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The map label for this gene is serS

Identifier: 229917440

GI number: 229917440

Start: 1673691

End: 1674974

Strand: Reverse

Name: serS

Synonym: EAT1b_1715

Alternate gene names: 229917440

Gene position: 1674974-1673691 (Counterclockwise)

Preceding gene: 229917441

Following gene: 229917437

Centisome position: 55.83

GC content: 48.05

Gene sequence:

>1284_bases
ATGTTAGACATTAAACGTTTGCGACAGGATTTTGAAGGAATCAAAGAAAAGTTAGCTCACCGGGGGGAAGACTTGAGTGC
ACTTGACCGCTTCCCACAACTAGAGGAGAAACGTCGTGACTTGATCAACGAAGTGGAAGTGAAGAAAGCGAAGCGCAACG
AAGCGACAAAACAAATCGCTGAATTGAAGCGGAACAAAGAAGATGCCGAAGGCCCGATCTTAGAGACACGTCGCCTCGGA
GATGAAATCAAACTGTTGGACGAAGAGTTACGCGAAGTTGAGGCAGAACTCAACTTGATTTTACTCAGCATCCCAAATAT
CCCGCACGAATCAACACCAATCGGCGAAACGGAAGACGACAACGTGACGATTCGCGAAATCGGGACAAAGCCCGAGTTTG
ATTTCGAGCCCAAGCAACATTGGGACGTGATGGAAGACCTTCAAATCGTCGATGTCGAGCGCGCTGGAAAAGTAACGGGT
AGCCGCTTCGTCTTCTATAAAGGATTAGGCGCTCGCCTAGAACGTGCCCTGATCAACTTTATGATGGACCTTCATTCAGA
TGAGCATGGGTACGCTGAAGTGTTACCTCCTTACATGGTCAATCGTGATTCAATGACAGGAACGGGTCAGCTTCCTAAGT
TCGAAGAAGATGCATTTAAAGTAGCGGACACGAACTACTTCCTCGTTCCGACGGCTGAAGTACCGGTCACGAACATGCAC
CGTGAAGAGATTCTTCCGGAGACACAACTTCCAATCGCGTATACAGCATATAGCGCAAACTTCCGTTCTGAAGCTGGGTC
AGCGGGACGTGACACACGTGGATTAATTCGTCAGCACCAATTCAATAAAGTCGAGCTCGTCCGCTTCGTAAAACCGGAAG
AGTCTTATGAGCAATTGGAACTGTTGACAGGTCATGCTGAAGAAGTCTTAAAACGTCTAGGCCTTCCTTATCAAGTGCTC
AGCATGTGTACGGCAGATCTCGGATTCACTGCCGCGAAAAAATACGATATTGAAGTATGGATGCCGGCACAAGGGGTATA
TCGTGAAATCTCGTCTTGCTCAAACTTTGAAGATTTCCAAGCGCGTCGTGCGGGAATTCGTTTCCGTCGTGACGCCAATG
CGAAACCTGAGTTCGTGCATACGTTGAACGGTTCAGGTCTAGCGGTCGGACGGACGGTCGCAGCGATTCTAGAAAATTAT
CAACAAGCGGATGGATCGGTCGTGATCCCAGAAGTTCTTCGTCCTTACATGGGTGGGAAAGAAAAGATTGAAATGCCGAA
TTAA

Upstream 100 bases:

>100_bases
TTTGAGCAACGAGGGTGGCAACGCGGATCCAAGGTGGTTCGTCCCTTTCCTACGGGAAAGGGACGTGCCACCTTTTTTAT
ATGAAAAAGGAGGAATAGCA

Downstream 100 bases:

>100_bases
ACGCTTGAGCAGCCTCGATTTTGAGGCTGCTCAATTTTTTTAATGTTTTTTCACAAAAGGGTTTGACATCTGTCTCTTCA
GTTGGTACTATAAATCATGT

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLDIKRLRQDFEGIKEKLAHRGEDLSALDRFPQLEEKRRDLINEVEVKKAKRNEATKQIAELKRNKEDAEGPILETRRLG
DEIKLLDEELREVEAELNLILLSIPNIPHESTPIGETEDDNVTIREIGTKPEFDFEPKQHWDVMEDLQIVDVERAGKVTG
SRFVFYKGLGARLERALINFMMDLHSDEHGYAEVLPPYMVNRDSMTGTGQLPKFEEDAFKVADTNYFLVPTAEVPVTNMH
REEILPETQLPIAYTAYSANFRSEAGSAGRDTRGLIRQHQFNKVELVRFVKPEESYEQLELLTGHAEEVLKRLGLPYQVL
SMCTADLGFTAAKKYDIEVWMPAQGVYREISSCSNFEDFQARRAGIRFRRDANAKPEFVHTLNGSGLAVGRTVAAILENY
QQADGSVVIPEVLRPYMGGKEKIEMPN

Sequences:

