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The map label for this gene is ywkE [H]

Identifier: 229917063

GI number: 229917063

Start: 1306918

End: 1307778

Strand: Reverse

Name: ywkE [H]

Synonym: EAT1b_1337

Alternate gene names: 229917063

Gene position: 1307778-1306918 (Counterclockwise)

Preceding gene: 229917064

Following gene: 229917062

Centisome position: 43.59

GC content: 51.57

Gene sequence:

>861_bases
ATGATGCGGATTGCCGCGCTACTTAAACAGACGGAAGAACGGCTAGATGAAGCTGGACGTGACCGTGCGGCTGCCGAGTG
GTGGCTCATACATGTGCTACAAGTCGACCGCACGGGTCTTCTCGTTCGTTTGTCGGATGAATTGTCCTCAGATGAAGCGG
CACGGTTCGAGGCGGGAATCGAGCGTTTATTGATGGGAGAACCGGTACAGCACGTGATTGGACATGCTCCCTTTTACGGG
CGCTCGTTTGAAGTGAACCGGGACGTGTTGATTCCGAGACCAGAAACGGAAGAATTGATTGATTGGGTGCTTGGGCAACT
TCGTCACGTGAAGGATGATGAGATTGTCGATGTCGGAACGGGAAGCGGAGCAATCGCAATCACGTTGAGCCTTGAGCTCG
GAGTACGCGTGCAAACCGTTGATATTTCGCGTGAGGCGATTGAAGTCGCTAAGCGAAATGCAGCCGCGCTCGGGGCAACT
GTACAGTTTTATGAAGGCGACGGGCTCGCCCCGATTGCGGACCACTCGATTCGCGTTCTCGTCTCAAATCCACCCTATAT
CGAGGCGGATGAATTGTTGGATGAGACGGTCGTCGGCTACGAACCGCATCTCGCCCTCTTCGGAGGAAAAGATGGGCTCG
ACATGTATCGGCAATTGATTGCAGAGTCGGTGCGCGTTTTACGTGCCGATTGGCATTTGATTGCGTTTGAAATCGGGTAT
AACCAAGGACAAGATGTGAAATCATTACTGTCTGAACGTTATCCTGAAGCAGAAACGGGTATTTTAAAAGATATAAACGG
CAAGGACCGTATCGTCTATGCCGTAAGGGAAGGGATTGCGTATGGAAACGAAATGGATTAA

Upstream 100 bases:

>100_bases
TGACCCTTCAAAAGTTGGACCGTGTCCTAGCAGGTGAACTCGAAGACATTATCGACGCACTCATCATGGACGAACAGGCA
CGTCTGATGGAGGATGCTGA

Downstream 100 bases:

>100_bases
TTCGAATGAGATTGAAGAGGGGGTACGTCTTCTTCAAGCGGGTGAAATCATCGCCATGCCGACAGAAACCGTCTATGGTC
TCGCGGGAGATGCCATGAAC

Product: modification methylase, HemK family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MMRIAALLKQTEERLDEAGRDRAAAEWWLIHVLQVDRTGLLVRLSDELSSDEAARFEAGIERLLMGEPVQHVIGHAPFYG
RSFEVNRDVLIPRPETEELIDWVLGQLRHVKDDEIVDVGTGSGAIAITLSLELGVRVQTVDISREAIEVAKRNAAALGAT
VQFYEGDGLAPIADHSIRVLVSNPPYIEADELLDETVVGYEPHLALFGGKDGLDMYRQLIAESVRVLRADWHLIAFEIGY
NQGQDVKSLLSERYPEAETGILKDINGKDRIVYAVREGIAYGNEMD

Sequences:

>Translated_286_residues
MMRIAALLKQTEERLDEAGRDRAAAEWWLIHVLQVDRTGLLVRLSDELSSDEAARFEAGIERLLMGEPVQHVIGHAPFYG
RSFEVNRDVLIPRPETEELIDWVLGQLRHVKDDEIVDVGTGSGAIAITLSLELGVRVQTVDISREAIEVAKRNAAALGAT
VQFYEGDGLAPIADHSIRVLVSNPPYIEADELLDETVVGYEPHLALFGGKDGLDMYRQLIAESVRVLRADWHLIAFEIGY
NQGQDVKSLLSERYPEAETGILKDINGKDRIVYAVREGIAYGNEMD
>Mature_286_residues
MMRIAALLKQTEERLDEAGRDRAAAEWWLIHVLQVDRTGLLVRLSDELSSDEAARFEAGIERLLMGEPVQHVIGHAPFYG
RSFEVNRDVLIPRPETEELIDWVLGQLRHVKDDEIVDVGTGSGAIAITLSLELGVRVQTVDISREAIEVAKRNAAALGAT
VQFYEGDGLAPIADHSIRVLVSNPPYIEADELLDETVVGYEPHLALFGGKDGLDMYRQLIAESVRVLRADWHLIAFEIGY
NQGQDVKSLLSERYPEAETGILKDINGKDRIVYAVREGIAYGNEMD

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=282, Percent_Identity=28.7234042553192, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787463, Length=269, Percent_Identity=30.4832713754647, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082085, Length=242, Percent_Identity=30.9917355371901, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582226, Length=272, Percent_Identity=31.9852941176471, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31842; Mature: 31842

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIAALLKQTEERLDEAGRDRAAAEWWLIHVLQVDRTGLLVRLSDELSSDEAARFEAGI
CHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
ERLLMGEPVQHVIGHAPFYGRSFEVNRDVLIPRPETEELIDWVLGQLRHVKDDEIVDVGT
HHHHCCCCHHHHHCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHCCCCCCEEEECC
GSGAIAITLSLELGVRVQTVDISREAIEVAKRNAAALGATVQFYEGDGLAPIADHSIRVL
CCCEEEEEEEEEECEEEEEEECCHHHHHHHHHCHHHHCCEEEEECCCCCCCCCCCCEEEE
VSNPPYIEADELLDETVVGYEPHLALFGGKDGLDMYRQLIAESVRVLRADWHLIAFEIGY
ECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECC
NQGQDVKSLLSERYPEAETGILKDINGKDRIVYAVREGIAYGNEMD
CCCHHHHHHHHHCCCCCCCCHHCCCCCCCCEEEEEHHHHCCCCCCC
>Mature Secondary Structure
MMRIAALLKQTEERLDEAGRDRAAAEWWLIHVLQVDRTGLLVRLSDELSSDEAARFEAGI
CHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
ERLLMGEPVQHVIGHAPFYGRSFEVNRDVLIPRPETEELIDWVLGQLRHVKDDEIVDVGT
HHHHCCCCHHHHHCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHCCCCCCEEEECC
GSGAIAITLSLELGVRVQTVDISREAIEVAKRNAAALGATVQFYEGDGLAPIADHSIRVL
CCCEEEEEEEEEECEEEEEEECCHHHHHHHHHCHHHHCCEEEEECCCCCCCCCCCCEEEE
VSNPPYIEADELLDETVVGYEPHLALFGGKDGLDMYRQLIAESVRVLRADWHLIAFEIGY
ECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECC
NQGQDVKSLLSERYPEAETGILKDINGKDRIVYAVREGIAYGNEMD
CCCHHHHHHHHHCCCCCCCCHHCCCCCCCCEEEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]