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The map label for this gene is thiE [H]

Identifier: 229917252

GI number: 229917252

Start: 1494113

End: 1494754

Strand: Reverse

Name: thiE [H]

Synonym: EAT1b_1527

Alternate gene names: 229917252

Gene position: 1494754-1494113 (Counterclockwise)

Preceding gene: 229917253

Following gene: 229917251

Centisome position: 49.83

GC content: 52.96

Gene sequence:

>642_bases
TTGACGTTCTGTACGAAACAAGCGCTCGCTAAGCAGCTTCGCCTTTACTTTGTGTGTGGAACGACGGACGCGTCCGATTT
GGTAGCGACCGTCAAAACGGCTCTTCTATGTGGTGTGACATGTTTTCAATTTCGCGAAAAAGGTAAAGATGCATTGTATG
GAGATGAAAAAGAAGCGATGGCAAGGACGCTCCACCGTCTGTGTCGAGAAGCGGGTGTTCCGTTCATCGTCAATGACGAC
GTCGACTTGGCGCTCGCGATTGACGCGGATGGCATCCACGTTGGACAGGATGACTTACCTGCCAAACAGGTTCGAGAACG
GCTAGGACCAGATAAATGGTTGGGGGTGTCTGTCCATACGATGGAGGAAGTCAGAACGGCTCTTCCATTTGCAGATTATG
TCGGCATCGGTCCGATTCATGAGACGCAAAGTAAGTTGGATGCAGGACAAGTGCGTGGTACGGAACTGATTCAACAAGTT
CGCCGACACCACCCACTGCTTCCGATTGTGGGGATCGGGGGCATTCGTCCAGAGCATGTCCCTGCTATTATGGCTGACGG
GGCTGACGGTGTTGCCGTCATCTCTGCCATCGCTTCTGCACCGGATGTAGCAACGGCTACCCGGCGTTTCGCTTGTCTCT
GA

Upstream 100 bases:

>100_bases
TTTATCCATCAGGCGATTGCACATGGATTATCGATTGGACAGGGGCATGGACCGACGAATCATTGGGGACATCGTTTAGC
GAAAGAAGAGGTGATTACCG

Downstream 100 bases:

>100_bases
ATTCAATTTTTGAGGAGGAAGACAGATGAACGTATTTGTTATCGGTGCAAACGGTCAGATTGGCCGTCAGTTTGTCGAAA
AATTACACAATGAAGGGAAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTFCTKQALAKQLRLYFVCGTTDASDLVATVKTALLCGVTCFQFREKGKDALYGDEKEAMARTLHRLCREAGVPFIVNDD
VDLALAIDADGIHVGQDDLPAKQVRERLGPDKWLGVSVHTMEEVRTALPFADYVGIGPIHETQSKLDAGQVRGTELIQQV
RRHHPLLPIVGIGGIRPEHVPAIMADGADGVAVISAIASAPDVATATRRFACL

Sequences:

>Translated_213_residues
MTFCTKQALAKQLRLYFVCGTTDASDLVATVKTALLCGVTCFQFREKGKDALYGDEKEAMARTLHRLCREAGVPFIVNDD
VDLALAIDADGIHVGQDDLPAKQVRERLGPDKWLGVSVHTMEEVRTALPFADYVGIGPIHETQSKLDAGQVRGTELIQQV
RRHHPLLPIVGIGGIRPEHVPAIMADGADGVAVISAIASAPDVATATRRFACL
>Mature_212_residues
TFCTKQALAKQLRLYFVCGTTDASDLVATVKTALLCGVTCFQFREKGKDALYGDEKEAMARTLHRLCREAGVPFIVNDDV
DLALAIDADGIHVGQDDLPAKQVRERLGPDKWLGVSVHTMEEVRTALPFADYVGIGPIHETQSKLDAGQVRGTELIQQVR
RHHPLLPIVGIGGIRPEHVPAIMADGADGVAVISAIASAPDVATATRRFACL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=195, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=219, Percent_Identity=33.7899543378995, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22984; Mature: 22853

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFCTKQALAKQLRLYFVCGTTDASDLVATVKTALLCGVTCFQFREKGKDALYGDEKEAM
CCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ARTLHRLCREAGVPFIVNDDVDLALAIDADGIHVGQDDLPAKQVRERLGPDKWLGVSVHT
HHHHHHHHHHCCCCEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHCCCCCEECCCHHH
MEEVRTALPFADYVGIGPIHETQSKLDAGQVRGTELIQQVRRHHPLLPIVGIGGIRPEHV
HHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCC
PAIMADGADGVAVISAIASAPDVATATRRFACL
CEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TFCTKQALAKQLRLYFVCGTTDASDLVATVKTALLCGVTCFQFREKGKDALYGDEKEAM
CCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ARTLHRLCREAGVPFIVNDDVDLALAIDADGIHVGQDDLPAKQVRERLGPDKWLGVSVHT
HHHHHHHHHHCCCCEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHCCCCCEECCCHHH
MEEVRTALPFADYVGIGPIHETQSKLDAGQVRGTELIQQVRRHHPLLPIVGIGGIRPEHV
HHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCC
PAIMADGADGVAVISAIASAPDVATATRRFACL
CEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA