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The map label for this gene is gntU [H]

Identifier: 229916948

GI number: 229916948

Start: 1200140

End: 1201483

Strand: Direct

Name: gntU [H]

Synonym: EAT1b_1222

Alternate gene names: 229916948

Gene position: 1200140-1201483 (Clockwise)

Preceding gene: 229916947

Following gene: 229916949

Centisome position: 40.01

GC content: 52.68

Gene sequence:

>1344_bases
ATGTCTGGCTCAACACTTATTTTGATCGCCTTAGCCGGGATCTTCTTGCTCTTATTTTTAGTCATTCGCACAAAGCTTCA
TGCATTCGTTTCGTTGTTACTTGTCAGTTTAATCGTCGGGGTCGCAGCCGGCATGCCACTCGGTGATGTCATTGCCTCCA
TCCAAAACGGGATGGGCGGAACGCTCGGCTTCGTCGCCGTCGTCGTCGGTCTTGGCGCGATGTTCGGGAAGATGCTTGAA
GTATCGGGTGGCGCGGAACGTCTTGCCCAGACACTCGTCAGTAAGTTCGGGGAAGACAAGGCACAATGGGCGCTCGGGAT
TACCGGATTTATCGTCGCCGTCCCGGTCTTCTTTGATGTCGGTTTTATCATTCTCGTTCCAATCGTATACGGTCTCGCCA
AAAAGACGGGACGTTCGCTTCTTTACTATGGGATTCCGCTCCTTGCGGGTCTCGCAGTCACACACAGCTTTATCCCACCG
ACACCTGGTCCGATCGCTGTCGCGAACTTGATCGGTGCCGACCTCGGTTGGGTCATCTTGTTCGGTGTCATCGCCGGGAT
TCCAGCCATGATTTTAGCCGGTCCTGTTTTTGGGAAGTATATTGCTAAGAAGATTCATGTGACGATTCCGGATTACATGG
AATTCAAAGAAATCGACACATCGAAAGAACTACCAAGCTTCAAAATGATCTTGTCGCTCATCTTGATTCCGCTCGTGTTG
ATTTTAGCGAACACGCTCTCTGCGGTTCTTCTAGACGAAGGCAACGCGATTCGTGACTTCTTGACGTTCCTCGGACACCC
GTTCGTCGCCTTGACGATCGCGACGCTTCTCACGTTCGTCTTCCTCGGGACACGCCGTGGGTATTCACGTGACGAAGTAC
AAGAAATTGCAACGAAAGCACTCGAGCCAGCCGGGATCATCATCCTCGTCACAGGGGCCGGTGGTGTGTTTAAACAAGTG
CTCATCGACTCAGGTGTCGGTGACGTACTCGGTGAAATGATGGCCGGTTCACCACTTCCGGCGATTGTCCTCGCCTTCTT
GATTGCAACGGTCGTCCGTGTCGCTCAAGGGTCGGCAACTGTGTCGATGGTCACGGCAGCCGGACTCATCACTCCGCTAC
TCGAAATTCAAAACATCACGGGTGCGGCACTCGGTTTGATCGTCATCGCCATCGCCTCAGGGGCGACCGTCCTCTCACAC
GTCAACGACTCAGGCTTCTGGCTCGTCAACCGTTACTTCGGTCTCGATGTGAAAGACACGCTCAAGTCATGGACGATTAT
GGAAACGATTATCGGACTCACCGGCTTCGCCGTTGTGTTCTTGATTAGCCTATTCATCATCTAA

Upstream 100 bases:

>100_bases
TCAAGAAGTAACATGTGTGGAAAAGGTCACGGGGACATGGCCTTTCCCTTTGTTAAAAACTGTAAACGGTTACATATTCG
GAATTTAGGGGGAATACTCA

Downstream 100 bases:

>100_bases
GTTAGGAAGTGATTGTGTGTATACGATTGGGGTCGATATCGGGACGACGAGTACGAAAGCTGTCCTGTTCAACGGTCATC
AACAACTGGCGGTACATAAC

Product: gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSGSTLILIALAGIFLLLFLVIRTKLHAFVSLLLVSLIVGVAAGMPLGDVIASIQNGMGGTLGFVAVVVGLGAMFGKMLE
VSGGAERLAQTLVSKFGEDKAQWALGITGFIVAVPVFFDVGFIILVPIVYGLAKKTGRSLLYYGIPLLAGLAVTHSFIPP
TPGPIAVANLIGADLGWVILFGVIAGIPAMILAGPVFGKYIAKKIHVTIPDYMEFKEIDTSKELPSFKMILSLILIPLVL
ILANTLSAVLLDEGNAIRDFLTFLGHPFVALTIATLLTFVFLGTRRGYSRDEVQEIATKALEPAGIIILVTGAGGVFKQV
LIDSGVGDVLGEMMAGSPLPAIVLAFLIATVVRVAQGSATVSMVTAAGLITPLLEIQNITGAALGLIVIAIASGATVLSH
VNDSGFWLVNRYFGLDVKDTLKSWTIMETIIGLTGFAVVFLISLFII

Sequences:

>Translated_447_residues
MSGSTLILIALAGIFLLLFLVIRTKLHAFVSLLLVSLIVGVAAGMPLGDVIASIQNGMGGTLGFVAVVVGLGAMFGKMLE
VSGGAERLAQTLVSKFGEDKAQWALGITGFIVAVPVFFDVGFIILVPIVYGLAKKTGRSLLYYGIPLLAGLAVTHSFIPP
TPGPIAVANLIGADLGWVILFGVIAGIPAMILAGPVFGKYIAKKIHVTIPDYMEFKEIDTSKELPSFKMILSLILIPLVL
ILANTLSAVLLDEGNAIRDFLTFLGHPFVALTIATLLTFVFLGTRRGYSRDEVQEIATKALEPAGIIILVTGAGGVFKQV
LIDSGVGDVLGEMMAGSPLPAIVLAFLIATVVRVAQGSATVSMVTAAGLITPLLEIQNITGAALGLIVIAIASGATVLSH
VNDSGFWLVNRYFGLDVKDTLKSWTIMETIIGLTGFAVVFLISLFII
>Mature_446_residues
SGSTLILIALAGIFLLLFLVIRTKLHAFVSLLLVSLIVGVAAGMPLGDVIASIQNGMGGTLGFVAVVVGLGAMFGKMLEV
SGGAERLAQTLVSKFGEDKAQWALGITGFIVAVPVFFDVGFIILVPIVYGLAKKTGRSLLYYGIPLLAGLAVTHSFIPPT
PGPIAVANLIGADLGWVILFGVIAGIPAMILAGPVFGKYIAKKIHVTIPDYMEFKEIDTSKELPSFKMILSLILIPLVLI
LANTLSAVLLDEGNAIRDFLTFLGHPFVALTIATLLTFVFLGTRRGYSRDEVQEIATKALEPAGIIILVTGAGGVFKQVL
IDSGVGDVLGEMMAGSPLPAIVLAFLIATVVRVAQGSATVSMVTAAGLITPLLEIQNITGAALGLIVIAIASGATVLSHV
NDSGFWLVNRYFGLDVKDTLKSWTIMETIIGLTGFAVVFLISLFII

Specific function: Part of the gluconate utilization system Gnt-I; low- affinity intake of gluconate [H]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994939, Length=443, Percent_Identity=45.5981941309255, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI48994935, Length=441, Percent_Identity=42.1768707482993, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1790716, Length=430, Percent_Identity=41.1627906976744, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1789097, Length=457, Percent_Identity=42.4507658643326, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI2367370, Length=453, Percent_Identity=38.6313465783664, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1790776, Length=442, Percent_Identity=35.5203619909502, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788707, Length=444, Percent_Identity=34.9099099099099, Blast_Score=252, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 46845; Mature: 46714

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SGSTLILIALAGIFLLLFLVIRTKLHAFVSLLLVSLIVGVAAGMPLGDVIASIQNGMGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH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HHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]