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The map label for this gene is fdnH [H]

Identifier: 229917628

GI number: 229917628

Start: 1869301

End: 1869843

Strand: Direct

Name: fdnH [H]

Synonym: EAT1b_1904

Alternate gene names: 229917628

Gene position: 1869301-1869843 (Clockwise)

Preceding gene: 229917627

Following gene: 229917629

Centisome position: 62.31

GC content: 49.54

Gene sequence:

>543_bases
ATGAATAAGGTCATGTATTTAGAGTTTGAACGTTGTATCGGCTGTCGGGCCTGTCAGGCAGCTTGCCGCGAATGCGGGGA
CCACGAGGGGCTCGAGCGCAACTATGTGGATTATATCGATTTCTCGGAGAACCGTCAGACGTTCCCGATGATCTGTATGC
AGTGTAAAAATCCGGCTTGTGCTCAAGTATGTCCGGCGAATGCGATTCAAATCACGCCAGAGGGCGTCGTGTTATCGGCT
TCAGAAGAAAAATGTATCGGCTGCCGGAACTGTACGTTCGCTTGTCCGTTCGGGATTCCGAAGTTCGACTTTGAGAAGAA
TAAGATGTATAAGTGCGACATGTGTTACGACCGCTCCAAACATGATATCGCGCCGATGTGTGCGTCGGTTTGTCCGTCGG
ATGCGATTCGCTTCATCGACCATGACGAGATGATGCAACTCCGTCGTCAACGCGTCCAAATGAACATGATCGAAGGGAAA
AAGCCTTCGCAAGACGATAAATGGAATTACGTCCCTGAATTCTTTGGCGTCTATTCCGATTGA

Upstream 100 bases:

>100_bases
TTAATCCGTTGACGGTCCCGCTCGGATCAAGCGTGCCGTTCATGGCGGACCATGCGATTGCGACGAGCTGTCACGTCATG
GATGAGGAGGTTCATACGAA

Downstream 100 bases:

>100_bases
GATGGAAAGGAAGATGACTTATGTCGAATGAATCAAAAGACCGGGAACGCTTATCCGAACTCGTCGATAACTTGAGTCGG
GCCGATGACGTGAAACTGAA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MNKVMYLEFERCIGCRACQAACRECGDHEGLERNYVDYIDFSENRQTFPMICMQCKNPACAQVCPANAIQITPEGVVLSA
SEEKCIGCRNCTFACPFGIPKFDFEKNKMYKCDMCYDRSKHDIAPMCASVCPSDAIRFIDHDEMMQLRRQRVQMNMIEGK
KPSQDDKWNYVPEFFGVYSD

Sequences:

>Translated_180_residues
MNKVMYLEFERCIGCRACQAACRECGDHEGLERNYVDYIDFSENRQTFPMICMQCKNPACAQVCPANAIQITPEGVVLSA
SEEKCIGCRNCTFACPFGIPKFDFEKNKMYKCDMCYDRSKHDIAPMCASVCPSDAIRFIDHDEMMQLRRQRVQMNMIEGK
KPSQDDKWNYVPEFFGVYSD
>Mature_180_residues
MNKVMYLEFERCIGCRACQAACRECGDHEGLERNYVDYIDFSENRQTFPMICMQCKNPACAQVCPANAIQITPEGVVLSA
SEEKCIGCRNCTFACPFGIPKFDFEKNKMYKCDMCYDRSKHDIAPMCASVCPSDAIRFIDHDEMMQLRRQRVQMNMIEGK
KPSQDDKWNYVPEFFGVYSD

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=167, Percent_Identity=34.7305389221557, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1790326, Length=191, Percent_Identity=32.4607329842932, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082180, Length=167, Percent_Identity=34.1317365269461, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787122, Length=166, Percent_Identity=31.9277108433735, Blast_Score=94, Evalue=4e-21,
Organism=Escherichia coli, GI1787872, Length=155, Percent_Identity=34.1935483870968, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI87082179, Length=163, Percent_Identity=29.4478527607362, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789370, Length=184, Percent_Identity=26.6304347826087, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI2367245, Length=163, Percent_Identity=31.2883435582822, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1789079, Length=189, Percent_Identity=26.4550264550265, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788811, Length=164, Percent_Identity=27.4390243902439, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1789067, Length=178, Percent_Identity=28.0898876404494, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI87082114, Length=191, Percent_Identity=26.1780104712042, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1787963, Length=160, Percent_Identity=28.125, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI2367345, Length=184, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI226510944, Length=147, Percent_Identity=29.2517006802721, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 20779; Mature: 20779

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.4 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
15.6 %Cys+Met (Translated Protein)
9.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
15.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVMYLEFERCIGCRACQAACRECGDHEGLERNYVDYIDFSENRQTFPMICMQCKNPAC
CCCEEEEEHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCH
AQVCPANAIQITPEGVVLSASEEKCIGCRNCTFACPFGIPKFDFEKNKMYKCDMCYDRSK
HHHCCCCEEEECCCCEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEECCCCCCCC
HDIAPMCASVCPSDAIRFIDHDEMMQLRRQRVQMNMIEGKKPSQDDKWNYVPEFFGVYSD
CCHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MNKVMYLEFERCIGCRACQAACRECGDHEGLERNYVDYIDFSENRQTFPMICMQCKNPAC
CCCEEEEEHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCH
AQVCPANAIQITPEGVVLSASEEKCIGCRNCTFACPFGIPKFDFEKNKMYKCDMCYDRSK
HHHCCCCEEEECCCCEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEECCCCCCCC
HDIAPMCASVCPSDAIRFIDHDEMMQLRRQRVQMNMIEGKKPSQDDKWNYVPEFFGVYSD
CCHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]