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The map label for this gene is thiM

Identifier: 229917254

GI number: 229917254

Start: 1495559

End: 1496350

Strand: Reverse

Name: thiM

Synonym: EAT1b_1529

Alternate gene names: 229917254

Gene position: 1496350-1495559 (Counterclockwise)

Preceding gene: 229917255

Following gene: 229917253

Centisome position: 49.88

GC content: 53.66

Gene sequence:

>792_bases
ATGAGACATGAGATTTGGAACGAGATTCGACAGCAGGCACCGTTGATTCACAACATCACGAACACGGTTGTGGCGAATTT
TTCAGCGAATGGACTGCTCGCAATCGGCGCCTCTCCAGTGATGGCAGACGCGATTGAAGAAGTTGCAGAGATGACATTCG
TGAGCGATGCGCTCGTGTTAAATATCGGGACACTTGGAACAATGACCGAAGCGACGATGATTCGTGCAGGGCAGGCGGCG
AATGAAGCCGGATGTCCCATTGTCCTCGATCCAGTCGGAGTCGGAGCGACGTCATTTCGTCGAGATGTGGTCGAACGCAT
TATGCGTCACGTTCGTGTGTCAGTCATTCGAGGGAATGCAGGAGAGATTGCGACATTGATTGGTGTCGATTGGGCGGCTC
GTGGTGTCGATGCGGGTGACGGTGCATGCGAGCCGAGACAATTGGCGCTTGAAGCAGCGAAACGATACGGATGTGTCGTG
GCCGTGACTGGAAAAGAAGATATGGTCAGTGACGGACATGTCGTCATGGAAGTCCGAGGTGGGACGAAACGGATGACTGA
GACGACCGGTACAGGGTGTTTACTGAGTGCGGTGGTCGGAGCTTGCTTGGCAGTCGAAAAGAATGCCCTCAAAGCGACGG
TGACCGCCATGTCAGGCTACGCCTATTGCGGGGAGCTAGCGAGTGAACGGGCAGAAGGGCCGGGAGACTTCCCGATTCAC
TTTTTAAATGCACTCCATCAATTGACGCAATATACAATCCCGACGAATCGAATCGAGGTGTCGACATCATGA

Upstream 100 bases:

>100_bases
GTTACACAAAACTCTTCTTGAAGAGTTGCTATTACGAAGTCGCTTTTTGGGAAATGGCGTGGACGCTTGAGTCGTGGACA
TTGTCGCAGGAGGTCAACCA

Downstream 100 bases:

>100_bases
GACCGCAAGTATTGACGATTGCAGGATCTGATTCAGGGGGCGGAGCTGGTATTCAAGCCGATCTAAAAACGTTTCAAGAG
CTCGGTGTGTTCGGGACGAG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRHEIWNEIRQQAPLIHNITNTVVANFSANGLLAIGASPVMADAIEEVAEMTFVSDALVLNIGTLGTMTEATMIRAGQAA
NEAGCPIVLDPVGVGATSFRRDVVERIMRHVRVSVIRGNAGEIATLIGVDWAARGVDAGDGACEPRQLALEAAKRYGCVV
AVTGKEDMVSDGHVVMEVRGGTKRMTETTGTGCLLSAVVGACLAVEKNALKATVTAMSGYAYCGELASERAEGPGDFPIH
FLNALHQLTQYTIPTNRIEVSTS

Sequences:

>Translated_263_residues
MRHEIWNEIRQQAPLIHNITNTVVANFSANGLLAIGASPVMADAIEEVAEMTFVSDALVLNIGTLGTMTEATMIRAGQAA
NEAGCPIVLDPVGVGATSFRRDVVERIMRHVRVSVIRGNAGEIATLIGVDWAARGVDAGDGACEPRQLALEAAKRYGCVV
AVTGKEDMVSDGHVVMEVRGGTKRMTETTGTGCLLSAVVGACLAVEKNALKATVTAMSGYAYCGELASERAEGPGDFPIH
FLNALHQLTQYTIPTNRIEVSTS
>Mature_263_residues
MRHEIWNEIRQQAPLIHNITNTVVANFSANGLLAIGASPVMADAIEEVAEMTFVSDALVLNIGTLGTMTEATMIRAGQAA
NEAGCPIVLDPVGVGATSFRRDVVERIMRHVRVSVIRGNAGEIATLIGVDWAARGVDAGDGACEPRQLALEAAKRYGCVV
AVTGKEDMVSDGHVVMEVRGGTKRMTETTGTGCLLSAVVGACLAVEKNALKATVTAMSGYAYCGELASERAEGPGDFPIH
FLNALHQLTQYTIPTNRIEVSTS

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=243, Percent_Identity=43.2098765432099, Blast_Score=181, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6325042, Length=281, Percent_Identity=29.5373665480427, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_EXISA (C4KZE2)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885900.1
- ProteinModelPortal:   C4KZE2
- GeneID:   7869845
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1529
- OMA:   AIRGNAG
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27753; Mature: 27753

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: BINDING 41-41 BINDING 117-117 BINDING 163-163 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHEIWNEIRQQAPLIHNITNTVVANFSANGLLAIGASPVMADAIEEVAEMTFVSDALVL
CCHHHHHHHHHHCCHHHHHHHHEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHEE
NIGTLGTMTEATMIRAGQAANEAGCPIVLDPVGVGATSFRRDVVERIMRHVRVSVIRGNA
EECCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCC
GEIATLIGVDWAARGVDAGDGACEPRQLALEAAKRYGCVVAVTGKEDMVSDGHVVMEVRG
CCEEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEECC
GTKRMTETTGTGCLLSAVVGACLAVEKNALKATVTAMSGYAYCGELASERAEGPGDFPIH
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHH
FLNALHQLTQYTIPTNRIEVSTS
HHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MRHEIWNEIRQQAPLIHNITNTVVANFSANGLLAIGASPVMADAIEEVAEMTFVSDALVL
CCHHHHHHHHHHCCHHHHHHHHEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHEE
NIGTLGTMTEATMIRAGQAANEAGCPIVLDPVGVGATSFRRDVVERIMRHVRVSVIRGNA
EECCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCC
GEIATLIGVDWAARGVDAGDGACEPRQLALEAAKRYGCVVAVTGKEDMVSDGHVVMEVRG
CCEEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEECC
GTKRMTETTGTGCLLSAVVGACLAVEKNALKATVTAMSGYAYCGELASERAEGPGDFPIH
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHH
FLNALHQLTQYTIPTNRIEVSTS
HHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA