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The map label for this gene is mtlA [H]

Identifier: 229917923

GI number: 229917923

Start: 2135995

End: 2137416

Strand: Direct

Name: mtlA [H]

Synonym: EAT1b_2202

Alternate gene names: 229917923

Gene position: 2135995-2137416 (Clockwise)

Preceding gene: 229917922

Following gene: 229917924

Centisome position: 71.2

GC content: 50.98

Gene sequence:

>1422_bases
ATGGCGAATACGCAAGCGAGCTCGGGCGGGGTCCGAGTCAAAGTTCAACGATTCGGTAGTTTCCTCAGTGGGATGGTCAT
GCCAAACATCGGTGCCTTCATTGCCTGGGGGATTATCACAGCACTCTTCATTCCGACAGGATGGTGGCCAAACGAAAACT
TAGCAGAACTCGTTGGTCCAACGATCACATATCTCTTACCATTACTCATCGGTTTCACTGGTGGTAAATTACTTCACGAC
GTACGAGGCGGTGTCGTCGGGGCACTCGCCACGATGGGGGTCATCGTCGGAACAGATATTCCGATGTTCCTCGGTGCGAT
GATCATGGGTCCACTCGGTGGTTATGTCATCCGCCAAGTCGATCGTGCCCTTGAAGGCAAAGTTAAAGCAGGATTTGAAA
TGCTCGTCAACAACTTCTCAGTGGGAATCATCGGTGGACTTCTCATGCTTGGAGGATTTGAAATCTTTGGTCCGATCATG
AGTGCCCTCGATAAAGTGATGGCGGCAGGTGTTGACTGGATCGTCGGCGCGAAATTGCTTCCGGTCGCAAGTCTGTTCAT
CGAACCGGCAAAAGTTCTCTTCTTGAACAACGCCATCAACCACGGGATCTTGAGTCCGCTTGGTGTCGATCAAGTCGCAG
ACGCTGGAAAGTCCGTTCTCTTCTTACTTGAAGCAAACCCAGGACCAGGTCTTGGATTACTTCTCGCCTATATGTTCTTC
GCCAAAGGTGCAGCGAAACGTACGGCACCGGGTGCAGCCATCATTCACTTCCTTGGTGGGATTCACGAGATTTACTTCCC
GTACGTATTGATGAAGCCGGTCTTGATCCTTGCGATGATCGCAGGGGGCGCAACAGGGATCTTCACGTTCGTACTCTTTG
ATGTCGGTCTCGTCGCACCTGCTTCACCAGGTAGTATCTTTGCCATTCTTGCGATGGCATCACGCGGTTCATATCTTGGA
CTCGTGGCAGGGATTCTCTTGTCAGCAGCGGTCACGTTCGCTGTGGCGAGTGTCATCTTGAAATCGAGCGCAGCAGAGGA
AGTATCACTCGAAGAAGCGACACAAAACGTGAGTGCGATGAAAGGCAAACAGTCTTACGCATCGGCTCTCGTGACAGATC
GTCTTGATAAAGTCGACCACATCATCTTCGCTTGTGACGCTGGAATGGGATCGAGTGCCATGGGAGCATCAGTTCTCCGG
AACAAGGTCAACAAAGCGAATTTGCCGATCAAAGTGACGAACACGTCCATCAGCAACTTGCCGAAAGATGCTCAGTTCGT
CATCACACACGAAGATTTGACGGAGCGTGCGGAAGCGCAAGCACCGAATGCGATTCATCGTTCGGTCACGAACTTCTTGA
ACGCCGATTACTACGACCAATTGGTAGAAGAAATTAAAGCGGAACGCAGCAAAATTTCTTGA

Upstream 100 bases:

>100_bases
AAGCGCTTACTCCGAGCATACAATATGGGTGTAGAGCACAAACCGGTTAGCTACACTACACATTCAACCTTTCACATTTA
AACGACTGGAGGTTTTATAC

Downstream 100 bases:

>100_bases
ACTCAGAACAGATAGAGATACGGTTTAGCCATTAGGAGGAAATCAAAATGCCATTCATTCAAACAGAGATGATTCGACTT
AACCAAACATTCGCAACGAC

Product: PTS system, mannitol-specific IIC subunit

Products: NA

Alternate protein names: EIICB-Mtl; EII-Mtl; Mannitol permease IIC component; PTS system mannitol-specific EIIC component; Mannitol-specific phosphotransferase enzyme IIB component; PTS system mannitol-specific EIIB component [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MANTQASSGGVRVKVQRFGSFLSGMVMPNIGAFIAWGIITALFIPTGWWPNENLAELVGPTITYLLPLLIGFTGGKLLHD
VRGGVVGALATMGVIVGTDIPMFLGAMIMGPLGGYVIRQVDRALEGKVKAGFEMLVNNFSVGIIGGLLMLGGFEIFGPIM
SALDKVMAAGVDWIVGAKLLPVASLFIEPAKVLFLNNAINHGILSPLGVDQVADAGKSVLFLLEANPGPGLGLLLAYMFF
AKGAAKRTAPGAAIIHFLGGIHEIYFPYVLMKPVLILAMIAGGATGIFTFVLFDVGLVAPASPGSIFAILAMASRGSYLG
LVAGILLSAAVTFAVASVILKSSAAEEVSLEEATQNVSAMKGKQSYASALVTDRLDKVDHIIFACDAGMGSSAMGASVLR
NKVNKANLPIKVTNTSISNLPKDAQFVITHEDLTERAEAQAPNAIHRSVTNFLNADYYDQLVEEIKAERSKIS

Sequences:

>Translated_473_residues
MANTQASSGGVRVKVQRFGSFLSGMVMPNIGAFIAWGIITALFIPTGWWPNENLAELVGPTITYLLPLLIGFTGGKLLHD
VRGGVVGALATMGVIVGTDIPMFLGAMIMGPLGGYVIRQVDRALEGKVKAGFEMLVNNFSVGIIGGLLMLGGFEIFGPIM
SALDKVMAAGVDWIVGAKLLPVASLFIEPAKVLFLNNAINHGILSPLGVDQVADAGKSVLFLLEANPGPGLGLLLAYMFF
AKGAAKRTAPGAAIIHFLGGIHEIYFPYVLMKPVLILAMIAGGATGIFTFVLFDVGLVAPASPGSIFAILAMASRGSYLG
LVAGILLSAAVTFAVASVILKSSAAEEVSLEEATQNVSAMKGKQSYASALVTDRLDKVDHIIFACDAGMGSSAMGASVLR
NKVNKANLPIKVTNTSISNLPKDAQFVITHEDLTERAEAQAPNAIHRSVTNFLNADYYDQLVEEIKAERSKIS
>Mature_472_residues
ANTQASSGGVRVKVQRFGSFLSGMVMPNIGAFIAWGIITALFIPTGWWPNENLAELVGPTITYLLPLLIGFTGGKLLHDV
RGGVVGALATMGVIVGTDIPMFLGAMIMGPLGGYVIRQVDRALEGKVKAGFEMLVNNFSVGIIGGLLMLGGFEIFGPIMS
ALDKVMAAGVDWIVGAKLLPVASLFIEPAKVLFLNNAINHGILSPLGVDQVADAGKSVLFLLEANPGPGLGLLLAYMFFA
KGAAKRTAPGAAIIHFLGGIHEIYFPYVLMKPVLILAMIAGGATGIFTFVLFDVGLVAPASPGSIFAILAMASRGSYLGL
VAGILLSAAVTFAVASVILKSSAAEEVSLEEATQNVSAMKGKQSYASALVTDRLDKVDHIIFACDAGMGSSAMGASVLRN
KVNKANLPIKVTNTSISNLPKDAQFVITHEDLTERAEAQAPNAIHRSVTNFLNADYYDQLVEEIKAERSKIS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2213

COG function: function code G; Phosphotransferase system, mannitol-specific IIBC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=468, Percent_Identity=62.1794871794872, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1789301, Length=461, Percent_Identity=53.5791757049892, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004718 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49609; Mature: 49478

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTQASSGGVRVKVQRFGSFLSGMVMPNIGAFIAWGIITALFIPTGWWPNENLAELVGP
CCCCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCH
TITYLLPLLIGFTGGKLLHDVRGGVVGALATMGVIVGTDIPMFLGAMIMGPLGGYVIRQV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DRALEGKVKAGFEMLVNNFSVGIIGGLLMLGGFEIFGPIMSALDKVMAAGVDWIVGAKLL
HHHHHCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVASLFIEPAKVLFLNNAINHGILSPLGVDQVADAGKSVLFLLEANPGPGLGLLLAYMFF
HHHHHHHCHHHHHEEHHHHCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
AKGAAKRTAPGAAIIHFLGGIHEIYFPYVLMKPVLILAMIAGGATGIFTFVLFDVGLVAP
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
ASPGSIFAILAMASRGSYLGLVAGILLSAAVTFAVASVILKSSAAEEVSLEEATQNVSAM
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KGKQSYASALVTDRLDKVDHIIFACDAGMGSSAMGASVLRNKVNKANLPIKVTNTSISNL
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHCC
PKDAQFVITHEDLTERAEAQAPNAIHRSVTNFLNADYYDQLVEEIKAERSKIS
CCCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANTQASSGGVRVKVQRFGSFLSGMVMPNIGAFIAWGIITALFIPTGWWPNENLAELVGP
CCCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCH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CCCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8824601 [H]