>Translated_427_residues
MLDIKRLRQDFEGIKEKLAHRGEDLSALDRFPQLEEKRRDLINEVEVKKAKRNEATKQIAELKRNKEDAEGPILETRRLG
DEIKLLDEELREVEAELNLILLSIPNIPHESTPIGETEDDNVTIREIGTKPEFDFEPKQHWDVMEDLQIVDVERAGKVTG
SRFVFYKGLGARLERALINFMMDLHSDEHGYAEVLPPYMVNRDSMTGTGQLPKFEEDAFKVADTNYFLVPTAEVPVTNMH
REEILPETQLPIAYTAYSANFRSEAGSAGRDTRGLIRQHQFNKVELVRFVKPEESYEQLELLTGHAEEVLKRLGLPYQVL
SMCTADLGFTAAKKYDIEVWMPAQGVYREISSCSNFEDFQARRAGIRFRRDANAKPEFVHTLNGSGLAVGRTVAAILENY
QQADGSVVIPEVLRPYMGGKEKIEMPN
>Mature_427_residues
MLDIKRLRQDFEGIKEKLAHRGEDLSALDRFPQLEEKRRDLINEVEVKKAKRNEATKQIAELKRNKEDAEGPILETRRLG
DEIKLLDEELREVEAELNLILLSIPNIPHESTPIGETEDDNVTIREIGTKPEFDFEPKQHWDVMEDLQIVDVERAGKVTG
SRFVFYKGLGARLERALINFMMDLHSDEHGYAEVLPPYMVNRDSMTGTGQLPKFEEDAFKVADTNYFLVPTAEVPVTNMH
REEILPETQLPIAYTAYSANFRSEAGSAGRDTRGLIRQHQFNKVELVRFVKPEESYEQLELLTGHAEEVLKRLGLPYQVL
SMCTADLGFTAAKKYDIEVWMPAQGVYREISSCSNFEDFQARRAGIRFRRDANAKPEFVHTLNGSGLAVGRTVAAILENY
QQADGSVVIPEVLRPYMGGKEKIEMPN

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI16306548, Length=365, Percent_Identity=37.5342465753425, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI8923421, Length=448, Percent_Identity=32.3660714285714, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI225579074, Length=452, Percent_Identity=31.858407079646, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1787120, Length=430, Percent_Identity=53.2558139534884, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17539260, Length=335, Percent_Identity=35.2238805970149, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17542084, Length=319, Percent_Identity=30.4075235109718, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320226, Length=445, Percent_Identity=33.9325842696629, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321799, Length=294, Percent_Identity=38.0952380952381, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24581334, Length=367, Percent_Identity=35.149863760218, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI17737985, Length=378, Percent_Identity=33.0687830687831, Blast_Score=178, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_EXISA (C4KZX8)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886086.1
- GeneID:   7870759
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1715
- OMA:   AHWDLGP
- ProtClustDB:   PRK05431
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 48696; Mature: 48696

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 285-285 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIKRLRQDFEGIKEKLAHRGEDLSALDRFPQLEEKRRDLINEVEVKKAKRNEATKQIA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKRNKEDAEGPILETRRLGDEIKLLDEELREVEAELNLILLSIPNIPHESTPIGETEDD
HHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCC
NVTIREIGTKPEFDFEPKQHWDVMEDLQIVDVERAGKVTGSRFVFYKGLGARLERALINF
CEEEEECCCCCCCCCCCHHHCCHHHCCEEEEHHHCCCCCCCEEEEECCHHHHHHHHHHHH
MMDLHSDEHGYAEVLPPYMVNRDSMTGTGQLPKFEEDAFKVADTNYFLVPTAEVPVTNMH
HHHHCCCCCCHHHHCCCHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCH
REEILPETQLPIAYTAYSANFRSEAGSAGRDTRGLIRQHQFNKVELVRFVKPEESYEQLE
HHHCCCCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHCCCCEEEHEECCCCHHHHHHH
LLTGHAEEVLKRLGLPYQVLSMCTADLGFTAAKKYDIEVWMPAQGVYREISSCSNFEDFQ
HHHCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHHCCCHHHHH
ARRAGIRFRRDANAKPEFVHTLNGSGLAVGRTVAAILENYQQADGSVVIPEVLRPYMGGK
HHHHCCEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCC
EKIEMPN
CCCCCCC
>Mature Secondary Structure
MLDIKRLRQDFEGIKEKLAHRGEDLSALDRFPQLEEKRRDLINEVEVKKAKRNEATKQIA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKRNKEDAEGPILETRRLGDEIKLLDEELREVEAELNLILLSIPNIPHESTPIGETEDD
HHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCC
NVTIREIGTKPEFDFEPKQHWDVMEDLQIVDVERAGKVTGSRFVFYKGLGARLERALINF
CEEEEECCCCCCCCCCCHHHCCHHHCCEEEEHHHCCCCCCCEEEEECCHHHHHHHHHHHH
MMDLHSDEHGYAEVLPPYMVNRDSMTGTGQLPKFEEDAFKVADTNYFLVPTAEVPVTNMH
HHHHCCCCCCHHHHCCCHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCH
REEILPETQLPIAYTAYSANFRSEAGSAGRDTRGLIRQHQFNKVELVRFVKPEESYEQLE
HHHCCCCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHCCCCEEEHEECCCCHHHHHHH
LLTGHAEEVLKRLGLPYQVLSMCTADLGFTAAKKYDIEVWMPAQGVYREISSCSNFEDFQ
HHHCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHHCCCHHHHH
ARRAGIRFRRDANAKPEFVHTLNGSGLAVGRTVAAILENYQQADGSVVIPEVLRPYMGGK
HHHHCCEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCC
EKIEMPN
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA