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The map label for this gene is nasC [H]

Identifier: 229917627

GI number: 229917627

Start: 1867058

End: 1869304

Strand: Direct

Name: nasC [H]

Synonym: EAT1b_1903

Alternate gene names: 229917627

Gene position: 1867058-1869304 (Clockwise)

Preceding gene: 229917626

Following gene: 229917628

Centisome position: 62.24

GC content: 53.18

Gene sequence:

>2247_bases
ATGAGTGACTTTTTAACGTCGCCAGAAGTACAGAACTTAATCAAACCAGGCGAAAAATTGATTACAACCCATTGCTGTTA
CTGTGGCATGCAGTGCGGCATGCATATCCGGGTCGATGAGAAAACCGGGGCCGTCAAAGGCGTTGAACCACGCTACGATT
TCCCGGTCAATAAAGGAAAACTTTGTCCGAAAGGCGTCACGGCCTATCAAACGATCGAACATCCGGACCGCATCATGACT
CCGCTCATCAAACGGAACGGCAAGTTTGAGAAAGCGACATGGGAAGAAGCACTCGACGTGATCGAAGCAAACTTTAAACG
CATTCAACGAGAGCATGGGAAAGATGCGGTGTCCGTCTTCGGCGGCGTCTCGATGACGAATGAAAAATGTTACCTCGTCG
GGAAGTATGCGCGGGTCGCACTCGGCACACGGTTCATCGATTACAACGGTCGCTTCTGCATGTCGTCAGCTGCCGGCGGT
TTCTTAAAGACACTTGGGATTGACCGCGGAGCGACGCTTCCGTGGACGGAGTTCGAGCACGTCGACACATTCTTTGTCGC
CGGTTCGAATATCGCCGAATGTCATCCGACGACGACGCAACATATTTGGAAGGCGAAAGACAAAGGCGCGAAAATCATCG
TCGCCGACCCGCGTGAAATTCCGATGGCGCGCGTCGCCGATGTCCATCTCGATTTGAAGCCAGGGACGGATGCGGCGCTT
GCGATGGGACTGCTCAACTTTTTAGTCGAACACGATTATGTTGATCAAGCGTACGTGGATGAGCGTTGCGATAACTATGA
AGAGTTGAAAGCGTCGGTCCGAGACTGGACGCTCGAACGGACGAGTGAGGTGACCGGTGTCGCCATCGAAAAACTCGAGA
AGGCCGGTCATATTTATGGGAAGAGCCCACGTTCGGTCATGTTGTTCGCTCGCGGCGTCGAACAACAGTCAAAAGGCGTC
GACAATGTCTCGCTCTATACGTCGATGGCGCTCTTACGAGGCATGATTGGGAAGTTTGCTTCGGGCGTCGCCACGATGAC
GGGGCAAGGGAACGGGCAGGGTGGACGAGAACATGGACAAAAAGCAGACCTGTTACCAGGCTACCGAAAATTAACTGATC
CGAAAGCCATTGAATATGTGAGCGGGGTTTGGGGCATCGATCCTTCCGAGATGCCAAAACCAGGTGTGTCCGCGTTCGAA
ATGTTCGATGAGATTCAAGCGGGAACGATTCGGGCGATGCACCTCATGTGTTCGAACCCAGCCGTTTCGGCACCGAACCT
TCCGTACGTGTGGGACAGTTTCAACAAACTCGATTTCTTCGTCGTTAGTGACTTCTTCATGTCGGAATCTTGTGAGTTCG
CTGATGTCGTCTTACCGGCTTGTTCGTGGGCCGAAGACGAAGGGACGACGACAAACTTAGAAGGACGAGTCATTCGGATC
CGGAAAGTGCGCGAACCGCTCGGGGATTCGCTTCCAGACTGGGAAATCATGAAGCGGATCGCTGAGCGGATGGGACGCGG
ACAGTACTTCCCGTATGAGACGGCGCGTGACATCTGGGAAGAGTTCCGCATCGCGACGAAAGGTGGAGCGGCTGACTACT
CTGGGATCACATGGGAACGAATCGACGACGAGTATGGTGTCTTTTGGCCATGTCCGTCCGAAGATCATCCCGGCACACCG
ACGATGTTCAAAGAACGGTTTGGAACGGCGAATGGAAAAGCAAATTTGCCTGTCGTCACATATCGGCCGGCCGGTGAATA
TGCGAATGAAGAATATCCGGTCACGCTCACGACAGGTCGGGTCGTCTTTCATTACTTATCGGGCAATCAGACGCGCCGTG
TCGACTTCTTGAGCACGATGTCAAAACTGCCGTTCGTGGAGATCCATCCGGAGTTAGCATCAAGTTATGGCCTTCAAACG
GGCGACAAAGTCGAGCTTCGCACACGTCGCGGGTCGATTGTCGTCGAAGCACGTTTGACGAAGGCGATTCGAAAAGATAC
GGTCTTCGTCCCTTATCATTGGGGCAAGGAGCTTGCCATCAATCAATTGACGAACCCGTGTCTCGATCCGATGTCACGCA
TGCCGGAGTTCAAGGTATGTGCGGTACAAATGAAAAAGTTACCGGCCAACACCCCGTTACACATTAATCCGTTGACGGTC
CCGCTCGGATCAAGCGTGCCGTTCATGGCGGACCATGCGATTGCGACGAGCTGTCACGTCATGGATGAGGAGGTTCATAC
GAAATGA

