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The map label for this gene is dacA [H]

Identifier: 229917441

GI number: 229917441

Start: 1675253

End: 1676500

Strand: Reverse

Name: dacA [H]

Synonym: EAT1b_1716

Alternate gene names: 229917441

Gene position: 1676500-1675253 (Counterclockwise)

Preceding gene: 229917442

Following gene: 229917440

Centisome position: 55.89

GC content: 51.68

Gene sequence:

>1248_bases
GTGAAGGCCCGTTATTTCATCATATTGCTCACTGTCGTATTCGTCATACAGACCATGTTGCCATCGGCTGCCTCTGCGGC
CGCTCCGAAAGTGGATGCAGCAAGCGCGATGCTTGTAGACGTGACGACAGGACAAGTTCTATTTGCCAAACAAGAAGATT
CGATGCTTCAGCCGGCTTCAATCACGAAGCTGATGACTGCATTTTTGACGCGTGAGGCGATTGAAGCAGGGAAACTCAGT
TGGACACAAGAAATCACACCGAGCGATGCTGCTTTGGCGTTGACGAGAAAGCCGGGGTTGGCACGAATCCCGCTGTTAAA
TAAACCATATACCGTCAAAGAGTTATACGATGCCGCGTTGATTCGTTCAGCGAATGAAGCGGCCGTAACGCTCGGGGAGG
CCGTATCCGGTTCGGAAGAGGCATTTGTCAAAGAGATGAATCGTCGGGCCGATGAACTCGGGATGACCCAAACGACGTTC
GCGAACGCCTCAGGACTCGATTCCGAATCTGCAGGTCTACCAGGAGACAACTTGACGTCTGCTAAGGACTTGATGAAGCT
CGCGATCGCGTATTTGACGACGTATCCGGAAGTGCTCGACGTGACGAAGCGGGCATACGTGGACGTCGACGGTGTTCGAT
TTGATGCGACGAATCGCATGCTCGCTAATCGTGACCTCGCTTATCCGGGAATGCTCGGCTTCAAGACAGGAACGACGAAT
GAGGCGGGATATTGTTTCATCGGCGTATCTACACGGGATGGGCGTACCGTTTTGTCGGTCGTCCTCGGTGCAACATCGGA
CGAGGGACGTTATGCAGCAACGAAAGCGCTACATGATTATGCCTATGCAGATTTCGTGATGACCCCAATCTTGCGGATGG
GGGAGACAATCTCCAAGATGGCAACGGTCTCTCAAGGCGAGGAAGAGCAAGTCGCGGTCCGAACGACGAACGACTTTGAA
GTATTAGTCGAAAAAGGAAGACCCGTACCGGAGCCCGAATATGATCTGCTTGATTTAGAGGCTCCGATTAAGGCTGACAC
ACAGGCAGGCATGGTCACCATCGATTCTTCGGAGAACGTACGCTATTTAGAGGGAGAGGCTCCTCCTAAGATGGCACTTG
TTACAGCGGAGTCGGTCGATACCGCCTCGTTCTTGACGAGAATAACACGAGCTTTTTTGGATTTCCTCGATCAAATCCGA
CTCGCACTTGGAAAAATCAGTCTTGTCGAACAAGTTGAGATGTTGTAA

Upstream 100 bases:

>100_bases
AGTCAACATGTATAAGTTTGAGGGACCTCGCAAAAAGGGGGTCCTTCAAACTTATTTTTTGTTACACTGAACAAAGAAGC
GTACAGAAAGGGGAAACGAT

Downstream 100 bases:

>100_bases
AGTAAGATTAATTCAATGACAGATGCGATGATGAGAATAAGTACGTTACGTACGTCTACTGACAGAGAGCTAGTGGTTGG
TGCAAACTAGTGAGATGACG

Product: peptidase S11 D-alanyl-D-alanine carboxypeptidase 1

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKARYFIILLTVVFVIQTMLPSAASAAAPKVDAASAMLVDVTTGQVLFAKQEDSMLQPASITKLMTAFLTREAIEAGKLS
WTQEITPSDAALALTRKPGLARIPLLNKPYTVKELYDAALIRSANEAAVTLGEAVSGSEEAFVKEMNRRADELGMTQTTF
ANASGLDSESAGLPGDNLTSAKDLMKLAIAYLTTYPEVLDVTKRAYVDVDGVRFDATNRMLANRDLAYPGMLGFKTGTTN
EAGYCFIGVSTRDGRTVLSVVLGATSDEGRYAATKALHDYAYADFVMTPILRMGETISKMATVSQGEEEQVAVRTTNDFE
VLVEKGRPVPEPEYDLLDLEAPIKADTQAGMVTIDSSENVRYLEGEAPPKMALVTAESVDTASFLTRITRAFLDFLDQIR
LALGKISLVEQVEML

Sequences:

>Translated_415_residues
MKARYFIILLTVVFVIQTMLPSAASAAAPKVDAASAMLVDVTTGQVLFAKQEDSMLQPASITKLMTAFLTREAIEAGKLS
WTQEITPSDAALALTRKPGLARIPLLNKPYTVKELYDAALIRSANEAAVTLGEAVSGSEEAFVKEMNRRADELGMTQTTF
ANASGLDSESAGLPGDNLTSAKDLMKLAIAYLTTYPEVLDVTKRAYVDVDGVRFDATNRMLANRDLAYPGMLGFKTGTTN
EAGYCFIGVSTRDGRTVLSVVLGATSDEGRYAATKALHDYAYADFVMTPILRMGETISKMATVSQGEEEQVAVRTTNDFE
VLVEKGRPVPEPEYDLLDLEAPIKADTQAGMVTIDSSENVRYLEGEAPPKMALVTAESVDTASFLTRITRAFLDFLDQIR
LALGKISLVEQVEML
>Mature_415_residues
MKARYFIILLTVVFVIQTMLPSAASAAAPKVDAASAMLVDVTTGQVLFAKQEDSMLQPASITKLMTAFLTREAIEAGKLS
WTQEITPSDAALALTRKPGLARIPLLNKPYTVKELYDAALIRSANEAAVTLGEAVSGSEEAFVKEMNRRADELGMTQTTF
ANASGLDSESAGLPGDNLTSAKDLMKLAIAYLTTYPEVLDVTKRAYVDVDGVRFDATNRMLANRDLAYPGMLGFKTGTTN
EAGYCFIGVSTRDGRTVLSVVLGATSDEGRYAATKALHDYAYADFVMTPILRMGETISKMATVSQGEEEQVAVRTTNDFE
VLVEKGRPVPEPEYDLLDLEAPIKADTQAGMVTIDSSENVRYLEGEAPPKMALVTAESVDTASFLTRITRAFLDFLDQIR
LALGKISLVEQVEML

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=374, Percent_Identity=30.7486631016043, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87082022, Length=408, Percent_Identity=27.4509803921569, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786851, Length=387, Percent_Identity=25.8397932816537, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87082054, Length=258, Percent_Identity=29.4573643410853, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44935; Mature: 44935

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKARYFIILLTVVFVIQTMLPSAASAAAPKVDAASAMLVDVTTGQVLFAKQEDSMLQPAS
CCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCHH
ITKLMTAFLTREAIEAGKLSWTQEITPSDAALALTRKPGLARIPLLNKPYTVKELYDAAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCCHHHHHHHHHH
IRSANEAAVTLGEAVSGSEEAFVKEMNRRADELGMTQTTFANASGLDSESAGLPGDNLTS
HHCCCHHHEEECHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCH
AKDLMKLAIAYLTTYPEVLDVTKRAYVDVDGVRFDATNRMLANRDLAYPGMLGFKTGTTN
HHHHHHHHHHHHHHCHHHHHHHHHHEEEECCEEECCCCCHHHCCCCCCCCCEEECCCCCC
EAGYCFIGVSTRDGRTVLSVVLGATSDEGRYAATKALHDYAYADFVMTPILRMGETISKM
CCCEEEEEECCCCCHHEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATVSQGEEEQVAVRTTNDFEVLVEKGRPVPEPEYDLLDLEAPIKADTQAGMVTIDSSENV
HHHCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCE
RYLEGEAPPKMALVTAESVDTASFLTRITRAFLDFLDQIRLALGKISLVEQVEML
EEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKARYFIILLTVVFVIQTMLPSAASAAAPKVDAASAMLVDVTTGQVLFAKQEDSMLQPAS
CCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCHH
ITKLMTAFLTREAIEAGKLSWTQEITPSDAALALTRKPGLARIPLLNKPYTVKELYDAAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCCHHHHHHHHHH
IRSANEAAVTLGEAVSGSEEAFVKEMNRRADELGMTQTTFANASGLDSESAGLPGDNLTS
HHCCCHHHEEECHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCH
AKDLMKLAIAYLTTYPEVLDVTKRAYVDVDGVRFDATNRMLANRDLAYPGMLGFKTGTTN
HHHHHHHHHHHHHHCHHHHHHHHHHEEEECCEEECCCCCHHHCCCCCCCCCEEECCCCCC
EAGYCFIGVSTRDGRTVLSVVLGATSDEGRYAATKALHDYAYADFVMTPILRMGETISKM
CCCEEEEEECCCCCHHEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATVSQGEEEQVAVRTTNDFEVLVEKGRPVPEPEYDLLDLEAPIKADTQAGMVTIDSSENV
HHHCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCE
RYLEGEAPPKMALVTAESVDTASFLTRITRAFLDFLDQIRLALGKISLVEQVEML
EEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]