGYSLNTTNKDELIEAEEKLTTLWPNIKAIVGDEIKMLMANREAAAAVVWSGDAS
EIMYENEELTYVIPVEGTNLWFDNLVIPSTAQNVDGAHQFIDFMLDAEIAARNTDYVGYSTPNEAALEFLDEEVTSDERF
YPSREVTQNLEVYENLGKRMNAYYNELYLRFKMQSK

Sequences:

>Translated_356_residues
MKKLVIMLAVPLIVSFGLLLWIQQLNAAQGFGGDNTLVVYNWGDYIDEDLIGEFEEQSGLKVVYQTFDSNEAMLTKIEQG
GTAFDVAVPSDYAISKMREENLLLPIDYDKLENFDNIDPRFLDKSFDPGNKYSVPYFWGTVGIVYNPELVDGEITSWNDL
WEMPLENDILLADGTREVMGFGLNSLGYSLNTTNKDELIEAEEKLTTLWPNIKAIVGDEIKMLMANREAAAAVVWSGDAS
EIMYENEELTYVIPVEGTNLWFDNLVIPSTAQNVDGAHQFIDFMLDAEIAARNTDYVGYSTPNEAALEFLDEEVTSDERF
YPSREVTQNLEVYENLGKRMNAYYNELYLRFKMQSK
>Mature_356_residues
MKKLVIMLAVPLIVSFGLLLWIQQLNAAQGFGGDNTLVVYNWGDYIDEDLIGEFEEQSGLKVVYQTFDSNEAMLTKIEQG
GTAFDVAVPSDYAISKMREENLLLPIDYDKLENFDNIDPRFLDKSFDPGNKYSVPYFWGTVGIVYNPELVDGEITSWNDL
WEMPLENDILLADGTREVMGFGLNSLGYSLNTTNKDELIEAEEKLTTLWPNIKAIVGDEIKMLMANREAAAAVVWSGDAS
EIMYENEELTYVIPVEGTNLWFDNLVIPSTAQNVDGAHQFIDFMLDAEIAARNTDYVGYSTPNEAALEFLDEEVTSDERF
YPSREVTQNLEVYENLGKRMNAYYNELYLRFKMQSK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=38.6292834890966, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1787078, Length=315, Percent_Identity=32.6984126984127, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40450; Mature: 40450

Theoretical pI: Translated: 3.85; Mature: 3.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVIMLAVPLIVSFGLLLWIQQLNAAQGFGGDNTLVVYNWGDYIDEDLIGEFEEQSGL
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCCHHHCCCC
KVVYQTFDSNEAMLTKIEQGGTAFDVAVPSDYAISKMREENLLLPIDYDKLENFDNIDPR
EEEEEECCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHCCCEEEECHHHHCCCCCCCHH
FLDKSFDPGNKYSVPYFWGTVGIVYNPELVDGEITSWNDLWEMPLENDILLADGTREVMG
HHCCCCCCCCCEECCEEEEEEEEEECCEEECCCCCCCHHHHCCCCCCCEEEECCCHHHHH
FGLNSLGYSLNTTNKDELIEAEEKLTTLWPNIKAIVGDEIKMLMANREAAAAVVWSGDAS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCH
EIMYENEELTYVIPVEGTNLWFDNLVIPSTAQNVDGAHQFIDFMLDAEIAARNTDYVGYS
HEEECCCCEEEEEEECCCCEEECCEECCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCC
TPNEAALEFLDEEVTSDERFYPSREVTQNLEVYENLGKRMNAYYNELYLRFKMQSK
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCC
>Mature Secondary Structure
MKKLVIMLAVPLIVSFGLLLWIQQLNAAQGFGGDNTLVVYNWGDYIDEDLIGEFEEQSGL
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCCHHHCCCC
KVVYQTFDSNEAMLTKIEQGGTAFDVAVPSDYAISKMREENLLLPIDYDKLENFDNIDPR
EEEEEECCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHCCCEEEECHHHHCCCCCCCHH
FLDKSFDPGNKYSVPYFWGTVGIVYNPELVDGEITSWNDLWEMPLENDILLADGTREVMG
HHCCCCCCCCCEECCEEEEEEEEEECCEEECCCCCCCHHHHCCCCCCCEEEECCCHHHHH
FGLNSLGYSLNTTNKDELIEAEEKLTTLWPNIKAIVGDEIKMLMANREAAAAVVWSGDAS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCH
EIMYENEELTYVIPVEGTNLWFDNLVIPSTAQNVDGAHQFIDFMLDAEIAARNTDYVGYS
HEEECCCCEEEEEEECCCCEEECCEECCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCC
TPNEAALEFLDEEVTSDERFYPSREVTQNLEVYENLGKRMNAYYNELYLRFKMQSK
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]