Upstream 100 bases:

>100_bases
GGAAGTGACGGGATGCATTATCCGCTTCAAACGATTAATGGCTATCACGTCAGTGGATTTTCAAAACGAAGAGCGGAAAC
GTTCTATGGAGGGGAAGACG

Downstream 100 bases:

>100_bases
ATAAGGTCATGTATTTAGAGTTTGAACGTTGTATCGGCTGTCGGGCCTGTCAGGCAGCTTGCCGCGAATGCGGGGACCAC
GAGGGGCTCGAGCGCAACTA

Product: formate dehydrogenase, subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 748; Mature: 747

Protein sequence:

>748_residues
MSDFLTSPEVQNLIKPGEKLITTHCCYCGMQCGMHIRVDEKTGAVKGVEPRYDFPVNKGKLCPKGVTAYQTIEHPDRIMT
PLIKRNGKFEKATWEEALDVIEANFKRIQREHGKDAVSVFGGVSMTNEKCYLVGKYARVALGTRFIDYNGRFCMSSAAGG
FLKTLGIDRGATLPWTEFEHVDTFFVAGSNIAECHPTTTQHIWKAKDKGAKIIVADPREIPMARVADVHLDLKPGTDAAL
AMGLLNFLVEHDYVDQAYVDERCDNYEELKASVRDWTLERTSEVTGVAIEKLEKAGHIYGKSPRSVMLFARGVEQQSKGV
DNVSLYTSMALLRGMIGKFASGVATMTGQGNGQGGREHGQKADLLPGYRKLTDPKAIEYVSGVWGIDPSEMPKPGVSAFE
MFDEIQAGTIRAMHLMCSNPAVSAPNLPYVWDSFNKLDFFVVSDFFMSESCEFADVVLPACSWAEDEGTTTNLEGRVIRI
RKVREPLGDSLPDWEIMKRIAERMGRGQYFPYETARDIWEEFRIATKGGAADYSGITWERIDDEYGVFWPCPSEDHPGTP
TMFKERFGTANGKANLPVVTYRPAGEYANEEYPVTLTTGRVVFHYLSGNQTRRVDFLSTMSKLPFVEIHPELASSYGLQT
GDKVELRTRRGSIVVEARLTKAIRKDTVFVPYHWGKELAINQLTNPCLDPMSRMPEFKVCAVQMKKLPANTPLHINPLTV
PLGSSVPFMADHAIATSCHVMDEEVHTK

Sequences:

