Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 229917064

GI number: 229917064

Start: 1307775

End: 1308845

Strand: Reverse

Name: prfA

Synonym: EAT1b_1338

Alternate gene names: 229917064

Gene position: 1308845-1307775 (Counterclockwise)

Preceding gene: 229917065

Following gene: 229917063

Centisome position: 43.63

GC content: 50.33

Gene sequence:

>1071_bases
ATGTTTGACCGTTTAAGTGTATTAGAAGATCGTTACATGCAATTGAATGAAATGCTCGCCGATCCTGAGGTGCTCAGTGA
CTCGACGAAGTTGAGACAATATTCGAAAGAGCAGGCACAACTAGAAGAGACGGTACAGATGTATCGTCAGTATAAAGAGA
CAAGTCAGGCGTTCAAGGAAGCGAAATCGATGCTTGAAGACCGGACGCTCGACGCCGAGATGCGCGAATTGGCAAAAGAA
GAGATGAACCTCCTCGAGCCAGAAGTCAAAGAGCTAGAGGCGAAACTGCGGATTTTATTGCTTCCAAAAGACCCGAACGA
TGAAAAGAACGTCATCGTCGAAGTCCGAGGGGCGGCAGGCGGAGACGAAGCGGCGCTCTTTGCGGGAGATTTGTATAAGA
TGTATACGCGATTTGCTGAACGTCAAAATTGGAAAGTGGAACTGATTGACGCGAACTATACCGAACTCGGTGGATTTAAA
GAAGTGACGTTCATGATCAACGGGGCAGGCGCCTATTCAAAGCTCAAGTTTGAGAACGGAGCACATCGTGTGCAGCGGGT
CCCGTCGACGGAGTCGGGCGGACGAATCCATACGTCCACAGCTACGGTAGCCGTTCTCCCAGAGGCGGAGGATGTCGAAG
TGCACATTGATATGAAGGACGTACGGGTCGATACGTTCACTTCATCTGGTCCAGGTGGGCAATCGGTCAACACGACGCAA
TCGGCTGTCCGTTTGACACACATCCCGTCAGGACTCGTCGTATCGTGCCAAGATGAGAAGTCGCAACATAAAAATAAAGA
CAAGGCGATGAAGGTCCTACGGGCACGTCTCTATGACAAGATGCAAAGCGAACATATGGAAGAATTATCGGCACAGCGTA
AATCGGCTGTCGGAACAGGTGACCGTTCCGAACGGATCCGCACGTATAACTTCCCGCAAAGTCGTGTCACAGATCATCGG
ATCGGCTTGACCCTTCAAAAGTTGGACCGTGTCCTAGCAGGTGAACTCGAAGACATTATCGACGCACTCATCATGGACGA
ACAGGCACGTCTGATGGAGGATGCTGAATGA

Upstream 100 bases:

>100_bases
CATACGGTGGTAGACTGATGAATCGACGGACCGCCCTCGGCGGTCCGTTTTTTTGCTATAATAGATAGAGTTTGATTTGA
CGGAGAAAGGACGGATCATC

Downstream 100 bases:

>100_bases
TGCGGATTGCCGCGCTACTTAAACAGACGGAAGAACGGCTAGATGAAGCTGGACGTGACCGTGCGGCTGCCGAGTGGTGG
CTCATACATGTGCTACAAGT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MFDRLSVLEDRYMQLNEMLADPEVLSDSTKLRQYSKEQAQLEETVQMYRQYKETSQAFKEAKSMLEDRTLDAEMRELAKE
EMNLLEPEVKELEAKLRILLLPKDPNDEKNVIVEVRGAAGGDEAALFAGDLYKMYTRFAERQNWKVELIDANYTELGGFK
EVTFMINGAGAYSKLKFENGAHRVQRVPSTESGGRIHTSTATVAVLPEAEDVEVHIDMKDVRVDTFTSSGPGGQSVNTTQ
SAVRLTHIPSGLVVSCQDEKSQHKNKDKAMKVLRARLYDKMQSEHMEELSAQRKSAVGTGDRSERIRTYNFPQSRVTDHR
IGLTLQKLDRVLAGELEDIIDALIMDEQARLMEDAE

Sequences:

>Translated_356_residues
MFDRLSVLEDRYMQLNEMLADPEVLSDSTKLRQYSKEQAQLEETVQMYRQYKETSQAFKEAKSMLEDRTLDAEMRELAKE
EMNLLEPEVKELEAKLRILLLPKDPNDEKNVIVEVRGAAGGDEAALFAGDLYKMYTRFAERQNWKVELIDANYTELGGFK
EVTFMINGAGAYSKLKFENGAHRVQRVPSTESGGRIHTSTATVAVLPEAEDVEVHIDMKDVRVDTFTSSGPGGQSVNTTQ
SAVRLTHIPSGLVVSCQDEKSQHKNKDKAMKVLRARLYDKMQSEHMEELSAQRKSAVGTGDRSERIRTYNFPQSRVTDHR
IGLTLQKLDRVLAGELEDIIDALIMDEQARLMEDAE
>Mature_356_residues
MFDRLSVLEDRYMQLNEMLADPEVLSDSTKLRQYSKEQAQLEETVQMYRQYKETSQAFKEAKSMLEDRTLDAEMRELAKE
EMNLLEPEVKELEAKLRILLLPKDPNDEKNVIVEVRGAAGGDEAALFAGDLYKMYTRFAERQNWKVELIDANYTELGGFK
EVTFMINGAGAYSKLKFENGAHRVQRVPSTESGGRIHTSTATVAVLPEAEDVEVHIDMKDVRVDTFTSSGPGGQSVNTTQ
SAVRLTHIPSGLVVSCQDEKSQHKNKDKAMKVLRARLYDKMQSEHMEELSAQRKSAVGTGDRSERIRTYNFPQSRVTDHR
IGLTLQKLDRVLAGELEDIIDALIMDEQARLMEDAE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=331, Percent_Identity=41.0876132930514, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI34577120, Length=329, Percent_Identity=40.4255319148936, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI166795305, Length=236, Percent_Identity=38.5593220338983, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1787462, Length=347, Percent_Identity=49.2795389048991, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=36.5781710914454, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=292, Percent_Identity=38.6986301369863, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=45.5974842767296, Blast_Score=248, Evalue=8e-67,
Organism=Drosophila melanogaster, GI19921226, Length=316, Percent_Identity=43.0379746835443, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_EXISA (C4KYV2)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885710.1
- GeneID:   7870306
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1338
- OMA:   GEAGGYK
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40401; Mature: 40401

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDRLSVLEDRYMQLNEMLADPEVLSDSTKLRQYSKEQAQLEETVQMYRQYKETSQAFKE
CCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKSMLEDRTLDAEMRELAKEEMNLLEPEVKELEAKLRILLLPKDPNDEKNVIVEVRGAAG
HHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCEEEEEECCCCCCCCCEEEEEECCCC
GDEAALFAGDLYKMYTRFAERQNWKVELIDANYTELGGFKEVTFMINGAGAYSKLKFENG
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCEEEEEEEECCCCCEEEEECCC
AHRVQRVPSTESGGRIHTSTATVAVLPEAEDVEVHIDMKDVRVDTFTSSGPGGQSVNTTQ
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEEEHHHEEEEEECCCCCCCCCCCCHH
SAVRLTHIPSGLVVSCQDEKSQHKNKDKAMKVLRARLYDKMQSEHMEELSAQRKSAVGTG
HHEEEEECCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQSRVTDHRIGLTLQKLDRVLAGELEDIIDALIMDEQARLMEDAE
CCCCCCEECCCCHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
>Mature Secondary Structure
MFDRLSVLEDRYMQLNEMLADPEVLSDSTKLRQYSKEQAQLEETVQMYRQYKETSQAFKE
CCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKSMLEDRTLDAEMRELAKEEMNLLEPEVKELEAKLRILLLPKDPNDEKNVIVEVRGAAG
HHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCEEEEEECCCCCCCCCEEEEEECCCC
GDEAALFAGDLYKMYTRFAERQNWKVELIDANYTELGGFKEVTFMINGAGAYSKLKFENG
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCEEEEEEEECCCCCEEEEECCC
AHRVQRVPSTESGGRIHTSTATVAVLPEAEDVEVHIDMKDVRVDTFTSSGPGGQSVNTTQ
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEEEHHHEEEEEECCCCCCCCCCCCHH
SAVRLTHIPSGLVVSCQDEKSQHKNKDKAMKVLRARLYDKMQSEHMEELSAQRKSAVGTG
HHEEEEECCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQSRVTDHRIGLTLQKLDRVLAGELEDIIDALIMDEQARLMEDAE
CCCCCCEECCCCHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA