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The map label for this gene is mtlF [H]

Identifier: 229917924

GI number: 229917924

Start: 2137464

End: 2137895

Strand: Direct

Name: mtlF [H]

Synonym: EAT1b_2203

Alternate gene names: 229917924

Gene position: 2137464-2137895 (Clockwise)

Preceding gene: 229917923

Following gene: 229917925

Centisome position: 71.25

GC content: 50.69

Gene sequence:

>432_bases
ATGCCATTCATTCAAACAGAGATGATTCGACTTAACCAAACATTCGCAACGACAGAGGAAGCGATCACAGCAGCCGGAGC
CGTCCTACAAGACGCCGGCTGTGTGACGCCGGAGTATGTAGAAGCGATGCATGAACGTCAAGCGATCGCCAACGTCTATA
TCGGAAATTACGTCGCCATTCCACACGGTACAGAAGCAGCGAAATCAGCGGTTCAGAAGACGGGGATCTCGTTCCTGCAA
GTACCAGACGGGGTCGACTTTGGGGGTCAAACGGCCAAACTCGTCATCGGAATTGCAGGCGTGAATGATGAACATTTAGA
GCTCCTATCGCAAATCGCGATTCTATGCTCAGACGAAAGCAATGTGGAGCGTCTTGTTCAGGCGAAAACGAAGGAAGAAA
TCTTGGCGCTCTTTATGGAAGAGGTGGCGTAA

Upstream 100 bases:

>100_bases
ACTACGACCAATTGGTAGAAGAAATTAAAGCGGAACGCAGCAAAATTTCTTGAACTCAGAACAGATAGAGATACGGTTTA
GCCATTAGGAGGAAATCAAA

Downstream 100 bases:

>100_bases
ATGAAAGCTGTTCACTTTGGGGCAGGGAACATCGGACGAGGCTTTATCGGATTATTGCTCAACCAAAGCGGTTACGAGGT
GACGTTTGTCGACATCAATG

Product: Protein-N(pi)-phosphohistidine--sugar phosphotransferase

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MPFIQTEMIRLNQTFATTEEAITAAGAVLQDAGCVTPEYVEAMHERQAIANVYIGNYVAIPHGTEAAKSAVQKTGISFLQ
VPDGVDFGGQTAKLVIGIAGVNDEHLELLSQIAILCSDESNVERLVQAKTKEEILALFMEEVA

Sequences:

>Translated_143_residues
MPFIQTEMIRLNQTFATTEEAITAAGAVLQDAGCVTPEYVEAMHERQAIANVYIGNYVAIPHGTEAAKSAVQKTGISFLQ
VPDGVDFGGQTAKLVIGIAGVNDEHLELLSQIAILCSDESNVERLVQAKTKEEILALFMEEVA
>Mature_142_residues
PFIQTEMIRLNQTFATTEEAITAAGAVLQDAGCVTPEYVEAMHERQAIANVYIGNYVAIPHGTEAAKSAVQKTGISFLQV
PDGVDFGGQTAKLVIGIAGVNDEHLELLSQIAILCSDESNVERLVQAKTKEEILALFMEEVA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=135, Percent_Identity=44.4444444444444, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1788494, Length=124, Percent_Identity=45.9677419354839, Blast_Score=100, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15379; Mature: 15247

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFIQTEMIRLNQTFATTEEAITAAGAVLQDAGCVTPEYVEAMHERQAIANVYIGNYVAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEC
PHGTEAAKSAVQKTGISFLQVPDGVDFGGQTAKLVIGIAGVNDEHLELLSQIAILCSDES
CCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCH
NVERLVQAKTKEEILALFMEEVA
HHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PFIQTEMIRLNQTFATTEEAITAAGAVLQDAGCVTPEYVEAMHERQAIANVYIGNYVAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEC
PHGTEAAKSAVQKTGISFLQVPDGVDFGGQTAKLVIGIAGVNDEHLELLSQIAILCSDES
CCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCH
NVERLVQAKTKEEILALFMEEVA
HHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]