>Translated_748_residues
MSDFLTSPEVQNLIKPGEKLITTHCCYCGMQCGMHIRVDEKTGAVKGVEPRYDFPVNKGKLCPKGVTAYQTIEHPDRIMT
PLIKRNGKFEKATWEEALDVIEANFKRIQREHGKDAVSVFGGVSMTNEKCYLVGKYARVALGTRFIDYNGRFCMSSAAGG
FLKTLGIDRGATLPWTEFEHVDTFFVAGSNIAECHPTTTQHIWKAKDKGAKIIVADPREIPMARVADVHLDLKPGTDAAL
AMGLLNFLVEHDYVDQAYVDERCDNYEELKASVRDWTLERTSEVTGVAIEKLEKAGHIYGKSPRSVMLFARGVEQQSKGV
DNVSLYTSMALLRGMIGKFASGVATMTGQGNGQGGREHGQKADLLPGYRKLTDPKAIEYVSGVWGIDPSEMPKPGVSAFE
MFDEIQAGTIRAMHLMCSNPAVSAPNLPYVWDSFNKLDFFVVSDFFMSESCEFADVVLPACSWAEDEGTTTNLEGRVIRI
RKVREPLGDSLPDWEIMKRIAERMGRGQYFPYETARDIWEEFRIATKGGAADYSGITWERIDDEYGVFWPCPSEDHPGTP
TMFKERFGTANGKANLPVVTYRPAGEYANEEYPVTLTTGRVVFHYLSGNQTRRVDFLSTMSKLPFVEIHPELASSYGLQT
GDKVELRTRRGSIVVEARLTKAIRKDTVFVPYHWGKELAINQLTNPCLDPMSRMPEFKVCAVQMKKLPANTPLHINPLTV
PLGSSVPFMADHAIATSCHVMDEEVHTK
>Mature_747_residues
SDFLTSPEVQNLIKPGEKLITTHCCYCGMQCGMHIRVDEKTGAVKGVEPRYDFPVNKGKLCPKGVTAYQTIEHPDRIMTP
LIKRNGKFEKATWEEALDVIEANFKRIQREHGKDAVSVFGGVSMTNEKCYLVGKYARVALGTRFIDYNGRFCMSSAAGGF
LKTLGIDRGATLPWTEFEHVDTFFVAGSNIAECHPTTTQHIWKAKDKGAKIIVADPREIPMARVADVHLDLKPGTDAALA
MGLLNFLVEHDYVDQAYVDERCDNYEELKASVRDWTLERTSEVTGVAIEKLEKAGHIYGKSPRSVMLFARGVEQQSKGVD
NVSLYTSMALLRGMIGKFASGVATMTGQGNGQGGREHGQKADLLPGYRKLTDPKAIEYVSGVWGIDPSEMPKPGVSAFEM
FDEIQAGTIRAMHLMCSNPAVSAPNLPYVWDSFNKLDFFVVSDFFMSESCEFADVVLPACSWAEDEGTTTNLEGRVIRIR
KVREPLGDSLPDWEIMKRIAERMGRGQYFPYETARDIWEEFRIATKGGAADYSGITWERIDDEYGVFWPCPSEDHPGTPT
MFKERFGTANGKANLPVVTYRPAGEYANEEYPVTLTTGRVVFHYLSGNQTRRVDFLSTMSKLPFVEIHPELASSYGLQTG
DKVELRTRRGSIVVEARLTKAIRKDTVFVPYHWGKELAINQLTNPCLDPMSRMPEFKVCAVQMKKLPANTPLHINPLTVP
LGSSVPFMADHAIATSCHVMDEEVHTK

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=699, Percent_Identity=31.9027181688126, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788534, Length=791, Percent_Identity=26.6750948166877, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI3868720, Length=616, Percent_Identity=24.1883116883117, Blast_Score=130, Evalue=5e-31,
Organism=Escherichia coli, GI3868719, Length=623, Percent_Identity=24.2375601926164, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI87081797, Length=737, Percent_Identity=24.2876526458616, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1787870, Length=733, Percent_Identity=22.3738062755798, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI171474008, Length=729, Percent_Identity=22.3593964334705, Blast_Score=109, Evalue=8e-25,
Organism=Escherichia coli, GI1787778, Length=384, Percent_Identity=22.1354166666667, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 83449; Mature: 83318

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFLTSPEVQNLIKPGEKLITTHCCYCGMQCGMHIRVDEKTGAVKGVEPRYDFPVNKGK
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCC
LCPKGVTAYQTIEHPDRIMTPLIKRNGKFEKATWEEALDVIEANFKRIQREHGKDAVSVF
CCCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GGVSMTNEKCYLVGKYARVALGTRFIDYNGRFCMSSAAGGFLKTLGIDRGATLPWTEFEH
CCCEECCCCEEEEEHHHHHHHCEEEEECCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCC
VDTFFVAGSNIAECHPTTTQHIWKAKDKGAKIIVADPREIPMARVADVHLDLKPGTDAAL
CCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEEECCCCHHHH
AMGLLNFLVEHDYVDQAYVDERCDNYEELKASVRDWTLERTSEVTGVAIEKLEKAGHIYG
HHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSPRSVMLFARGVEQQSKGVDNVSLYTSMALLRGMIGKFASGVATMTGQGNGQGGREHGQ
CCCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHCCC
KADLLPGYRKLTDPKAIEYVSGVWGIDPSEMPKPGVSAFEMFDEIQAGTIRAMHLMCSNP
CCCCCCCHHHCCCCHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCCCEEEEEEEECCC
AVSAPNLPYVWDSFNKLDFFVVSDFFMSESCEFADVVLPACSWAEDEGTTTNLEGRVIRI
CCCCCCCCHHCCCCCCEEEEEEEHHHHCCCCCHHHHHHHCCCCCCCCCCEECCCCCEEEE
RKVREPLGDSLPDWEIMKRIAERMGRGQYFPYETARDIWEEFRIATKGGAADYSGITWER
EHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCEE
IDDEYGVFWPCPSEDHPGTPTMFKERFGTANGKANLPVVTYRPAGEYANEEYPVTLTTGR
ECCCCCEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECE
VVFHYLSGNQTRRVDFLSTMSKLPFVEIHPELASSYGLQTGDKVELRTRRGSIVVEARLT
EEEEEECCCCCEEHHHHHHHHHCCEEEECHHHHHHCCCCCCCEEEEEECCCCEEEEHHHH
KAIRKDTVFVPYHWGKELAINQLTNPCLDPMSRMPEFKVCAVQMKKLPANTPLHINPLTV
HHHHHCEEEEEECCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCEEE
PLGSSVPFMADHAIATSCHVMDEEVHTK
ECCCCCCCHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
SDFLTSPEVQNLIKPGEKLITTHCCYCGMQCGMHIRVDEKTGAVKGVEPRYDFPVNKGK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCC
LCPKGVTAYQTIEHPDRIMTPLIKRNGKFEKATWEEALDVIEANFKRIQREHGKDAVSVF
CCCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GGVSMTNEKCYLVGKYARVALGTRFIDYNGRFCMSSAAGGFLKTLGIDRGATLPWTEFEH
CCCEECCCCEEEEEHHHHHHHCEEEEECCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCC
VDTFFVAGSNIAECHPTTTQHIWKAKDKGAKIIVADPREIPMARVADVHLDLKPGTDAAL
CCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEEECCCCHHHH
AMGLLNFLVEHDYVDQAYVDERCDNYEELKASVRDWTLERTSEVTGVAIEKLEKAGHIYG
HHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSPRSVMLFARGVEQQSKGVDNVSLYTSMALLRGMIGKFASGVATMTGQGNGQGGREHGQ
CCCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHCCC
KADLLPGYRKLTDPKAIEYVSGVWGIDPSEMPKPGVSAFEMFDEIQAGTIRAMHLMCSNP
CCCCCCCHHHCCCCHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCCCEEEEEEEECCC
AVSAPNLPYVWDSFNKLDFFVVSDFFMSESCEFADVVLPACSWAEDEGTTTNLEGRVIRI
CCCCCCCCHHCCCCCCEEEEEEEHHHHCCCCCHHHHHHHCCCCCCCCCCEECCCCCEEEE
RKVREPLGDSLPDWEIMKRIAERMGRGQYFPYETARDIWEEFRIATKGGAADYSGITWER
EHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCEE
IDDEYGVFWPCPSEDHPGTPTMFKERFGTANGKANLPVVTYRPAGEYANEEYPVTLTTGR
ECCCCCEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECE
VVFHYLSGNQTRRVDFLSTMSKLPFVEIHPELASSYGLQTGDKVELRTRRGSIVVEARLT
EEEEEECCCCCEEHHHHHHHHHCCEEEECHHHHHHCCCCCCCEEEEEECCCCEEEEHHHH
KAIRKDTVFVPYHWGKELAINQLTNPCLDPMSRMPEFKVCAVQMKKLPANTPLHINPLTV
HHHHHCEEEEEECCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCEEE
PLGSSVPFMADHAIATSCHVMDEEVHTK
ECCCCCCCHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]