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The map label for this gene is ybbF [H]

Identifier: 229917118

GI number: 229917118

Start: 1363153

End: 1364526

Strand: Reverse

Name: ybbF [H]

Synonym: EAT1b_1392

Alternate gene names: 229917118

Gene position: 1364526-1363153 (Counterclockwise)

Preceding gene: 229917119

Following gene: 229917117

Centisome position: 45.49

GC content: 52.98

Gene sequence:

>1374_bases
ATGAAGAAAGAAGAGGTTTTAGCACGGGATATCCTCGCACATGTCGGGGGGAAAGAGAACATCCGTCAACTCGCGCATTG
TATGACACGTGTTCGACTGACGCTGAAGGATGGGACGAAGGCAGAGATTGACGCATTGAAAAAGTTCGACGGGGTCATGG
GTGTCATCAATGATGAGACGCTGCAAATCGTCGTCGGTCCAGGTACTGTGAATCGGGTCGCGGATTATATGAGTCATGAG
ACAGGGCTCGCAATCGGGGAAGAGTCAGTTGATGCGAACCTGACGTTAGAGGAGCGTGCGGCGATTGAACGCCAGAAGGT
GAAAGAAAAACAAAAGTCACCGTTCAAACGATTCCTTCGCCGCCTCGGGAATATCTTCATTCCGCTCATTCCGGGACTTG
TCGCATCGGGGATCATTAACGGGGCAGCGAACTTTGCCGTCAACGCCGGCGTCGACCGGACGGAAACGTGGATGCAGATT
CTACTCGTGCTCGGTAGCACGGTCTTCGCGTACTTAGCCATCCTTGTGGGTTGGAACACGGCAAAAGAGTTTGGAGGAAC
GCCGGTACTCGGGGCGATTGCCGGGGGGATCCTGTTCAACCCGCTCCTTGCGGACATCGTCATTTATGGGGAGCCGCTCG
TCGTCGGGCGTGGTGGATTATTCGGTGTTATTTTTGCCGGTTGGCTCATGACGTATATCGAGAAGCACATTCGTCGCTTC
ATGCCGGTATCTATTGATATTATTTTCACACCACTCTTTACAGTCTTGATTGTTGGATTTACTGCTTTATATGCGATCAT
GCCGGTAGCTGGCGTATTGTCAGACGGTATCATGCGAGGAATCAATGCGACGCTTGAATTCGGGGGACCTGTCGCAGGGG
CACTCTTGGCCGGATTCTTCTTACCGCTTGTCATGGTCGGATTGCATCACGGTTTGACGCCAATCCATTTGGAACTGTTG
AACACGGTCGGAACGACGGCTCTTCTCCCAATCTTGGCGATGGCCGGGGCGGGTCAAGTCGGTGCAGCGCTTGCCATCTT
TGTGAAGACGCGCAACCCACGCCTTCGCAATATTATCAAAGGGGCCATTCCGGTAGGTTTCCTTGGAATCGGGGAGCCGC
TCCTATACGGGGTCACGCTTCCGCTCGGACGTCCATTCTTAACGGCATGTCTCGGGGCCGCGGTCGGTGGTGCCTTCCAA
GCGACAATGAAGACAGCGGCGCTCGGGGTTGGTGTTTCCGGTCTATCGCTCACACCATTGATTGATAATGGCATGTATTT
GACGTACATTGCCGGACTATTGATTTCATATACGGCAGGCTTCCTGTTCACTTATTTCTTCGGTTTCAAAGAAGAGATGG
CGGACGGAATTTGA

Upstream 100 bases:

>100_bases
ATCGTCATGATTTTAGCGGACGTCTCTTATGAGGAGGCTGTCAAACGATTAGAGCGCGCGAACGGATTCGTACGCGAGGC
GCTATAAGGGGGAAATCGAG

Downstream 100 bases:

>100_bases
CAAACAAAGATGGCTCGAGTCGAGTCATCTTTTTCTCTACTTTCGGAAAGGTGGATTCACATGAGAGGAATTTCGGTTTA
CTTAAGTGAGGAACTGACGT

Product: phosphotransferase system EIIC

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKKEEVLARDILAHVGGKENIRQLAHCMTRVRLTLKDGTKAEIDALKKFDGVMGVINDETLQIVVGPGTVNRVADYMSHE
TGLAIGEESVDANLTLEERAAIERQKVKEKQKSPFKRFLRRLGNIFIPLIPGLVASGIINGAANFAVNAGVDRTETWMQI
LLVLGSTVFAYLAILVGWNTAKEFGGTPVLGAIAGGILFNPLLADIVIYGEPLVVGRGGLFGVIFAGWLMTYIEKHIRRF
MPVSIDIIFTPLFTVLIVGFTALYAIMPVAGVLSDGIMRGINATLEFGGPVAGALLAGFFLPLVMVGLHHGLTPIHLELL
NTVGTTALLPILAMAGAGQVGAALAIFVKTRNPRLRNIIKGAIPVGFLGIGEPLLYGVTLPLGRPFLTACLGAAVGGAFQ
ATMKTAALGVGVSGLSLTPLIDNGMYLTYIAGLLISYTAGFLFTYFFGFKEEMADGI

