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The map label for this gene is thiD [H]

Identifier: 229917253

GI number: 229917253

Start: 1494723

End: 1495562

Strand: Reverse

Name: thiD [H]

Synonym: EAT1b_1528

Alternate gene names: 229917253

Gene position: 1495562-1494723 (Counterclockwise)

Preceding gene: 229917254

Following gene: 229917252

Centisome position: 49.85

GC content: 50.6

Gene sequence:

>840_bases
ATGAGACCGCAAGTATTGACGATTGCAGGATCTGATTCAGGGGGCGGAGCTGGTATTCAAGCCGATCTAAAAACGTTTCA
AGAGCTCGGTGTGTTCGGGACGAGTGTGTTGACTGCGATTACCGCCCAAAACACACTTGGGGTCCATGGAATCGAGGCGA
TTTCCACGGAAATGATTATCCAACAAATTGATGCGGTAGCGGATGATTTTAACATCACCGCCTGTAAAACTGGGATGTTG
GTGGATGCGGAGCGGATTGAAGCGGTAGCGAATGCGCTTGAGCGCCACCGTTTCGAGCATGTGGTGGTCGATCCGGTCAT
GGTGGCGAAAGGTGGCGCGTCCTTGTTGCAAGTTAGTGCAGTCGAAGCACTGCGCACGAAGCTAATACCACAGGCGACCA
TCGTGACCCCGAATATTCCTGAAGCAGAAGTGCTGGCAGGAATGGAAATTCGAACGGAGGCAGACCGTATGAAAGCCGCA
CAGGCGATTCGAGCACTTGGTGTCGATACCGTCATTATCAAAGGTGGGCATACGGATGATGTGGAAGCCGTTGATTTGAT
TATGAGCGAGACTGAAACGTTCTGTGTAAAAGCACCTAGAATCCAAACGAAAGATACGCACGGGACGGGATGTACGTTCT
CTGCAGCTTTGACGGCCGAACTGGCATATGGGAAGACATTGCGTCAAGCAGTCGTAGATGCAAAACAATTTATCCATCAG
GCGATTGCACATGGATTATCGATTGGACAGGGGCATGGACCGACGAATCATTGGGGACATCGTTTAGCGAAAGAAGAGGT
GATTACCGTTGACGTTCTGTACGAAACAAGCGCTCGCTAA

Upstream 100 bases:

>100_bases
GGGCAGAAGGGCCGGGAGACTTCCCGATTCACTTTTTAAATGCACTCCATCAATTGACGCAATATACAATCCCGACGAAT
CGAATCGAGGTGTCGACATC

Downstream 100 bases:

>100_bases
GCAGCTTCGCCTTTACTTTGTGTGTGGAACGACGGACGCGTCCGATTTGGTAGCGACCGTCAAAACGGCTCTTCTATGTG
GTGTGACATGTTTTCAATTT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRPQVLTIAGSDSGGGAGIQADLKTFQELGVFGTSVLTAITAQNTLGVHGIEAISTEMIIQQIDAVADDFNITACKTGML
VDAERIEAVANALERHRFEHVVVDPVMVAKGGASLLQVSAVEALRTKLIPQATIVTPNIPEAEVLAGMEIRTEADRMKAA
QAIRALGVDTVIIKGGHTDDVEAVDLIMSETETFCVKAPRIQTKDTHGTGCTFSAALTAELAYGKTLRQAVVDAKQFIHQ
AIAHGLSIGQGHGPTNHWGHRLAKEEVITVDVLYETSAR

Sequences:

>Translated_279_residues
MRPQVLTIAGSDSGGGAGIQADLKTFQELGVFGTSVLTAITAQNTLGVHGIEAISTEMIIQQIDAVADDFNITACKTGML
VDAERIEAVANALERHRFEHVVVDPVMVAKGGASLLQVSAVEALRTKLIPQATIVTPNIPEAEVLAGMEIRTEADRMKAA
QAIRALGVDTVIIKGGHTDDVEAVDLIMSETETFCVKAPRIQTKDTHGTGCTFSAALTAELAYGKTLRQAVVDAKQFIHQ
AIAHGLSIGQGHGPTNHWGHRLAKEEVITVDVLYETSAR
>Mature_279_residues
MRPQVLTIAGSDSGGGAGIQADLKTFQELGVFGTSVLTAITAQNTLGVHGIEAISTEMIIQQIDAVADDFNITACKTGML
VDAERIEAVANALERHRFEHVVVDPVMVAKGGASLLQVSAVEALRTKLIPQATIVTPNIPEAEVLAGMEIRTEADRMKAA
QAIRALGVDTVIIKGGHTDDVEAVDLIMSETETFCVKAPRIQTKDTHGTGCTFSAALTAELAYGKTLRQAVVDAKQFIHQ
AIAHGLSIGQGHGPTNHWGHRLAKEEVITVDVLYETSAR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=44.4015444015444, Blast_Score=218, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6325378, Length=271, Percent_Identity=33.5793357933579, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324517, Length=271, Percent_Identity=33.5793357933579, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324997, Length=271, Percent_Identity=33.9483394833948, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29610; Mature: 29610

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPQVLTIAGSDSGGGAGIQADLKTFQELGVFGTSVLTAITAQNTLGVHGIEAISTEMII
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
QQIDAVADDFNITACKTGMLVDAERIEAVANALERHRFEHVVVDPVMVAKGGASLLQVSA
HHHHHHHCCCCEEEECCCCEECHHHHHHHHHHHHHHCCCCEEECCEEEECCCHHHHHHHH
VEALRTKLIPQATIVTPNIPEAEVLAGMEIRTEADRMKAAQAIRALGVDTVIIKGGHTDD
HHHHHHHCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
VEAVDLIMSETETFCVKAPRIQTKDTHGTGCTFSAALTAELAYGKTLRQAVVDAKQFIHQ
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAHGLSIGQGHGPTNHWGHRLAKEEVITVDVLYETSAR
HHHHCCCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCC
>Mature Secondary Structure
MRPQVLTIAGSDSGGGAGIQADLKTFQELGVFGTSVLTAITAQNTLGVHGIEAISTEMII
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
QQIDAVADDFNITACKTGMLVDAERIEAVANALERHRFEHVVVDPVMVAKGGASLLQVSA
HHHHHHHCCCCEEEECCCCEECHHHHHHHHHHHHHHCCCCEEECCEEEECCCHHHHHHHH
VEALRTKLIPQATIVTPNIPEAEVLAGMEIRTEADRMKAAQAIRALGVDTVIIKGGHTDD
HHHHHHHCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
VEAVDLIMSETETFCVKAPRIQTKDTHGTGCTFSAALTAELAYGKTLRQAVVDAKQFIHQ
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAHGLSIGQGHGPTNHWGHRLAKEEVITVDVLYETSAR
HHHHCCCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]