Sequences:

>Translated_457_residues
MKKEEVLARDILAHVGGKENIRQLAHCMTRVRLTLKDGTKAEIDALKKFDGVMGVINDETLQIVVGPGTVNRVADYMSHE
TGLAIGEESVDANLTLEERAAIERQKVKEKQKSPFKRFLRRLGNIFIPLIPGLVASGIINGAANFAVNAGVDRTETWMQI
LLVLGSTVFAYLAILVGWNTAKEFGGTPVLGAIAGGILFNPLLADIVIYGEPLVVGRGGLFGVIFAGWLMTYIEKHIRRF
MPVSIDIIFTPLFTVLIVGFTALYAIMPVAGVLSDGIMRGINATLEFGGPVAGALLAGFFLPLVMVGLHHGLTPIHLELL
NTVGTTALLPILAMAGAGQVGAALAIFVKTRNPRLRNIIKGAIPVGFLGIGEPLLYGVTLPLGRPFLTACLGAAVGGAFQ
ATMKTAALGVGVSGLSLTPLIDNGMYLTYIAGLLISYTAGFLFTYFFGFKEEMADGI
>Mature_457_residues
MKKEEVLARDILAHVGGKENIRQLAHCMTRVRLTLKDGTKAEIDALKKFDGVMGVINDETLQIVVGPGTVNRVADYMSHE
TGLAIGEESVDANLTLEERAAIERQKVKEKQKSPFKRFLRRLGNIFIPLIPGLVASGIINGAANFAVNAGVDRTETWMQI
LLVLGSTVFAYLAILVGWNTAKEFGGTPVLGAIAGGILFNPLLADIVIYGEPLVVGRGGLFGVIFAGWLMTYIEKHIRRF
MPVSIDIIFTPLFTVLIVGFTALYAIMPVAGVLSDGIMRGINATLEFGGPVAGALLAGFFLPLVMVGLHHGLTPIHLELL
NTVGTTALLPILAMAGAGQVGAALAIFVKTRNPRLRNIIKGAIPVGFLGIGEPLLYGVTLPLGRPFLTACLGAAVGGAFQ
ATMKTAALGVGVSGLSLTPLIDNGMYLTYIAGLLISYTAGFLFTYFFGFKEEMADGI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=485, Percent_Identity=37.7319587628866, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1790159, Length=426, Percent_Identity=29.3427230046948, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI2367362, Length=429, Percent_Identity=26.1072261072261, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI48994906, Length=480, Percent_Identity=25.8333333333333, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 48595; Mature: 48595

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEEVLARDILAHVGGKENIRQLAHCMTRVRLTLKDGTKAEIDALKKFDGVMGVINDET
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCEEEECCCE
LQIVVGPGTVNRVADYMSHETGLAIGEESVDANLTLEERAAIERQKVKEKQKSPFKRFLR
EEEEECCCHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
RLGNIFIPLIPGLVASGIINGAANFAVNAGVDRTETWMQILLVLGSTVFAYLAILVGWNT
HHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AKEFGGTPVLGAIAGGILFNPLLADIVIYGEPLVVGRGGLFGVIFAGWLMTYIEKHIRRF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
MPVSIDIIFTPLFTVLIVGFTALYAIMPVAGVLSDGIMRGINATLEFGGPVAGALLAGFF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHH
LPLVMVGLHHGLTPIHLELLNTVGTTALLPILAMAGAGQVGAALAIFVKTRNPRLRNIIK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCHHHHHHH
GAIPVGFLGIGEPLLYGVTLPLGRPFLTACLGAAVGGAFQATMKTAALGVGVSGLSLTPL
HCCCCCCCCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCH
IDNGMYLTYIAGLLISYTAGFLFTYFFGFKEEMADGI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKEEVLARDILAHVGGKENIRQLAHCMTRVRLTLKDGTKAEIDALKKFDGVMGVINDET
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCEEEECCCE
LQIVVGPGTVNRVADYMSHETGLAIGEESVDANLTLEERAAIERQKVKEKQKSPFKRFLR
EEEEECCCHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
RLGNIFIPLIPGLVASGIINGAANFAVNAGVDRTETWMQILLVLGSTVFAYLAILVGWNT
HHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AKEFGGTPVLGAIAGGILFNPLLADIVIYGEPLVVGRGGLFGVIFAGWLMTYIEKHIRRF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
MPVSIDIIFTPLFTVLIVGFTALYAIMPVAGVLSDGIMRGINATLEFGGPVAGALLAGFF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHH
LPLVMVGLHHGLTPIHLELLNTVGTTALLPILAMAGAGQVGAALAIFVKTRNPRLRNIIK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCHHHHHHH
GAIPVGFLGIGEPLLYGVTLPLGRPFLTACLGAAVGGAFQATMKTAALGVGVSGLSLTPL
HCCCCCCCCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCH
IDNGMYLTYIAGLLISYTAGFLFTYFFGFKEEMADGI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]